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Standard Name	aroH
Gene Synonym(s)	ECK1702, b1704, JW1694^[1], JW1694
Product Desc.	AroH^[2]^[3]; Component of 2-dehydro-3-deoxyphosphoheptonate aldolase^[2]^[3] 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; tryptophan repressible^[4]
Product Synonyms(s)	3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible^[1], B1704^[2]^[1], AroH^[2]^[1] , ECK1702, JW1694, b1704
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aroH^[2], OP00012
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aroH
Mnemonic	Aromatic
Synonyms	ECK1702, b1704, JW1694^[1], JW1694
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	38.5 minutes	MG1655: 1786459..1787505 <gbrowseImage> name=NC_000913:1786459..1787505 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1765703..1766749 <gbrowseImage> name=NC_012967:1765703..1766749 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1678518..1679564 <gbrowseImage> name=NC_012759:1678518..1679564 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1790149..1791195 <gbrowseImage> name=NC_007779:1790149..1791195 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1877030..1878076 <gbrowseImage> name=NC_010473:1877030..1878076 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1786459	Edman degradation	PMID:6167722
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔaroH (Keio:JW1694)	deletion	deletion	PMID:16738554	Shigen CGSC9433^[5]
aroH::Tn5KAN-2 (FB20433)	Insertion at nt 215 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20433	contains pKD46
aroH::Tn5KAN-2 (FB20434)	Insertion at nt 215 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20434	does not contain pKD46
aroH367				CGSC:5310
ΔaroH778::kan			PMID:16738554	CGSC:100070
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1694	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACAGAACTGACGAACTCCG Primer 2:CCGAAGCGGGTATCTACCGCAGA
20F5	Kohara Phage	Genobase	PMID:3038334
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 72% ^[6] Synonyms:zdh-925::Tn10
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 13% ^[6] Synonyms:zdi-276::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000076	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946229	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0078	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005686	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AroH
Synonyms	3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible^[1], B1704^[2]^[1], AroH^[2]^[1] , ECK1702, JW1694, b1704
Product description	AroH^[2]^[3]; Component of 2-dehydro-3-deoxyphosphoheptonate aldolase^[2]^[3] 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; tryptophan repressible^[4]
EC number (for enzymes)	2.5.1.54^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003849	3-deoxy-7-phosphoheptulonate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006219	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003849	3-deoxy-7-phosphoheptulonate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.5.1.54	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008652	cellular amino acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0028	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006218	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006219	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009073	aromatic amino acid family biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0057	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:5335890	IDA: Inferred from Direct Assay		C		complete
	GO:0003849	3-deoxy-7-phosphoheptulonate synthase activity	PMID:5335890	IDA: Inferred from Direct Assay		F		complete
	GO:0009073	aromatic amino acid family biosynthetic process	PMID:5335890	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of 2-dehydro-3-deoxyphosphoheptonate aldolase	could be indirect
Protein	napC		PMID:16606699	Experiment(s):EBI-1140532
Protein	groL		PMID:16606699	Experiment(s):EBI-1140532
Protein	yahI		PMID:16606699	Experiment(s):EBI-1140532
Protein	nadE		PMID:16606699	Experiment(s):EBI-1140532
Protein	matC		PMID:16606699	Experiment(s):EBI-1140532
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED KRLLVIIGPC SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV VGWKGLISDP DLNGSYRVNH GLELARKLLL QVNELGVPTA TEFLDMVTGQ FIADLISWGA IGARTTESQI HREMASALSC PVGFKNGTDG NTRIAVDAIR AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY HADDIAAACD TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTAIAGIMA ESFLREGTQK IVGSQPLTYG QSITDPCLGW EDTERLVEKL ASAVDTRF
Length	348
Mol. Wt	38.735 kDa
pI	6.9 (calculated)
Extinction coefficient	29,910 - 30,785 (calc based on 9 Y, 3 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	24..338	PF00793 DAHP synthetase I family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aroH taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129660	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946229	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005686	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00887	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10080	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946229	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000076	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0078	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1341	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	696	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	765	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aroH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1786439..1786479 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1704 (EcoliWiki Page)	NCBI GEO profiles for aroH
microarray	GenExpDB:b1704 (EcoliWiki Page)	Summary of data for aroH from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1786209..1786474) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:A11^[7]
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Notes

Accessions Related to aroH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10080	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0078	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1704
EcoGene	EcoGene:EG10080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000076	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005686	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000026229 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000028397 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YBR249C (score: 1.000; bootstrap: 100%) YDR035W (score: 0.176)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC24H60c (score: 1.000; bootstrap: 100%) SPAP8A37c (score: 0.133)	From Inparanoid:20070104
Shigella flexneri	AROH	From SHIGELLACYC
E. coli O157	AROH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00793 DAHP synthetase I family
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
EcoCyc	EcoCyc:EG10080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10080	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000076	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0078	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005686	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

aroH:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aroH Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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