argG:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

argG

Gene Synonym(s)

ECK3161, b3172, JW3140, Arg6[1], Arg6, argE

Product Desc.

ArgG[2][3];

Component of argininosuccinate synthase[3]

Argininosuccinate synthase[4]

Product Synonyms(s)

argininosuccinate synthetase[1], B3172[2][1], ArgG[2][1] , Arg6, argE, ECK3161, JW3140, b3172

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): argG[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argG

Mnemonic

Arginine

Synonyms

ECK3161, b3172, JW3140, Arg6[1], Arg6, argE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.48 minutes 

MG1655: 3316659..3318002
<gbrowseImage> name=NC_000913:3316659..3318002 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3253871..3255214
<gbrowseImage> name=NC_012967:3253871..3255214 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3203807..3205150
<gbrowseImage> name=NC_012759:3203807..3205150 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3318492..3319835
<gbrowseImage> name=NC_007779:3318492..3319835 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3414404..3415747
<gbrowseImage> name=NC_010473:3414404..3415747 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3316662

Edman degradation

PMID:2123815
PMID:9298646


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔargG (Keio:JW3140)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen

CGSC8306[5]

argG::Tn5KAN-I-SceI (FB21055)

Insertion at nt 895 in Minus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB21055

contains pKD46

argG::Tn5KAN-I-SceI (FB21056)

Insertion at nt 895 in Minus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB21056

does not contain pKD46

argG6

Auxotrophies

CGSC:4887

argG34

Auxotrophies

CGSC:5148

argG35

Auxotrophies

CGSC:5150

argG53

Auxotrophies

CGSC:5168

argG66

Auxotrophies

CGSC:5182

argG75

Auxotrophies

CGSC:5191

argG77

Auxotrophies

CGSC:5193

argG78

Auxotrophies

CGSC:5194

argG83

Auxotrophies

CGSC:5199

ΔargG781::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:99800


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3140

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACGACGATTCTCAAGCATCT

Primer 2:CCtTGGCCTTTGTTTTCCAGATT

E4G11S

Kohara Phage

Genobase

PMID:3038334

18H7

Kohara Phage

Genobase

PMID:3038334

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[5]

est. P1 cotransduction: % [6]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[5]

est. P1 cotransduction: 60% [6]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10068

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10068

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000064

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947590

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0066

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010428

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArgG

Synonyms

argininosuccinate synthetase[1], B3172[2][1], ArgG[2][1] , Arg6, argE, ECK3161, JW3140, b3172

Product description

ArgG[2][3];

Component of argininosuccinate synthase[3]

Argininosuccinate synthase[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004055

argininosuccinate synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00581

F

Seeded from EcoCyc (v14.0)

complete

GO:0004055

argininosuccinate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001518

F

Seeded from EcoCyc (v14.0)

complete

GO:0004055

argininosuccinate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018223

F

Seeded from EcoCyc (v14.0)

complete

GO:0004055

argininosuccinate synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.4.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00581

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001518

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018223

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00581

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00581

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001518

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018223

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0055

P

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of argininosuccinate synthase

could be indirect

Protein

uvrA

PMID:16606699

Experiment(s):EBI-1145035

Protein

matC

PMID:16606699

Experiment(s):EBI-1145035

Protein

kefA

PMID:16606699

Experiment(s):EBI-1145035

Protein

glyA

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTTILKHLPV GQRIGIAFSG GLDTSAALLW MRQKGAVPYA YTANLGQPDE EDYDAIPRRA
MEYGAENARL IDCRKQLVAE GIAAIQCGAF HNTTGGLTYF NTTPLGRAVT GTMLVAAMKE
DGVNIWGDGS TYKGNDIERF YRYGLLTNAE LQIYKPWLDT DFIDELGGRH EMSEFMIACG
FDYKMSVEKA YSTDSNMLGA THEAKDLEYL NSSVKIVNPI MGVKFWDESV KIPAEEVTVR
FEQGHPVALN GKTFSDDVEM MLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
HIAYERLLTG IHNEDTIEQY HAHGRQLGRL LYQGRWFDSQ ALMLRDSLQR WVASQITGEV
TLELRRGNDY SILNTVSENL TYKPERLTME KGDSVFSPDD RIGQLTMRNL DITDTREKLF
GYAKTGLLSS SAASGVPQVE NLENKGQ
Length

447

Mol. Wt

49.899 kDa

pI

5.2 (calculated)

Extinction coefficient

61,310 - 61,685 (calc based on 19 Y, 6 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6E4

Domain

15..418

PF00764 Arginosuccinate synthase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=argG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131063

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947590

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010428

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6E4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10068

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10068

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947590

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000064

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0066

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

5.37E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

38.07+/-0.552

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.044303797

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 EMG2

380

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M74

PMID: 9298646

Protein

E. coli K-12 MG1655

1296

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

8231

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

497

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

argG

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3316639..3316679 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3172 (EcoliWiki Page)

NCBI GEO profiles for argG

microarray

GenExpDB:b3172 (EcoliWiki Page)

Summary of data for argG from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3315845..3316322) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ01; Well:B11[7]

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Notes

Accessions Related to argG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10068

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0066

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3172

EcoGene

EcoGene:EG10068

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000064

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010428

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000018209 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000027264 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT4G24830 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000027649 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000029548 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000013423 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040912-178 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040912-180 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0026565 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA12079-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000006379 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000253004 (score: 1.000; bootstrap: 100%)
  • ENSP00000249376 (score: 0.119)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000025233 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000002024 (score: 0.929)
  • ENSMMUP00000008353 (score: 0.766)
  • ENSMMUP00000012187 (score: 0.736)
  • ENSMMUP00000002218 (score: 0.592)
  • ENSMMUP00000005189 (score: 0.554)
  • ENSMMUP00000011101 (score: 0.406)
  • ENSMMUP00000010808 (score: 0.361)
  • ENSMMUP00000008412 (score: 0.215)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000015146 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:88090 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q53QJ9 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000036742 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000012075 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOL058W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC4285c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000137718 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00017163001 (score: 1.000; bootstrap: 100%)
  • GSTENP00008399001 (score: 0.268)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000046083 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ARGG

From SHIGELLACYC

E. coli O157

ARGG

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00764 Arginosuccinate synthase

Superfamily (EcoliWiki Page)

SUPERFAMILY:52402

Panther (EcoliWiki Page)

PTHR11587:SF0

Superfamily (EcoliWiki Page)

SUPERFAMILY:69864

EcoCyc

EcoCyc:EG10068

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10068

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000064

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0066

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010428

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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