arsB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArsB

Synonyms

arsenite/antimonite transporter[1], B3502[2][1], ArsF[2][1], F172[2][1] , arsF, ECK3487, JW3469, b3502

Product description

ArsB[2][3];

Component of arsenate Ars transporter[2][3]

Arsenite pump, inner membrane; resistance to arsenate, arsenite, and antimonite[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000802

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0046685

response to arsenic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0059

P

Seeded from EcoCyc (v14.0)

complete

GO:0005887

integral to plasma membrane

PMID:20196076[5]

IDA: Inferred from Direct Assay

C

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of arsenate Ars transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized to the periplasm with 11 predicted transmembrane domains

Daley et al. (2005) [6]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MFYPGGIMLL AGAIFVLTIV LVIWQPKGLG IGWSATLGAV LALVTGVVHP GDIPVVWNIV
WNATAAFIAV IIISLLLDES GFFEWAALHV SRWGNGRGRL LFTWIVLLGA AVAALFANDG
AALILTPIVI AMLLALGFSK GTTLAFVMAA GFIADTASLP LIVSNLVNIV SADFFGLGFR
EYASVMVPVD IAAIVATLVM LHLYFRKDIP QNYDMALLKS PAEAIKDPAT FKTGWVVLLL
LLVGFFVLEP LGIPVSAIAA VGALILFVVA KRGHAINTGK VLRGAPWQIV IFSLGMYLVV
YGLRNAGLTE YLSGVLNVLA DNGLWAATLG TGFLTAFLSS IMNNMPTVLV GALSIDGSTA
SGVIKEAMVY ANVIGCDLGP KITPIGSLAT LLWLHVLSQK NMTISWGYYF RTGIIMTLPV
LFVTLAALAL RLSFTL
Length

436

Mol. Wt

46.264 kDa

pI

8.8 (calculated)

Extinction coefficient

80,900 - 81,025 (calc based on 10 Y, 12 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..425

PF02040 Arsenical pump membrane protein

PMID:19920124[7]

<motif_map/>

tmhmm.php?gene=arsB&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=arsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111602

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948011

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011437

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AB93

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12236

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12236

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948011

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002120

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2148

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Hsieh, JM et al. (2010) Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Protein Sci. 19 868-80 PubMed
  6. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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