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Product(s) |

Expression|

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Standard Name	chbF
Gene Synonym(s)	ECK1732, b1734, JW1723, celF, ydjD^[1], ydjD
Product Desc.	6-phospho-β-glucosidase; cryptic^[2]^[3] N,N'-diacetylchitobiose utilization, phospho-chitobiase; can hydrolyze a variety of phospho-beta-glucosides^[4]
Product Synonyms(s)	cryptic phospho-beta-glucosidase, NAD(P)-binding^[1], B1734^[2]^[1], YdjD^[2]^[1], ChbF^[2]^[1], CelF^[2]^[1], phospho-β-glucosidase; cryptic^[2]^[1] , celF, ECK1732, JW1723, ydjD, b1734
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): chbBCARFG^[2]
Regulation/Activity
Quick Links
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Notes

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The chb operon is induced by N,N'-diacetylchitobiose. The chb operon was previously thought to encode a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent, but a fully induced operon did not support growth on cellobiose, implying a mutation altering the substrate specificity is needed; several candidate mutations were noted in Genbank, but not proven to alter the specificity of the system (Keyhani, 1997). In contrast, a later study claim that mutations making the operon expression high-level constitutive by chbR and nagC mutations are sufficient for the Cel+ phenotype and that no mutations altering substrate specificity are required (Kachroo, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	chbF
Mnemonic	Chitobiose
Synonyms	ECK1732, b1734, JW1723, celF, ydjD^[1], ydjD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	39.12 minutes	MG1655: 1816524..1815172 <gbrowseImage> name=NC_000913:1815172..1816524 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1794413..1795765 <gbrowseImage> name=NC_012967:1794413..1795765 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1707231..1708583 <gbrowseImage> name=NC_012759:1707231..1708583 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1820214..1818862 <gbrowseImage> name=NC_007779:1818862..1820214 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1907095..1905743 <gbrowseImage> name=NC_010473:1905743..1907095 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1815175	Edman degradation	PMID:10572139
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔchbF (Keio:JW1723)	deletion	deletion	PMID:16738554	Shigen CGSC9455^[5]
celF::Tn5KAN-2 (FB20441)	Insertion at nt 1164 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20441	contains pKD46
ΔchbF733::kan			PMID:16738554	CGSC:100290
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1723	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCCAGAAATTAAAAGTCGT Primer 2:CCATGTGCTTTTTTAAGCTCTGC
19B6	Kohara Phage	Genobase	PMID:3038334
4F7	Kohara Phage	Genobase	PMID:3038334
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 20% ^[6] Synonyms:zdh-925::Tn10
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 53% ^[6] Synonyms:zdi-276::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10144	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10144	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000140	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946266	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0142	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005785	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ChbF
Synonyms	cryptic phospho-beta-glucosidase, NAD(P)-binding^[1], B1734^[2]^[1], YdjD^[2]^[1], ChbF^[2]^[1], CelF^[2]^[1], phospho-β-glucosidase; cryptic^[2]^[1] , celF, ECK1732, JW1723, ydjD, b1734
Product description	6-phospho-β-glucosidase; cryptic^[2]^[3] N,N'-diacetylchitobiose utilization, phospho-chitobiase; can hydrolyze a variety of phospho-beta-glucosides^[4]
EC number (for enzymes)	3.2.1.86^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015955	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001088	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019802	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005488	binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008706	6-phospho-beta-glucosidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.2.1.86	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016151	nickel ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0533	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016798	hydrolase activity, acting on glycosyl bonds	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0464	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050897	cobalt ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0170	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI IFDLCQRMID NAGVPMKLYK TLDRREALKD ADFVTTQLRV GQLPARELDE RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC PNAWVINFTN PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG LNHMVFIKDV LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG EFNDVLLALN LSPLVHSDRD AELLAREMIL AHEKWLPNFA DCIAELKKAH
Length	450
Mol. Wt	50.514 kDa
pI	6.2 (calculated)
Extinction coefficient	44,350 - 45,225 (calc based on 15 Y, 4 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P17411
Domain	6..188	PF02056 Family 4 glycosyl hydrolase		PMID:19920124
Domain	192..415	PF11975 Family 4 glycosyl hydrolase C-terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=chbF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129688	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946266	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005785	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P17411	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10144	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10144	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946266	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000140	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0142	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	20	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	10	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	15^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
chbBCARFG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1816504..1816544 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1734 (EcoliWiki Page)	NCBI GEO profiles for chbF
microarray	GenExpDB:b1734 (EcoliWiki Page)	Summary of data for chbF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to chbF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10144	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0142	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1734
EcoGene	EcoGene:EG10144	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000140	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005785	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	CELF	From SHIGELLACYC
E. coli O157	CELF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02056 Family 4 glycosyl hydrolase
Superfamily (EcoliWiki Page)	SUPERFAMILY:51735
Superfamily (EcoliWiki Page)	SUPERFAMILY:56327
Pfam (EcoliWiki Page)	PF11975 Family 4 glycosyl hydrolase C-terminal domain
EcoCyc	EcoCyc:EG10144	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10144	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000140	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0142	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005785	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

chbF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to chbF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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