cysC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

cysC

Gene Synonym(s)

ECK2745, b2750, JW2720[1], JW2720

Product Desc.

CysC[2][3];

Component of adenylylsulfate kinase[2][3]

Adenylylsulfate kinase, APS kinase[4]

Product Synonyms(s)

adenosine 5'-phosphosulfate kinase[1], B2750[2][1], CysC[2][1] , ECK2745, JW2720, b2750

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): cysDNC[2], cysD, cysNC

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cysC

Mnemonic

Cysteine

Synonyms

ECK2745, b2750, JW2720[1], JW2720

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

61.89 minutes, 61.89 minutes 

MG1655: 2872014..2871409
<gbrowseImage> name=NC_000913:2871409..2872014 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2768853..2768248
<gbrowseImage> name=NC_012967:2768248..2768853 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2757221..2757826
<gbrowseImage> name=NC_012759:2757221..2757826 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2872648..2872043
<gbrowseImage> name=NC_007779:2872043..2872648 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2964556..2963951
<gbrowseImage> name=NC_010473:2963951..2964556 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2871412

Edman degradation

PMID:1316900


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcysC (Keio:JW2720)

deletion

deletion

Auxotrophies

Requires cysteine for growth

PMID:16738554

Shigen

CGSC10139[5]

cysC::Tn5KAN-I-SceI (FB20921)

Insertion at nt 179 in Minus orientation

Auxotrophies

Requires cysteine for growth

PMID:15262929

E. coli Genome Project:FB20921

does not contain pKD46

cysC::Tn5KAN-I-SceI (FB20922)

Insertion at nt 179 in Minus orientation

Auxotrophies

Requires cysteine for growth

PMID:15262929

E. coli Genome Project:FB20922

contains pKD46

cysC92

Auxotrophies

Requires cysteine for growth

CGSC:8514

cysC9

Auxotrophies

Requires cysteine for growth

CGSC:13153

cysC3

Auxotrophies

Requires cysteine for growth

CGSC:13161

cysC45

Auxotrophies

Requires cysteine for growth

CGSC:13999

ΔcysC752::kan

deletion

deletion

Auxotrophies

Requires cysteine for growth

PMID:16738554

CGSC:100484


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2720

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCGCTGCATGACGAAAACGT

Primer 2:CCGGATCTGATAATATCGTTCTG

1B5

Kohara Phage

Genobase

PMID:3038334

6C8

Kohara Phage

Genobase

PMID:3038334

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: 13% [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[5]

est. P1 cotransduction: 60% [6]
Synonyms:cysC95::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10185

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10185

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000180

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947221

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0182

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009023

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CysC

Synonyms

adenosine 5'-phosphosulfate kinase[1], B2750[2][1], CysC[2][1] , ECK2745, JW2720, b2750

Product description

CysC[2][3];

Component of adenylylsulfate kinase[2][3]

Adenylylsulfate kinase, APS kinase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000103

sulfate assimilation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00065

P

Seeded from EcoCyc (v14.0)

complete

GO:0000103

sulfate assimilation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002891

P

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004020

adenylylsulfate kinase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00065

F

Seeded from EcoCyc (v14.0)

complete

GO:0004020

adenylylsulfate kinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.25

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00065

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002891

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002891

F

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of adenylylsulfate kinase

could be indirect

Protein

rpsE

PMID:16606699

Experiment(s):EBI-1143817

Protein

fur

PMID:16606699

Experiment(s):EBI-1143817

Protein

ispD

PMID:16606699

Experiment(s):EBI-1143817

Protein

ftsQ

PMID:16606699

Experiment(s):EBI-1143817

Protein

ydhT

PMID:16606699

Experiment(s):EBI-1143817

Protein

bfd

PMID:16606699

Experiment(s):EBI-1143817

Protein

ygiT

PMID:16606699

Experiment(s):EBI-1143817

Protein

recF

PMID:16606699

Experiment(s):EBI-1143817

Protein

chaC

PMID:16606699

Experiment(s):EBI-1143817

Protein

slyD

PMID:16606699

Experiment(s):EBI-1143817

Protein

yfbU

PMID:16606699

Experiment(s):EBI-1143817
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MALHDENVVW HSHPVTVQQR ELHHGHRGVV LWFTGLSGSG KSTVAGALEE ALHKLGVSTY
LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG LVVLTAFISP HRAERQMVRE
RVGEGRFIEV FVDTPLAICE ARDPKGLYKK ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ
LVTNLVQQLL DLLRQNDIIR S
Length

201

Mol. Wt

22.32 kDa

pI

6.4 (calculated)

Extinction coefficient

15,470 - 15,720 (calc based on 3 Y, 2 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6J1

Domain

27..182

PF01583 Adenylylsulphate kinase

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cysC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130657

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947221

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009023

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6J1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10185

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10185

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947221

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000180

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0182

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

8a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

957

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

3541

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

cysDNC

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2871994..2872034 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2750 (EcoliWiki Page)

NCBI GEO profiles for cysC

microarray

GenExpDB:b2750 (EcoliWiki Page)

Summary of data for cysC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to cysC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10185

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0182

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2750

EcoGene

EcoGene:EG10185

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000180

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009023

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013942 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000013744 (score: 1.000; bootstrap: 100%)
  • ENSAPMP00000016019 (score: 0.604)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G67520 (score: 1.000; bootstrap: 100%)
  • AT4G39940 (score: 0.251)
  • AT2G14750 (score: 0.244)
  • AT3G03900 (score: 0.189)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000017229 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000004152 (score: 0.168)
  • ENSBTAP00000037746 (score: 0.106)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00026273 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00004091 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000016469 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000023016 (score: 0.738)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000018567 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-CDNA-040425-2975 (score: 1.000; bootstrap: 100%)
  • ZDB-GENE-010323-5 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-3477 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0230064 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0020389 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21020-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000017139 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000005834 (score: 0.724)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000265174 (score: 1.000; bootstrap: 100%)
  • ENSP00000354436 (score: 0.741)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000008451 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000026166 (score: 0.632)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000025779 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000020595 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1330587 (score: 1.000; bootstrap: 100%)
  • MGI:1330223 (score: 0.737)

From Inparanoid:20070104

Oryza gramene

  • Q6ZL22 (score: 1.000; bootstrap: 100%)
  • Q9SE92 (score: 0.404)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000044908 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000028084 (score: 0.567)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000014979 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000015077 (score: 0.436)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YKL001C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC17821 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000154154 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000163404 (score: 0.733)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00033455001 (score: 1.000; bootstrap: 100%)
  • GSTENP00024920001 (score: 0.331)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000042964 (score: 1.000; bootstrap: 100%)
  • ENSXETP00000020345 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CYSC

From SHIGELLACYC

E. coli O157

CYSC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01583 Adenylylsulphate kinase

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Panther (EcoliWiki Page)

PTHR11055:SF1

EcoCyc

EcoCyc:EG10185

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10185

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000180

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0182

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009023

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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