cspE:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
cspE |
---|---|
Gene Synonym(s) |
ECK0616, b0623, JW0618, gicA, msmC[1], msmC |
Product Desc. |
cold shock protein CspE[2]; transcription antiterminator and regulator of RNA stability[3] Binds and melts RNA, involved in cold acclimation; binds ssDNA; high copy protects the chromosome from decondensation by camphor and increases DNA supercoiling; dimeric[4] |
Product Synonyms(s) |
DNA-binding transcriptional repressor[1], B0623[2][1], MsmC[2][1], GicA[2][1], CspE[2][1] , ECK0616, gicA, JW0618, msmC, b0623 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
The cspABEG deletion BX04 is cold-sensitive and forms filaments at 15C (Xia, 2001). CspE is a cold shock protein homolog, but is constitutively expressed at 37C. CspE has transcription antitermination activity. CspE affects rpoS and uspA expression. CspE is a negative regulator of cspA transcription. CspE binds ssDNA containing six or more contiguous dT residues with high affinity (Johnston, 2006).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
cspE |
---|---|
Mnemonic |
Cold shock protein |
Synonyms |
ECK0616, b0623, JW0618, gicA, msmC[1], msmC |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
14.15 minutes |
MG1655: 656515..656724 |
||
NC_012967: 639873..640082 |
||||
NC_012759: 559275..559484 |
||||
W3110 |
|
W3110: 657714..657923 |
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DH10B: 595847..596056 |
||||
DH10B: 709107..709316 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
656518 |
Edman degradation |
PMID:9735283 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔcspE (Keio:JW0618) |
deletion |
deletion |
PMID:16738554 |
| |||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0618 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCTCTAAGATTAAAGGTAACGT Primer 2:CCCAGAGCGATTACGTTTGCAGC | |
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 93% [6] | ||
Linked marker |
est. P1 cotransduction: 14% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002072 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB2096 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002142 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
CspE |
---|---|
Synonyms |
DNA-binding transcriptional repressor[1], B0623[2][1], MsmC[2][1], GicA[2][1], CspE[2][1] , ECK0616, gicA, JW0618, msmC, b0623 |
Product description |
cold shock protein CspE[2]; transcription antiterminator and regulator of RNA stability[3] Binds and melts RNA, involved in cold acclimation; binds ssDNA; high copy protects the chromosome from decondensation by camphor and increases DNA supercoiling; dimeric[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0804 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002059 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003723 |
RNA binding |
PMID:10476034 |
IDA: Inferred from Direct Assay |
F |
Figure 2 |
complete | ||
GO:0003697 |
single-stranded DNA binding |
PMID:17088256 |
IDA: Inferred from Direct Assay |
F |
Figure 1 |
complete | ||
GO:0001072 |
RNA binding transcription antitermination factor activity |
PMID:10884409 |
IDA: Inferred from Direct Assay |
F |
Figure 1, data not shown to determine RNA interaction (HPLC size-exclusion chromatography, native PAGE, and Ni2+-nitrilotriacetic acid agarose coimmobilization assays) |
complete | ||
GO:0060567 |
negative regulation of transcription termination |
PMID:10884409 |
IDA: Inferred from Direct Assay |
P |
complete | |||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
dnaN |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
gabT |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
hflB |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
hrpA |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
pnp |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rho |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rplA |
PMID:15690043 |
Experiment(s):EBI-879589, EBI-883848 | |
Protein |
rplB |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rplC |
PMID:15690043 |
Experiment(s):EBI-879589, EBI-883848 | |
Protein |
rpsA |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rpsD |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rpsG |
PMID:15690043 |
Experiment(s):EBI-879589, EBI-883848 | |
Protein |
secA |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rnr |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
ycbY |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
rluB |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
dcuR |
PMID:15690043 |
Experiment(s):EBI-879589 | |
Protein |
cspI |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
glmU |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
hfq |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
hupA |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
hyfG |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rplD |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rplS |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rplX |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rpsE |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rpsJ |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rpsP |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
serS |
PMID:15690043 |
Experiment(s):EBI-883848 | |
Protein |
rplB |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rpsG |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rpoC |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rpsI |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
ogrK |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rplO |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rplD |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
ygbA |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
yafO |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rpoB |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
hchA |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rplA |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
uspG |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
torA |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
prs |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rplU |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
ulaF |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
rpsD |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
nrdI |
PMID:16606699 |
Experiment(s):EBI-1137461 | |
Protein |
dnaN |
PMID:19402753 |
MALDI(Z-score):38.397986 | |
Protein |
rho |
PMID:19402753 |
MALDI(Z-score):38.327248 | |
Protein |
rpsP |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsJ |
PMID:19402753 |
LCMS(ID Probability):99.0 MALDI(Z-score):3.633502 | |
Protein |
hupA |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
clpA |
PMID:19402753 |
MALDI(Z-score):21.714935 | |
Protein |
rpsA |
PMID:19402753 |
MALDI(Z-score):26.544566 | |
Protein |
rplB |
PMID:19402753 |
MALDI(Z-score):21.191895 | |
Protein |
rpsD |
PMID:19402753 |
MALDI(Z-score):35.999379 | |
Protein |
rplA |
PMID:19402753 |
LCMS(ID Probability):99.0 MALDI(Z-score):24.553794 | |
Protein |
pnp |
PMID:19402753 |
MALDI(Z-score):26.191468 | |
Protein |
hrpA |
PMID:19402753 |
MALDI(Z-score):18.161567 | |
Protein |
glmU |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rnr |
PMID:19402753 |
MALDI(Z-score):25.704700 | |
Protein |
dcuR |
PMID:19402753 |
MALDI(Z-score):17.053160 | |
Protein |
rplD |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):4.393096 | |
Protein |
cspI |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
deaD |
PMID:19402753 |
MALDI(Z-score):27.600158 | |
Protein |
hfq |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rluB |
PMID:19402753 |
LCMS(ID Probability):90.0 MALDI(Z-score):33.056271 | |
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Notes
selective RNA binding sequence
AAAUUU
PMID: 10476034
Figure 1
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MSKIKGNVKW FNESKGFGFI TPEDGSKDVF VHFSAIQTNG FKTLAEGQRV EFEITNGAKG PSAANVIAL |
Length |
69 |
Mol. Wt |
7.463 kDa |
pI |
9.6 (calculated) |
Extinction coefficient |
5,500 (calc based on 0 Y, 1 W, and 0 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0002142 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120002072 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2096 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 strain JC7623 |
30 uM |
uM |
|
Estimated from 2D-PAGE, data not shown. |
PMID:10200963 | |
Protein |
E. coli K-12 MC4100 |
8.19E+03 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
3057.05+/-24.364 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
1.8759+/-0.19292 |
Molecules/cell |
|
by FISH |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
5.224880383 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
186416 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
29309 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
56258 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:656495..656535
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for cspE | |
microarray |
Summary of data for cspE from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to cspE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2096 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002072 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002142 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Anopheles gambiae |
|
From Inparanoid:20070104 |
Apis mellifera |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Bos taurus |
|
From Inparanoid:20070104 |
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
Canis familiaris |
|
From Inparanoid:20070104 |
Ciona intestinalis |
|
From Inparanoid:20070104 |
Danio rerio |
|
From Inparanoid:20070104 |
Drosophila melanogaster |
|
From Inparanoid:20070104 |
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Macaca mulatta |
|
From Inparanoid:20070104 |
Monodelphis domestica |
|
From Inparanoid:20070104 |
Mus musculus |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Pan troglodytes |
|
From Inparanoid:20070104 |
Rattus norvegicus |
|
From Inparanoid:20070104 |
Takifugu rubripes |
|
From Inparanoid:20070104 |
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
CSPE |
From SHIGELLACYC |
E. coli O157 |
CSPE |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12179 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002072 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2096 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002142 |
Escherichia coli str. K-12 substr. MG1655 | |
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<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
Categories
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Danio rerio
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Oryza gramene
- Genes with homologs in Pan troglodytes
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157