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Expression|

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Standard Name	cusC
Gene Synonym(s)	ECK0564, b0572, JW0561, ylcB, ibeB, agrC^[1]^[2], silC
Product Desc.	outer membrane factor of the CusCFBA copper efflux system^[2]^[3]; Component of Copper Transporting Efflux System^[3] Silver and copper efflux, OM lipoprotein component; outer membrane factor (OMF) of a tripartate efflux pump; confers copper and silver resistance; required for invasion of brain microvascular endothelial cells in strain K1^[4]
Product Synonyms(s)	copper/silver efflux system, outer membrane component^[1], B0572^[2]^[1], IbeB^[2]^[1], CusC^[2]^[1], YlcB^[2]^[1], AgrC^[2]^[1] , ECK0564, ibeB, JW0561, silC, ylcB, b0572
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cusCFBA^[2], ylcBCD-ybdE
Regulation/Activity
Quick Links
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Notes

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cusC is in the cusCFBA Cu/Ag efflux operon regulated by Cu and Ag. TolC, MdtQ, MdtP and CusC are paralogs. First 17 aa are a type II signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cusC
Mnemonic	Cu sensor
Synonyms	ECK0564, b0572, JW0561, ylcB, ibeB, agrC^[1]^[2], silC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	12.82 minutes	MG1655: 594823..596196 <gbrowseImage> name=NC_000913:594823..596196 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 576503..577876 <gbrowseImage> name=NC_012967:576503..577876 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 497583..498956 <gbrowseImage> name=NC_012759:497583..498956 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 594823..596196 <gbrowseImage> name=NC_007779:594823..596196 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 534155..535528 <gbrowseImage> name=NC_010473:534155..535528 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 647415..648788 <gbrowseImage> name=NC_010473:647415..648788 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
cusCQ33H		Q33H		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCQN50RS		QN50RS		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCT81A		T81A		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCL102F		L102F		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCG106D		G106D		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCS273P		S273P		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCG457GWQQ		G457GWQQ		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCS167F		S167F		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCR191G		R191G		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCQ232R		Q232R		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCNT117DS		NT117DS		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCL20M		L20M		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCP27S		P27S		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
cusCL6I		L6I		(in strain: K1 / RS218)			Strain variation; seeded from UniProt:P77211
ΔcusC (Keio:JW0561)	deletion	deletion			PMID:16738554	Shigen CGSC8686^[5]
ΔcusC::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔcusC781::kan					PMID:16738554	CGSC:100442
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0561	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTCCTTGTAAACTTCTGCC Primer 2:CCACCGCCACCAAGTGCGGTATA
2F5	Kohara Phage	Genobase	PMID:3038334
21A9S	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 16% ^[6] Synonyms:purE79::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 18% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6320	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14233	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002928	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946288	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3984	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001958	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CusC
Synonyms	copper/silver efflux system, outer membrane component^[1], B0572^[2]^[1], IbeB^[2]^[1], CusC^[2]^[1], YlcB^[2]^[1], AgrC^[2]^[1] , ECK0564, ibeB, JW0561, silC, ylcB, b0572
Product description	outer membrane factor of the CusCFBA copper efflux system^[2]^[3]; Component of Copper Transporting Efflux System^[3] Silver and copper efflux, OM lipoprotein component; outer membrane factor (OMF) of a tripartate efflux pump; confers copper and silver resistance; required for invasion of brain microvascular endothelial cells in strain K1^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046688	response to copper ion	PMID:12813074	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0008289	lipid binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010131	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010131	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Copper Transporting Efflux System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
outer membrane		From EcoCyc^[3]
Outer membrane	PMID:15174130	EchoLocation:cusC
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSPCKLLPFC VALALTGCSL APDYQRPAMP VPQQFSLSQN GLVNAADNYQ NAGWRTFFVD NQVKTLISEA LVNNRDLRMA TLKVQEARAQ YRLTDADRYP QLNGEGSGSW SGNLKGNTAT TREFSTGLNA SFDLDFFGRL KNMSEAERQN YLATEEAQRA VHILLVSNVA QSYFNQQLAY AQLQIAEETL RNYQQSYAFV EKQLLTGSSN VLALEQARGV IESTRSDIAK RQGELAQANN ALQLLLGSYG KLPQAQTVNS DSLQSVKLPA GLSSQILLQR PDIMEAEHAL MAANANIGAA RAAFFPSISL TSGISTASSD LSSLFNASSG MWNFIPKIEI PIFNAGRNQA NLDIAEIRQQ QSVVNYEQKI QNAFKEVADA LALRQSLNDQ ISAQQRYLAS LQITLQRARA LYQHGAVSYL EVLDAERSLF ATRQTLLDLN YARQVNEISL YTALGGG
Length	457
Mol. Wt	50.27 kDa
pI	6.2 (calculated)
Extinction coefficient	40,340 - 40,715 (calc based on 16 Y, 3 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-17	UniProt Manual:Signal Peptides	UniProt:P77211
Domain	63..247	PF02321 Outer membrane efflux protein		PMID:19920124
Domain	270..455	PF02321 Outer membrane efflux protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cusC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128555	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946288	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001958	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77211	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6320	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14233	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946288	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002928	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3984	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	5	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cusCFBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:594803..594843 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0572 (EcoliWiki Page)	NCBI GEO profiles for cusC
microarray	GenExpDB:b0572 (EcoliWiki Page)	Summary of data for cusC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (594571..594859) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ14; Well:A6^[7]
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Notes

Accessions Related to cusC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6320	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3984	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0572
EcoGene	EcoGene:EG14233	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002928	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001958	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000028688 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YLCB	From SHIGELLACYC
E. coli O157	YLCB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02321 Outer membrane efflux protein
Pfam (EcoliWiki Page)	PF02321 Outer membrane efflux protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:56954
EcoCyc	EcoCyc:G6320	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14233	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002928	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3984	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001958	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cusC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cusC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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