brnQ:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

BrnQ

Synonyms

predicted branched chain amino acid transporter (LIV-II)[1], B0401[2][1], HrbA[2][1], LIV-II[2][1], BrnQ[2][1] , ECK0395, hrbA, JW0391, b0401

Product description

BrnQ branched chain amino acid transporter[2][3]

Mutants are valine and o-methylthreonine resistant, glycylvaline sensitive; transport system I for Ile, Leu, and Val[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0029

P

Seeded from EcoCyc (v14.0)

complete

GO:0015658

branched-chain aliphatic amino acid transmembrane transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004685

F

Seeded from EcoCyc (v14.0)

complete

GO:0015803

branched-chain aliphatic amino acid transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004685

P

Seeded from EcoCyc (v14.0)

complete

GO:0015820

leucine transport

PMID:4590465[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004685

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0015803

branched-chain aliphatic amino acid transport

PMID:4590465[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015829

valine transport

PMID:4590465[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015818

isoleucine transport

PMID:4590465[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015820

leucine transport

PMID:4590465[5]

IMP: Inferred from Mutant Phenotype

P

inhibited by threonine

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [6]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTHQLRSRDI IALGFMTFAL FVGAGNIIFP PMVGLQAGEH VWTAAFGFLI TAVGLPVLTV
VALAKVGGGV DSLSTPIGKV AGVLLATVCY LAVGPLFATP RTATVSFEVG IAPLTGDSAL
PLFIYSLVYF AIVILVSLYP GKLLDTVGNF LAPLKIIALV ILSVAAIVWP AGSISTATEA
YQNAAFSNGF VNGYLTMDTL GAMVFGIVIV NAARSRGVTE ARLLTRYTVW AGLMAGVGLT
LLYLALFRLG SDSASLVDQS ANGAAILHAY VQHTFGGGGS FLLAALIFIA CLVTAVGLTC
ACAEFFAQYV PLSYRTLVFI LGGFSMVVSN LGLSQLIQIS VPVLTAIYPP CIALVVLSFT
RSWWHNSSRV IAPPMFISLL FGILDGIKAS AFSDILPSWA QRLPLAEQGL AWLMPTVVMV
VLAIIWDRAA GRQVTSSAH
Length

439

Mol. Wt

46.209 kDa

pI

8.4 (calculated)

Extinction coefficient

61,880 - 62,505 (calc based on 12 Y, 8 W, and 5 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..430

PF05525 Branched-chain amino acid transport protein

PMID:19920124[7]

<motif_map/>

tmhmm.php?gene=brnQ&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=brnQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128386

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945042

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001394

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AD99

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12168

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12168

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945042

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002064

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2087

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 Guardiola, J et al. (1974) Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J. Bacteriol. 117 393-405 PubMed
  6. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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