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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	cusF
Gene Synonym(s)	ECK0565, b0573, JW0562, cusX, ylcC^[1], silF, ylcC
Product Desc.	periplasmic copper-binding protein^[2]^[3]; Component of Copper Transporting Efflux System^[3] Silver and copper efflux, periplasmic binding protein; metallochaperone; confers copper and silver resistance; CusF binds both Cu(I) and Ag(I) with high affinity, but does not bind Cu(II)^[4]
Product Synonyms(s)	periplasmic copper-binding protein^[1], B0573^[2]^[1], CusX^[2]^[1], CusF^[2]^[1], YlcC^[2]^[1], ORF110^[2]^[1], agrF^[2]^[1] , cusX, ECK0565, JW0562, silF, ylcC, b0573
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cusCFBA^[2], ylcBCD-ybdE
Regulation/Activity
Quick Links
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Notes

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cusF is in the cusCFBA Cu/Ag efflux operon regulated by Cu and Ag. The first 22 aa are predicted to be a type I signal peptide. However, N-terminal sequencing revealed cleavage after residue 21; the CusF clone construct had eight additional amino acids added at the N-terminus (Fanake, 2003). The cleavage specificity may have been altered by the additional amino acids or the prediction is incorrect.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cusF
Mnemonic	Cu sensor
Synonyms	ECK0565, b0573, JW0562, cusX, ylcC^[1], silF, ylcC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	12.85 minutes	MG1655: 596354..596686 <gbrowseImage> name=NC_000913:596354..596686 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 578034..578366 <gbrowseImage> name=NC_012967:578034..578366 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 499114..499446 <gbrowseImage> name=NC_012759:499114..499446 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 596354..596686 <gbrowseImage> name=NC_007779:596354..596686 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 535686..536018 <gbrowseImage> name=NC_010473:535686..536018 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 648946..649278 <gbrowseImage> name=NC_010473:648946..649278 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	596417	Edman degradation	PMID:12813074
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
cusFM71I		M71I	Loss of copper resistance and strong decrease in copper binding; when associated with I-69			seeded from UniProt:P77214
cusFF73Y		F73Y	No change in copper resistance			seeded from UniProt:P77214
cusFHH57QQ		HH57QQ	Slight decrease in copper resistance			seeded from UniProt:P77214
cusFM69I		M69I	Loss of copper resistance and strong decrease in copper binding; when associated with I-71			seeded from UniProt:P77214
cusFHHH25QQQ		HHH25QQQ	No change in copper resistance			seeded from UniProt:P77214
cusFD48N		D48N	No change in copper resistance			seeded from UniProt:P77214
ΔcusF (Keio:JW0562)	deletion	deletion		PMID:16738554	Shigen CGSC8687^[5]
ΔcusF782::kan				PMID:16738554	CGSC:100444
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0562	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAAAAGCACTGCAAGTCGC Primer 2:CCCTGGCTGACTTTAATATCCTG
2F5	Kohara Phage	Genobase	PMID:3038334
21A9S	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 14% ^[6] Synonyms:purE79::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 19% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6321	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14234	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002929	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945188	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3985	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001967	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CusF
Synonyms	periplasmic copper-binding protein^[1], B0573^[2]^[1], CusX^[2]^[1], CusF^[2]^[1], YlcC^[2]^[1], ORF110^[2]^[1], agrF^[2]^[1] , cusX, ECK0565, JW0562, silF, ylcC, b0573
Product description	periplasmic copper-binding protein^[2]^[3]; Component of Copper Transporting Efflux System^[3] Silver and copper efflux, periplasmic binding protein; metallochaperone; confers copper and silver resistance; CusF binds both Cu(I) and Ag(I) with high affinity, but does not bind Cu(II)^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005507	copper ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:12813074	IMP: Inferred from Mutant Phenotype		C		complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005507	copper ion binding	PMID:12813074	IDA: Inferred from Direct Assay		F		complete
	GO:0016530	metallochaperone activity	PMID:18847219	IDA: Inferred from Direct Assay		F		complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Copper Transporting Efflux System	Y2H- PMID:12813074
Protein	ydiI		PMID:16606699	Experiment(s):EBI-1137215
Protein	ybaA		PMID:16606699	Experiment(s):EBI-1137215
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:12813074	EchoLocation:cusF
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKKALQVAMF SLFTVIGFNA QANEHHHETM SEAQPQVISA TGVVKGIDLE SKKITIHHDP IAAVNWPEMT MRFTITPQTK MSEIKTGDKV AFNFVQQGNL SLLQDIKVSQ
Length	110
Mol. Wt	12.25 kDa
pI	7.8 (calculated)
Extinction coefficient	5,500 (calc based on 0 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-21	UniProt Manual:Signal Peptides	UniProt:P77214
Domain	42..110	PF11604 Copper binding periplasmic protein CusF		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cusF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128556	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945188	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001967	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77214	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6321	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14234	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945188	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002929	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3985	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	48	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	44	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	9^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cusCFBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:596334..596374 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0573 (EcoliWiki Page)	NCBI GEO profiles for cusF
microarray	GenExpDB:b0573 (EcoliWiki Page)	Summary of data for cusF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to cusF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6321	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3985	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0573
EcoGene	EcoGene:EG14234	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002929	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001967	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YLCC	From SHIGELLACYC
E. coli O157	YLCC	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF11604 Copper binding periplasmic protein CusF
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

cusF:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cusF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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