dadX:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DadX

Synonyms

alanine racemase 2, PLP-binding[1], B1190[2][1], DadB[2][1], AlnB[2][1], AlnA[2][1], DadX[2][1], alanine racemase[2][1] , alnB, dadB, ECK1178, JW1179, msuA?, b1190

Product description

ALARACECAT[2][3];

Component of alanine racemase 2, PLP-binding[3]

Alanine racemase; homodimeric[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009006

F

Seeded from EcoCyc (v14.0)

complete

GO:0006522

alanine metabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01201

P

Seeded from EcoCyc (v14.0)

complete

GO:0006522

alanine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000821

P

Seeded from EcoCyc (v14.0)

complete

GO:0006522

alanine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011079

P

Seeded from EcoCyc (v14.0)

complete

GO:0006522

alanine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020622

P

Seeded from EcoCyc (v14.0)

complete

GO:0008784

alanine racemase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01201

F

Seeded from EcoCyc (v14.0)

complete

GO:0008784

alanine racemase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000821

F

Seeded from EcoCyc (v14.0)

complete

GO:0008784

alanine racemase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011079

F

Seeded from EcoCyc (v14.0)

complete

GO:0008784

alanine racemase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020622

F

Seeded from EcoCyc (v14.0)

complete

GO:0008784

alanine racemase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:5.1.1.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01201

P

Seeded from EcoCyc (v14.0)

complete

GO:0016853

isomerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0413

F

Seeded from EcoCyc (v14.0)

complete

GO:0030170

pyridoxal phosphate binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01201

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of alanine racemase 2, PLP-binding

could be indirect

Protein

groL

PMID:16606699[5]

Experiment(s):EBI-1139129

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1139129

Protein

gatA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ycfD

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

groS

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):19.151211

Protein

dapB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

gabD

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

gcvP

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

deoA

PMID:19402753[6]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTRPIQASLD LQALKQNLSI VRQAATHARV WSVVKANAYG HGIERIWSAI GATDGFALLN
LEEAITLRER GWKGPILMLE GFFHAQDLEI YDQHRLTTCV HSNWQLKALQ NARLKAPLDI
YLKVNSGMNR LGFQPDRVLT VWQQLRAMAN VGEMTLMSHF AEAEHPDGIS GAMARIEQAA
EGLECRRSLS NSAATLWHPE AHFDWVRPGI ILYGASPSGQ WRDIANTGLR PVMTLSSEII
GVQTLKAGER VGYGGRYTAR DEQRIGIVAA GYADGYPRHA PTGTPVLVDG VRTMTVGTVS
MDMLAVDLTP CPQAGIGTPV ELWGKEIKID DVAAAAGTVG YELMCALALR VPVVTV
Length

356

Mol. Wt

38.844 kDa

pI

7.1 (calculated)

Extinction coefficient

62,910 - 63,410 (calc based on 9 Y, 9 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

232..356

PF00842 Alanine racemase, C-terminal domain

PMID:19920124[7]

Domain

9..217

PF01168 Alanine racemase, N-terminal domain

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dadX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129153

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945754

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003997

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P29012

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11408

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11408

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945754

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001374

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1380

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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