atpE:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

atpE

Mnemonic

ATP

Synonyms

ECK3730, b3737, JW3715, papH, uncE[1], uncE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.47 minutes 

MG1655: 3919212..3918973
<gbrowseImage> name=NC_000913:3918973..3919212 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3881555..3881316
<gbrowseImage> name=NC_012967:3881316..3881555 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3807306..3807545
<gbrowseImage> name=NC_012759:3807306..3807545 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3715492..3715731
<gbrowseImage> name=NC_007779:3715492..3715731 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4016796..4016557
<gbrowseImage> name=NC_010473:4016557..4016796 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3918973

Edman degradation

PMID:6256167[2]
PMID:6446460[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

atpEL31F

L31F

In uncE408 and uncE463; unable to assemble in the membrane

seeded from UniProt:P68699

atpEI28V

I28V

In DC1; has a functional F0 as well as F1 part. However, the ATPase activity is inhibited

seeded from UniProt:P68699

atpEG23D

G23D

In uncE429; unable to assemble in the membrane

seeded from UniProt:P68699

ΔatpE (Keio:JW3715)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10716[5]

atpED61G

D61G

In DG 7/1; contains an enzymatically active F1 component, but no functional F0 component

seeded from UniProt:P68699

atpE::Tn5KAN-I-SceI (FB21426)

Insertion at nt 185 in Plus orientation

PMID:15262929[6]

E. coli Genome Project:FB21426

contains pKD46

atpE429

CGSC:7166

ΔatpE766::kan

PMID:16738554[4]

CGSC:100142


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3715

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGAAAACCTGAATATGGATCT

Primer 2:CCgGCGACAGCGAACATCACGTA

2A1

Kohara Phage

Genobase

PMID:3038334[8]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 14% [9]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[5]

est. P1 cotransduction: 69% [9]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10102

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10102

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000098

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948253

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0100

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012220

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Hoppe, J et al. (1980) Identification of amino-acid substitutions in the proteolipid subunit of the ATP synthase from dicyclohexylcarbodiimide-resistant mutants of Escherichia coli. Eur. J. Biochem. 112 17-24 PubMed
  3. Wachter, E et al. (1980) Amino acid replacement in dicyclohexylcarbodiimide-reactive proteins from mutant strains of Escherichia coli defective in the energy-transducing ATPase complex. FEBS Lett. 113 265-70 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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