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Standard Name	brnQ
Gene Synonym(s)	ECK0395, b0401, JW0391, hrbA^[1], hrbA
Product Desc.	BrnQ branched chain amino acid transporter^[2]^[3] Mutants are valine and o-methylthreonine resistant, glycylvaline sensitive; transport system I for Ile, Leu, and Val^[4]
Product Synonyms(s)	predicted branched chain amino acid transporter (LIV-II)^[1], B0401^[2]^[1], HrbA^[2]^[1], LIV-II^[2]^[1], BrnQ^[2]^[1] , ECK0395, hrbA, JW0391, b0401
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): brnQ^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	brnQ
Mnemonic	Branched chain
Synonyms	ECK0395, b0401, JW0391, hrbA^[1], hrbA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	9.03 minutes	MG1655: 418815..420134 <gbrowseImage> name=NC_000913:418815..420134 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 388014..389333 <gbrowseImage> name=NC_012967:388014..389333 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 321574..322893 <gbrowseImage> name=NC_012759:321574..322893 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 418815..420134 <gbrowseImage> name=NC_007779:418815..420134 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 358146..359465 <gbrowseImage> name=NC_010473:358146..359465 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔbrnQ (Keio:JW0391)	deletion	deletion	PMID:16738554	Shigen CGSC8567^[5]
brnQ4				CGSC:6901
brnQ2				CGSC:14639
ΔbrnQ765::kan			PMID:16738554	CGSC:100210
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0391	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACCCATCAATTAAGATCGCG Primer 2:CCGTGAGCGCTGGAGGTCACCTG
9G9	Kohara Phage	Genobase	PMID:3038334
zai-3053::Tn10	Linked marker	CAG18091 = CGSC7336^[5]	est. P1 cotransduction: 40% ^[6] Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 64% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12168	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12168	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002064	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945042	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2087	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001394	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BrnQ
Synonyms	predicted branched chain amino acid transporter (LIV-II)^[1], B0401^[2]^[1], HrbA^[2]^[1], LIV-II^[2]^[1], BrnQ^[2]^[1] , ECK0395, hrbA, JW0391, b0401
Product description	BrnQ branched chain amino acid transporter^[2]^[3] Mutants are valine and o-methylthreonine resistant, glycylvaline sensitive; transport system I for Ile, Leu, and Val^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015658	branched-chain aliphatic amino acid transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004685	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015803	branched-chain aliphatic amino acid transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004685	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015820	leucine transport	PMID:4590465	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004685	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015803	branched-chain aliphatic amino acid transport	PMID:4590465	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0015829	valine transport	PMID:4590465	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0015818	isoleucine transport	PMID:4590465	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0015820	leucine transport	PMID:4590465	IMP: Inferred from Mutant Phenotype		P	inhibited by threonine	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTHQLRSRDI IALGFMTFAL FVGAGNIIFP PMVGLQAGEH VWTAAFGFLI TAVGLPVLTV VALAKVGGGV DSLSTPIGKV AGVLLATVCY LAVGPLFATP RTATVSFEVG IAPLTGDSAL PLFIYSLVYF AIVILVSLYP GKLLDTVGNF LAPLKIIALV ILSVAAIVWP AGSISTATEA YQNAAFSNGF VNGYLTMDTL GAMVFGIVIV NAARSRGVTE ARLLTRYTVW AGLMAGVGLT LLYLALFRLG SDSASLVDQS ANGAAILHAY VQHTFGGGGS FLLAALIFIA CLVTAVGLTC ACAEFFAQYV PLSYRTLVFI LGGFSMVVSN LGLSQLIQIS VPVLTAIYPP CIALVVLSFT RSWWHNSSRV IAPPMFISLL FGILDGIKAS AFSDILPSWA QRLPLAEQGL AWLMPTVVMV VLAIIWDRAA GRQVTSSAH
Length	439
Mol. Wt	46.209 kDa
pI	8.4 (calculated)
Extinction coefficient	61,880 - 62,505 (calc based on 12 Y, 8 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	6..430	PF05525 Branched-chain amino acid transport protein		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=brnQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128386	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945042	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001394	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AD99	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12168	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12168	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945042	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002064	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2087	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	3561	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	167	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	713	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
brnQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:418795..418835 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0401 (EcoliWiki Page)	NCBI GEO profiles for brnQ
microarray	GenExpDB:b0401 (EcoliWiki Page)	Summary of data for brnQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (418312..418864) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ12; Well:G8^[8]
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Notes

Accessions Related to brnQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12168	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2087	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0401
EcoGene	EcoGene:EG12168	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002064	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001394	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000028600 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	BRNQ	From SHIGELLACYC
E. coli O157	BRNQ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF05525 Branched-chain amino acid transport protein
EcoCyc	EcoCyc:EG12168	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12168	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002064	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2087	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001394	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

brnQ:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to brnQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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