aspA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

aspA

Gene Synonym(s)

ECK4133, b4139, JW4099[1], JW4099

Product Desc.

AspA[2][3];

Component of aspartate ammonia-lyase[2][3]

L-Aspartate ammonia-lyase; L-aspartase[4]

Product Synonyms(s)

aspartate ammonia-lyase[1], B4139[2][1], AspA[2][1] , ECK4133, JW4099, b4139

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): aspA-dcuA[2], OP00219, aspA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Tetrameric enzyme. Repressed by nitrate via NarXL mediated repression.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aspA

Mnemonic

Aspartate

Synonyms

ECK4133, b4139, JW4099[1], JW4099

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.08 minutes, 94.08 minutes 

MG1655: 4366350..4364914
<gbrowseImage> name=NC_000913:4364914..4366350 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4347048..4345612
<gbrowseImage> name=NC_012967:4345612..4347048 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4303649..4305085
<gbrowseImage> name=NC_012759:4303649..4305085 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4373007..4371571
<gbrowseImage> name=NC_007779:4371571..4373007 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4466712..4465276
<gbrowseImage> name=NC_010473:4465276..4466712 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4364917

Edman degradation

PMID:2987841


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

aspAK327R

K327R

Auxotrophies

Reduces activity by 99.7%. Increases KM for aspartic acid 5-fold

seeded from UniProt:P0AC38

aspAR29A

R29A

Auxotrophies

No effect on Kcat. Increases KM for aspartic acid 40-fold

seeded from UniProt:P0AC38

aspAK55R

K55R

Auxotrophies

Loss of activity

seeded from UniProt:P0AC38

aspAH124L

H124L

Auxotrophies

Reduces activity by 30%

seeded from UniProt:P0AC38

aspAS143G

S143G

Auxotrophies

Reduces Kcat by 90%. Increases KM for aspartic acid 4-fold

seeded from UniProt:P0AC38

aspAS143T

S143T

Auxotrophies

Reduces Kcat by 98.5%. Increases KM for aspartic acid 2-fold

seeded from UniProt:P0AC38

aspAD10A

D10A

Auxotrophies

Loss of activity

seeded from UniProt:P0AC38

aspAD10N

D10N

Auxotrophies

Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold

seeded from UniProt:P0AC38

aspAR15A

R15A

Auxotrophies

No effect on Kcat. Increases KM for aspartic acid 2.5-fold

seeded from UniProt:P0AC38

aspAH26N

H26N

Auxotrophies

No effect on Kcat. Increases KM for aspartic acid 3-fold

seeded from UniProt:P0AC38

ΔaspA (Keio:JW4099)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen

CGSC8324[5]

aspA22

Auxotrophies

CGSC:5112

aspA23

Auxotrophies

CGSC:5113

ΔaspA765::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:100110


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4099

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCAAACAACATTCGTATCGA

Primer 2:CCtTGTTCGCTTTCATCAGTATA

5G7

Kohara Phage

Genobase

PMID:3038334

9B1

Kohara Phage

Genobase

PMID:3038334

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 76% [6]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[5]

est. P1 cotransduction: 4% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10095

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10095

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000091

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948658

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0093

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013551

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AspA

Synonyms

aspartate ammonia-lyase[1], B4139[2][1], AspA[2][1] , ECK4133, JW4099, b4139

Product description

AspA[2][3];

Component of aspartate ammonia-lyase[2][3]

L-Aspartate ammonia-lyase; L-aspartase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000362

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008948

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020557

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006099

tricarboxylic acid cycle

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018951

P

Seeded from EcoCyc (v14.0)

complete

GO:0006531

aspartate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004708

P

Seeded from EcoCyc (v14.0)

complete

GO:0008797

aspartate ammonia-lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004708

F

Seeded from EcoCyc (v14.0)

complete

GO:0008797

aspartate ammonia-lyase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.3.1.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018951

F

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0456

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of aspartate ammonia-lyase

could be indirect

Protein

dnaK

PMID:15690043

Experiment(s):EBI-878610, EBI-881328

Protein

secA

PMID:15690043

Experiment(s):EBI-878610

Protein

otsB

PMID:16606699

Experiment(s):EBI-1147527

Protein

chbF

PMID:16606699

Experiment(s):EBI-1147527

Protein

nadE

PMID:16606699

Experiment(s):EBI-1147527

Protein

rhsD

PMID:16606699

Experiment(s):EBI-1147527

Protein

carB

PMID:16606699

Experiment(s):EBI-1147527

Protein

yjbI

PMID:16606699

Experiment(s):EBI-1147527
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MCLKQIIGSL KKKVHMSNNI RIEEDLLGTR EVPADAYYGV HTLRAIENFY ISNNKISDIP
EFVRGMVMVK KAAAMANKEL QTIPKSVANA IIAACDEVLN NGKCMDQFPV DVYQGGAGTS
VNMNTNEVLA NIGLELMGHQ KGEYQYLNPN DHVNKCQSTN DAYPTGFRIA VYSSLIKLVD
AINQLREGFE RKAVEFQDIL KMGRTQLQDA VPMTLGQEFR AFSILLKEEV KNIQRTAELL
LEVNLGATAI GTGLNTPKEY SPLAVKKLAE VTGFPCVPAE DLIEATSDCG AYVMVHGALK
RLAVKMSKIC NDLRLLSSGP RAGLNEINLP ELQAGSSIMP AKVNPVVPEV VNQVCFKVIG
NDTTVTMAAE AGQLQLNVME PVIGQAMFES VHILTNACYN LLEKCINGIT ANKEVCEGYV
YNSIGIVTYL NPFIGHHNGD IVGKICAETG KSVREVVLER GLLTEAELDD IFSVQNLMHP
AYKAKRYTDE SEQ
Length

493

Mol. Wt

54.064 kDa

pI

5.6 (calculated)

Extinction coefficient

23,840 - 25,340 (calc based on 16 Y, 0 W, and 12 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

13..345

PF00206 Lyase

PMID:19920124

Domain

411..465

PF10415 Fumarase C C-terminus

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aspA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111690

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948658

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013551

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AC38

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10095

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10095

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948658

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000091

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0093

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

5.26E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

20.588+/-0.192

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.036211699

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1119

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

71

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

469

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

aspA-dcuA

aspA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4366330..4366370 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4139 (EcoliWiki Page)

NCBI GEO profiles for aspA

microarray

GenExpDB:b4139 (EcoliWiki Page)

Summary of data for aspA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4365857..4366405) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ05; Well:F4[7]

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Notes

Accessions Related to aspA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10095

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0093

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4139

EcoGene

EcoGene:EG10095

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000091

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013551

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

ASPA

From SHIGELLACYC

E. coli O157

ASPA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00206 Lyase

Superfamily (EcoliWiki Page)

SUPERFAMILY:48557

Panther (EcoliWiki Page)

PTHR11444:SF8

Pfam (EcoliWiki Page)

PF10415 Fumarase C C-terminus

EcoCyc

EcoCyc:EG10095

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10095

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000091

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0093

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013551

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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