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Standard Name	bolA
Gene Synonym(s)	ECK0429, b0435, JW5060^[1], JW5060
Product Desc.	BolA transcriptional regulator^[2]; BolA transcriptional dual regulator^[3] Transcriptional repressor for mreB, morphogene; shape determinant; overexpression produces osmotically stable spherical cells; FtsZ dependent; induced by AI-2 pheromone^[4]
Product Synonyms(s)	regulator of penicillin binding proteins and beta lactamase transcription (morphogene)^[1], B0435^[2]^[1], BolA^[2]^[1] , ECK0429, JW5060, b0435
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): bolA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	bolA
Mnemonic	Bolus
Synonyms	ECK0429, b0435, JW5060^[1], JW5060
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	9.78 minutes	MG1655: 453696..454013 <gbrowseImage> name=NC_000913:453696..454013 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 425177..425494 <gbrowseImage> name=NC_012967:425177..425494 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 356455..356772 <gbrowseImage> name=NC_012759:356455..356772 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 453696..454013 <gbrowseImage> name=NC_007779:453696..454013 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 393027..393344 <gbrowseImage> name=NC_010473:393027..393344 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
bolA(del) (Keio:JW5060)	deletion	deletion			PMID:16738554	Shigen
bolA2::kan					PMID:2684651	CGSC:19396
overexpression of bolA			Cell Shape	round instead of rod-shaped cells	PMID:3053647
bolA⁺			overexpression	5-6 fold increase in expression under oxidative stress in the planktonic phase when compared with rpoS expression, fig 7.	PMID:21630090
bolA⁺			overexpression	2.2-fold incrase in the expression of bolA were seen after heat stress under biofilm and planktonic stages of growth, fig 7.	PMID:21630090
bolA			expression	Variation in the pH change induces the expression of rpoS up to 3.5-5.5 fold increase under biofilm mode of growth, figure 7.	PMID:21630090
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5060	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGATACGTGAGCGGATAGA Primer 2:CCtGCGATGCTTCCTGCTCCACG
2H5	Kohara Phage	Genobase	PMID:3038334
3B6	Kohara Phage	Genobase	PMID:3038334
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 44% ^[6]
zaj-3054::Tn10	Linked marker	CAG12017 = CGSC7339^[5]	est. P1 cotransduction: 83% ^[6] Synonyms:zba-3054::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10125	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10125	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000121	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947043	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0123	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001508	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BolA
Synonyms	regulator of penicillin binding proteins and beta lactamase transcription (morphogene)^[1], B0435^[2]^[1], BolA^[2]^[1] , ECK0429, JW5060, b0435
Product description	BolA transcriptional regulator^[2]; BolA transcriptional dual regulator^[3] Transcriptional repressor for mreB, morphogene; shape determinant; overexpression produces osmotically stable spherical cells; FtsZ dependent; induced by AI-2 pheromone^[4]
EC number (for enzymes)
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Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	grxD	PMID:15690043	Experiment(s):EBI-889216, EBI-893481
Protein	yleA	PMID:15690043	Experiment(s):EBI-889216
Protein	dppB	PMID:15690043	Experiment(s):EBI-894289
Protein	yehY	PMID:15690043	Experiment(s):EBI-894289
Protein	miaB	PMID:19402753	MALDI(Z-score):24.026932
Protein	dppB	PMID:19402753	LCMS(ID Probability):99.0
Protein	yehY	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTSQRCRRRY FMMIRERIEE KLRAAFQPVF LEVVDESYRH NVPAGSESHF KVVLVSDRFT GERFLNRHRM IYSTLAEELS TTVHALALHT YTIKEWEGLQ DTVFASPPCR GAGSIA
Length	116
Mol. Wt	13.478 kDa
pI	8.7 (calculated)
Extinction coefficient	11,460 - 11,710 (calc based on 4 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	9..85	PF01722 BolA-like protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=bolA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111135	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947043	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001508	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABE2	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10125	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10125	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947043	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000121	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0123	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	7.04E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	82.072+/-0.693	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.23293+/-0.06208	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.444794953	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1945	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2040	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1147	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
bolA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:453676..453716 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0435 (EcoliWiki Page)	NCBI GEO profiles for bolA
microarray	GenExpDB:b0435 (EcoliWiki Page)	Summary of data for bolA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (453423..453723) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:D9^[7]
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Notes

Accessions Related to bolA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10125	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0123	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0435
EcoGene	EcoGene:EG10125	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000121	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001508	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000021299 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000017347 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G55805 (score: 1.000; bootstrap: 57%) AT4G26500 (score: 1.000; bootstrap: 57%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00040085 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00019658 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000017008 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040801-76 (score: 1.000; bootstrap: 98%) ZDB-CDNA-040801-83 (score: 1.000; bootstrap: 98%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0188836 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0051126 (score: 1.000; bootstrap: 81%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA16028-PA (score: 1.000; bootstrap: 81%)	From Inparanoid:20070104
Homo sapiens	ENSP00000324778 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000009517 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000023383 (score: 1.000; bootstrap: 94%)	From Inparanoid:20070104
Mus musculus	MGI:1916418 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000002104 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000028767 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC16E96c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000180585 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00019514001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000024152 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Shigella flexneri	BOLA	From SHIGELLACYC
E. coli O157	BOLA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01722 BolA-like protein
Panther (EcoliWiki Page)	PTHR12735:SF3
Superfamily (EcoliWiki Page)	SUPERFAMILY:82657
EcoCyc	EcoCyc:EG10125	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10125	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000121	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0123	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001508	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

bolA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to bolA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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