{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	arpA
Gene Synonym(s)	ECK4009, b4017, JW3977, yjaC, arp^[1]
Product Desc.	regulator of acetyl CoA synthetase^[2]^[3] Ankyrin repeat protein, function unknown^[4]
Product Synonyms(s)	regulator of acetyl CoA synthetase^[1], B4017^[2]^[1], ArpA^[2]^[1], YjaC^[2]^[1], Arp^[2]^[1] , ECK4009, JW3977, yjaC, b4017
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): arpA^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

ArpA was previosly reported to be a regulator of acetyl CoA synthase synthesis based on a personal communication from A. J. Wolfe (1996) cited in SwissProt P23325, however this is now contradicted in Wolfe (2005).^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	arpA
Mnemonic	Ankyrin-like regulatory protein
Synonyms	ECK4009, b4017, JW3977, yjaC, arp^[1]
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	90.92 minutes, 90.92 minutes	MG1655: 4220510..4218324 <gbrowseImage> name=NC_000913:4218324..4220510 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 4108004..4110190 <gbrowseImage> name=NC_012759:4108004..4110190 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4226077..4223891 <gbrowseImage> name=NC_007779:4223891..4226077 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4320206..4318020 <gbrowseImage> name=NC_010473:4318020..4320206 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔarpA (Keio:JW3977)	deletion	deletion	PMID:16738554	Shigen CGSC10860^[5]
ΔarpA784::kan			PMID:16738554	CGSC:100078
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3977	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTACTCGTATTCCTCGTAG Primer 2:CCGCGTACGGTATGGGGAGATGC
6G10	Kohara Phage	Genobase	PMID:3038334
4E5	Kohara Phage	Genobase	PMID:3038334
thiC39::Tn10	Linked marker	CAG18500 = CGSC7472^[5]	est. P1 cotransduction: 41% ^[6] Synonyms:thi-39::Tn10
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 44% ^[6]
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11208	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11208	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001188	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944933	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1193	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013133	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ArpA
Synonyms	regulator of acetyl CoA synthetase^[1], B4017^[2]^[1], ArpA^[2]^[1], YjaC^[2]^[1], Arp^[2]^[1] , ECK4009, JW3977, yjaC, b4017
Product description	regulator of acetyl CoA synthetase^[2]^[3] Ankyrin repeat protein, function unknown^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	bglX	PMID:16606699	Experiment(s):EBI-1147297
Protein	groL	PMID:16606699	Experiment(s):EBI-1147297
Protein	yfbN	PMID:19402753	LCMS(ID Probability):99.6
Protein	alaS	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpmB	PMID:19402753	LCMS(ID Probability):99.6
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MITRIPRSSF SANINNTAQT NEHQTLSELF YKELEDKFSG KELATPLLKS FSENCRQNGR HIFSNKDFVI KFSTSVLQAD KKEITIINKN ENTTLTQTIA PIFEKYLMEI LPQRSDTLDK QELNLKSDRK EKEFPRIKLN GQCYFPGRPQ NRIVCRHIAA QYINDIYQNV DYKPHQDDYS SAEKFLTHFN KKCKNQTLAL VSSRPEGRCV AACGDFGLVM KAYFDKMESN GISVMAAILL VDNHALTVRL RIKNTTEGCT HYVVSVYDPN VTNDKIRIMS ESKENIKHYS LMDFMNVDYS LLKWSNDHVI NQSVAIIPAL PKEQLLMLKG SVDEITPPLS PATMNLLMAI GQNHQLTQLM IQLQKMPELH RTEMLTAYNS INLPGLYLAI NYGNADIVET IFNSLSETGY EGLLSKKNLM HILEAKDKNG FSGLFLAISR KDKNVVTSIL NALPKLAATH HLDNEQVYKF LSAKNRTSSH VLYHVMANGD ADMLKIVLNA LPLLIRTCHL TKEQVLDLLK AKDFYGCPGL YLAMQNGHSD IVKVILEALP SLAQEINISA SDIVDLLTAK SLARDTGLFM AMQRGHMNVI NTIFNALPTL FNTFKFDKKN MKPLLLANNS NEYPGLFSAI QHKQQNVVET VYLALSDHAR LFGFTAEDIM DFWQHKAPQK YSAFELAFEF GHRVIAELIL NTLNKMAESF GFTDNPRYIA EKNYMEALLK KASPHTVR
Length	728
Mol. Wt	82.601 kDa
pI	8.4 (calculated)
Extinction coefficient	48,250 - 49,375 (calc based on 25 Y, 2 W, and 9 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	104..379	PF07906 ShET2 enterotoxin, N-terminal region		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=arpA taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131843	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944933	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013133	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23325	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11208	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11208	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944933	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001188	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1193	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	2.384+/-0.259	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.001829826	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
arpA
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:4220490..4220530 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4017 (EcoliWiki Page)	NCBI GEO profiles for arpA
microarray	GenExpDB:b4017 (EcoliWiki Page)	Summary of data for arpA from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4219997..4220415) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ05; Well:G2^[7]
edit table

Notes

Accessions Related to arpA Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11208	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1193	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4017
EcoGene	EcoGene:EG11208	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001188	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013133	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
edit table

</protect>

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:48403
Pfam (EcoliWiki Page)	PF07906 ShET2 enterotoxin, N-terminal region
EcoCyc	EcoCyc:EG11208	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11208	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001188	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1193	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013133	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

arpA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to arpA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search