cyoD:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cyoD

Mnemonic

Cytochrome o oxidase

Synonyms

ECK0423, b0429, JW0419[1], JW0419

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.63 minutes 

MG1655: 447270..446941
<gbrowseImage> name=NC_000913:446941..447270 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 418749..418420
<gbrowseImage> name=NC_012967:418420..418749 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 349700..350029
<gbrowseImage> name=NC_012759:349700..350029 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 447270..446941
<gbrowseImage> name=NC_007779:446941..447270 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 386601..386272
<gbrowseImage> name=NC_010473:386272..386601 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

cyoD(del) (Keio:JW0419)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC8583[3]

cyoD::Tn5KAN-2 (FB20170)

Insertion at nt 171 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20170

does not contain pKD46

cyoD(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938[5]

cyoD(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[5]

cyoD(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[5]

cyoD786(del)::kan

PMID:16738554[2]

CGSC:100478

cyoD786(del)::FRT

deletion

Mutagenesis Rate

Decrease in Stress Induced Mutagenesis (SIM).

PMID:23224554[6]

Parent Strain: SMR4562 Experimental Strain: SMR12574

The SIM was reduced significantly with a decrease in SIM by 68 to 89 percent. See table S3 for full experimental results.

cyoD786(del)::FRT

deletion

Sensitivity to

UV sensitivity

PMID:23224554[6]

Parent Strain: SMR4562 Experimental Strain: SMR12574

The mutation conferred and increase in UV sensitivity. See table S7 and S1 for a summary of experimental data.

SMR4562 cyoD786(del)::FRTKanFRT yiaG-yfp FRTcatFRT

Deletion & Insertion

Sigma S activity

Decrease in SigmaS activity

PMID:23224554[6]

Parent Strain: SMR10582 Experimental Strain: SMR13035

See table S8 for full experimental results.

CAG45114 cyoD786(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in SigmaE activity

PMID:23224554[6]

Parental Strain: CAG45114 Experimental Strain: SMR15293

See Table S11 for experimental calculations

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0419

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAGTCATTCTACCGATCACAG

Primer 2:CCGTGCATCATCATGTTGTAGTT

2H5

Kohara Phage

Genobase

PMID:3038334[8]

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[3]

est. P1 cotransduction: 58% [9]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 65% [9]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10181

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10181

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000176

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944918

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0178

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001487

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. 5.0 5.1 5.2 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. 6.0 6.1 6.2 6.3 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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