aspA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
aspA |
|---|---|
| Mnemonic |
Aspartate |
| Synonyms |
ECK4133, b4139, JW4099[1], JW4099 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
94.08 minutes, 94.08 minutes |
MG1655: 4366350..4364914 |
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NC_012967: 4347048..4345612 |
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NC_012759: 4303649..4305085 |
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W3110 |
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W3110: 4373007..4371571 |
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DH10B: 4466712..4465276 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4364917 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
aspAK327R |
K327R |
Auxotrophies |
Reduces activity by 99.7%. Increases KM for aspartic acid 5-fold |
seeded from UniProt:P0AC38 | |||
|
aspAR29A |
R29A |
Auxotrophies |
No effect on Kcat. Increases KM for aspartic acid 40-fold |
seeded from UniProt:P0AC38 | |||
|
aspAK55R |
K55R |
Auxotrophies |
Loss of activity |
seeded from UniProt:P0AC38 | |||
|
aspAH124L |
H124L |
Auxotrophies |
Reduces activity by 30% |
seeded from UniProt:P0AC38 | |||
|
aspAS143G |
S143G |
Auxotrophies |
Reduces Kcat by 90%. Increases KM for aspartic acid 4-fold |
seeded from UniProt:P0AC38 | |||
|
aspAS143T |
S143T |
Auxotrophies |
Reduces Kcat by 98.5%. Increases KM for aspartic acid 2-fold |
seeded from UniProt:P0AC38 | |||
|
aspAD10A |
D10A |
Auxotrophies |
Loss of activity |
seeded from UniProt:P0AC38 | |||
|
aspAD10N |
D10N |
Auxotrophies |
Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold |
seeded from UniProt:P0AC38 | |||
|
aspAR15A |
R15A |
Auxotrophies |
No effect on Kcat. Increases KM for aspartic acid 2.5-fold |
seeded from UniProt:P0AC38 | |||
|
aspAH26N |
H26N |
Auxotrophies |
No effect on Kcat. Increases KM for aspartic acid 3-fold |
seeded from UniProt:P0AC38 | |||
|
ΔaspA (Keio:JW4099) |
deletion |
deletion |
Auxotrophies |
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aspA22 |
Auxotrophies |
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aspA23 |
Auxotrophies |
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ΔaspA765::kan |
deletion |
deletion |
Auxotrophies |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW4099 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCAAACAACATTCGTATCGA Primer 2:CCtTGTTCGCTTTCATCAGTATA | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 76% [7] | ||
|
Linked marker |
est. P1 cotransduction: 4% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Takagi, JS et al. (1985) Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W. Nucleic Acids Res. 13 2063-74 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
