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Standard Name	arsB
Gene Synonym(s)	ECK3487, b3502, JW3469, arsF, f172^[1]^[2]
Product Desc.	ArsB^[2]^[3]; Component of arsenate Ars transporter^[2]^[3] Arsenite pump, inner membrane; resistance to arsenate, arsenite, and antimonite^[4]
Product Synonyms(s)	arsenite/antimonite transporter^[1], B3502^[2]^[1], ArsF^[2]^[1], F172^[2]^[1] , arsF, ECK3487, JW3469, b3502
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): arsRBC^[2]
Regulation/Activity
Quick Links
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Notes

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ArsB can function alone using PMF to drive transport, as is the case with the chromosomal ArsB, which lacks the ArsA ATPase; ArsB proteins can use ATP to drive efflux if ArsA is present. The chromosomal ArsB is listed in TCDB as one of two 3.4.A.1.1 proteins; the TC 3.4.A.1.1 ArsA protein is plasmid-derived. The correct TC class for the stand-alone E. coli chromsomal ArsB is 2.A.45 and the ArsB protein should probably be re-classified as TC 2.A.45.1.2. IT (Ion Transporter) superfamily (Prakash, 2003). ST(3) structural class based on hydropathy profile (Lolkema, 2003).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	arsB
Mnemonic	Arsenate resistance
Synonyms	ECK3487, b3502, JW3469, arsF, f172^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	78.6 minutes	MG1655: 3646958..3648247 <gbrowseImage> name=NC_000913:3646958..3648247 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3579625..3580914 <gbrowseImage> name=NC_012967:3579625..3580914 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3535452..3536741 <gbrowseImage> name=NC_012759:3535452..3536741 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3991480..3990191 <gbrowseImage> name=NC_007779:3990191..3991480 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3744703..3745992 <gbrowseImage> name=NC_010473:3744703..3745992 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔarsB (Keio:JW3469)	deletion	deletion	PMID:16738554	Shigen CGSC10571^[5]
arsB::Tn5KAN-I-SceI (FB21246)	Insertion at nt 267 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21246	contains pKD46
ΔarsB758::kan			PMID:16738554	CGSC:100082
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3469	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTACTGGCAGGCGCTATCTT Primer 2:CCtAAAGTGAAAGAGAGACGTAG
4D9	Kohara Phage	Genobase	PMID:3038334
12E4	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: 6% ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002120	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948011	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2148	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011437	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ArsB
Synonyms	arsenite/antimonite transporter^[1], B3502^[2]^[1], ArsF^[2]^[1], F172^[2]^[1] , arsF, ECK3487, JW3469, b3502
Product description	ArsB^[2]^[3]; Component of arsenate Ars transporter^[2]^[3] Arsenite pump, inner membrane; resistance to arsenate, arsenite, and antimonite^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000802	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046685	response to arsenic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0059	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005887	integral to plasma membrane	PMID:20196076	IDA: Inferred from Direct Assay		C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of arsenate Ars transporter	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized to the periplasm with 11 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFYPGGIMLL AGAIFVLTIV LVIWQPKGLG IGWSATLGAV LALVTGVVHP GDIPVVWNIV WNATAAFIAV IIISLLLDES GFFEWAALHV SRWGNGRGRL LFTWIVLLGA AVAALFANDG AALILTPIVI AMLLALGFSK GTTLAFVMAA GFIADTASLP LIVSNLVNIV SADFFGLGFR EYASVMVPVD IAAIVATLVM LHLYFRKDIP QNYDMALLKS PAEAIKDPAT FKTGWVVLLL LLVGFFVLEP LGIPVSAIAA VGALILFVVA KRGHAINTGK VLRGAPWQIV IFSLGMYLVV YGLRNAGLTE YLSGVLNVLA DNGLWAATLG TGFLTAFLSS IMNNMPTVLV GALSIDGSTA SGVIKEAMVY ANVIGCDLGP KITPIGSLAT LLWLHVLSQK NMTISWGYYF RTGIIMTLPV LFVTLAALAL RLSFTL
Length	436
Mol. Wt	46.264 kDa
pI	8.8 (calculated)
Extinction coefficient	80,900 - 81,025 (calc based on 10 Y, 12 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..425	PF02040 Arsenical pump membrane protein		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=arsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111602	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948011	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011437	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AB93	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12236	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948011	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002120	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2148	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	48	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
arsRBC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3646938..3646978 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3502 (EcoliWiki Page)	NCBI GEO profiles for arsB
microarray	GenExpDB:b3502 (EcoliWiki Page)	Summary of data for arsB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to arsB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12236	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2148	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3502
EcoGene	EcoGene:EG12236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002120	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011437	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000012974 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.298)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000014591 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.296)	From Inparanoid:20070104
Dictyostelium discoideum	YP_026175 (score: 0.284) DDB0191323 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000026970 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.295)	From Inparanoid:20070104
Mus musculus	MGI:97454 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.301)	From Inparanoid:20070104
Oryza gramene	YP_026175 (score: 0.306) Q6K6Z6 (score: 1.000; bootstrap: 100%) Q7XDX5 (score: 1.000; bootstrap: 100%) Q947Z0 (score: 0.788) Q9AV23 (score: 0.475) Q8H7R0 (score: 0.454)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000019498 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.299)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000009000 (score: 1.000; bootstrap: 100%) YP_026175 (score: 0.298)	From Inparanoid:20070104
Shigella flexneri	ARSB	From SHIGELLACYC
E. coli O157	ARSB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02040 Arsenical pump membrane protein
EcoCyc	EcoCyc:EG12236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002120	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2148	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011437	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

arsB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to arsB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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