cysM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cysM

Mnemonic

Cysteine

Synonyms

ECK2416, b2421, JW2414[1], JW2414

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

54.67 minutes 

MG1655: 2537605..2536694
<gbrowseImage> name=NC_000913:2536694..2537605 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2468325..2467414
<gbrowseImage> name=NC_012967:2467414..2468325 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2422499..2423410
<gbrowseImage> name=NC_012759:2422499..2423410 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2545029..2544118
<gbrowseImage> name=NC_007779:2544118..2545029 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2629370..2628459
<gbrowseImage> name=NC_010473:2628459..2629370 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2536694

Edman degradation

PMID:8663055[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcysM (Keio:JW2414)

deletion

deletion

Auxotrophies

Requires cysteine for growth

PMID:16738554[3]

Shigen

CGSC9922[4]

cysM::Tn5KAN-I-SceI (FB20791)

Insertion at nt 280 in Minus orientation

Auxotrophies

Requires cysteine for growth

PMID:15262929[5]

E. coli Genome Project:FB20791

contains pKD46

cysM::Tn5KAN-I-SceI (FB20792)

Insertion at nt 280 in Minus orientation

Auxotrophies

Requires cysteine for growth

PMID:15262929[5]

E. coli Genome Project:FB20792

does not contain pKD46

ΔcysM750::kan

deletion

deletion

Auxotrophies

Requires cysteine for growth

PMID:16738554[3]

CGSC:100500


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2414

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGTACATTAGAACAAACAAT

Primer 2:CCAATCCCCGCCCCCTGGCTAAA

12G2

Kohara Phage

Genobase

PMID:3038334[7]

5F7

Kohara Phage

Genobase

PMID:3038334[7]

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[4]

est. P1 cotransduction: 36% [8]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: 6% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10193

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10193

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000188

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946888

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0190

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007982

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Flint, DH et al. (1996) Studies on the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase in escherichia coli crude extract. Isolation of O-acetylserine sulfhydrylases A and B and beta-cystathionase based on their ability to mobilize sulfur from cysteine and to participate in Fe-S cluster synthesis. J. Biol. Chem. 271 16053-67 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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