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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	cysH
Gene Synonym(s)	ECK2757, b2762, JW2732^[1], JW2732
Product Desc.	CysH^[2]^[3]; Component of 3'-phospho-adenylylsulfate reductase^[2]^[3] Phosphoadenylyl sulfate (PAPS) reductase^[4]
Product Synonyms(s)	3'-phosphoadenosine 5'-phosphosulfate reductase^[1], B2762^[2]^[1], CysH^[2]^[1] , ECK2757, JW2732, b2762
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cysJIH^[2], OP00106, cysJHI
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cysH
Mnemonic	Cysteine
Synonyms	ECK2757, b2762, JW2732^[1], JW2732
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	62.19 minutes	MG1655: 2886334..2885600 <gbrowseImage> name=NC_000913:2885600..2886334 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2776813..2776079 <gbrowseImage> name=NC_012967:2776079..2776813 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2771412..2772146 <gbrowseImage> name=NC_012759:2771412..2772146 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2886968..2886234 <gbrowseImage> name=NC_007779:2886234..2886968 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2978876..2978142 <gbrowseImage> name=NC_010473:2978142..2978876 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2885603	Edman degradation	PMID:2404794
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
cysHC239S		C239S	Auxotrophies	5-fold reduction in Vmax			seeded from UniProt:P17854
cysHY209F		Y209F	Auxotrophies	Reduction in Vmax			seeded from UniProt:P17854
ΔcysH (Keio:JW2732)	deletion	deletion	Auxotrophies	Requires cysteine for growth	PMID:16738554	Shigen CGSC10148^[5]
cysH::Tn5KAN-I-SceI (FB20924)	Insertion at nt 265 in Minus orientation		Auxotrophies	Requires cysteine for growth	PMID:15262929	E. coli Genome Project:FB20924	contains pKD46
cysH56			Auxotrophies	Requires cysteine for growth		CGSC:5746
cysH57			Auxotrophies	Requires cysteine for growth		CGSC:11991
ΔcysH764::kan	deletion	deletion	Auxotrophies	Requires cysteine for growth	PMID:16738554	CGSC:100492
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2732	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCCAAACTCGATCTAAACGC Primer 2:CCgCCTTCGTGTAACCCACATTC
6C8	Kohara Phage	Genobase	PMID:3038334
9A10	Kohara Phage	Genobase	PMID:3038334
1B3	Kohara Phage	Genobase	PMID:3038334
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 99% ^[6] Synonyms:cysC95::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10189	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10189	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000184	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947230	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0186	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009057	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CysH
Synonyms	3'-phosphoadenosine 5'-phosphosulfate reductase^[1], B2762^[2]^[1], CysH^[2]^[1] , ECK2757, JW2732, b2762
Product description	CysH^[2]^[3]; Component of 3'-phospho-adenylylsulfate reductase^[2]^[3] Phosphoadenylyl sulfate (PAPS) reductase^[4]
EC number (for enzymes)	1.8.4.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004604	phosphoadenylyl-sulfate reductase (thioredoxin) activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00063	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004604	phosphoadenylyl-sulfate reductase (thioredoxin) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004511	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004604	phosphoadenylyl-sulfate reductase (thioredoxin) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011800	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004604	phosphoadenylyl-sulfate reductase (thioredoxin) activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.8.4.8	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00063	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002500	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002500	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019344	cysteine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011800	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019379	sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00063	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019379	sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004511	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004511	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011800	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	groL		PMID:16606699	Experiment(s):EBI-1143874
Protein	Subunits of 3'-phospho-adenylylsulfate reductase	could be indirect
Protein	Subunits of 3'-phospho-adenylylsulfate reductase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG	at EcoCyc MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG
Length	244	244
Mol. Wt	27.976 kDa	27.976 kDa
pI	5.6 (calculated)	5.6 (calculated)
Extinction coefficient	53,400 - 53,525 (calc based on 10 Y, 7 W, and 1 C residues)	53,400 - 53,525 (calc based on 10 Y, 7 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P17854
Domain	47..220	PF01507 Phosphoadenosine phosphosulfate reductase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cysH taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130669	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947230	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009057	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P17854	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10189	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10189	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947230	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000184	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0186	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	29	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1579	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	5175	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cysJIH
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2886314..2886354 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2762 (EcoliWiki Page)	NCBI GEO profiles for cysH
microarray	GenExpDB:b2762 (EcoliWiki Page)	Summary of data for cysH from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2886254..2886412) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:C12^[7]
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Notes

Accessions Related to cysH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10189	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0186	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2762
EcoGene	EcoGene:EG10189	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000184	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009057	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000378 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT4G04610 (score: 1.000; bootstrap: 100%) AT4G21990 (score: 0.826) AT1G62180 (score: 0.730)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0032522 (score: 1.000; bootstrap: 100%) FBgn0030431 (score: 0.283)	From Inparanoid:20070104
Oryza gramene	Q5EUC9 (score: 1.000; bootstrap: 100%) Q5EUC8 (score: 0.961) Q6Z4A7 (score: 0.853) Q93Y96 (score: 0.624)	From Inparanoid:20070104
Saccharomyces cerevisiae	YPR167C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC13G76 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	CYSH	From SHIGELLACYC
E. coli O157	CYSH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:52402
Pfam (EcoliWiki Page)	PF01507 Phosphoadenosine phosphosulfate reductase family
EcoCyc	EcoCyc:EG10189	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10189	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000184	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0186	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009057	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cysH:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cysH Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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