argC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

argC

Gene Synonym(s)

ECK3949, b3958, JW3930, Arg2[1], Arg2, argH

Product Desc.

N- N-acetyl-gamma-glutamyl-phosphate reductase[2]

Product Synonyms(s)

N-acetyl-gamma-glutamylphosphate reductase, NAD(P)-binding[1], B3958[3][1], ArgC[3][1] , Arg2, argH, ECK3949, JW3930, b3958

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): argCBH[3], OP00005

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argC

Mnemonic

Arginine

Synonyms

ECK3949, b3958, JW3930, Arg2[1], Arg2, argH

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

89.51 minutes 

MG1655: 4153024..4154028
<gbrowseImage> name=NC_000913:4153024..4154028 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4134862..4135866
<gbrowseImage> name=NC_012967:4134862..4135866 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4042693..4043697
<gbrowseImage> name=NC_012759:4042693..4043697 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3481680..3480676
<gbrowseImage> name=NC_007779:3480676..3481680 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4252721..4253725
<gbrowseImage> name=NC_010473:4252721..4253725 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔargC (Keio:JW3930)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen

CGSC10839[4]

ΔargC::kan

deletion

Biolog:respiration

unable to respire D-Sorbitol

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire DL-a-Glycerol

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire D-Galactoniate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire D-Galacturonate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire D-Glucuronate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire DL-Lactate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire Glucuronamide

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

ΔargC::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

argC32

Auxotrophies

CGSC:5730

argC24

Auxotrophies

CGSC:5985

ΔargC745::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:100040


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3930

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTTGAATACGCTGATTGTGGG

Primer 2:CCAATAAGAGACTGCGTTTCCGC

4G11

Kohara Phage

Genobase

PMID:3038334

8H10

Kohara Phage

Genobase

PMID:3038334

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[4]

est. P1 cotransduction: 98% [5]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[4]

est. P1 cotransduction: 17% [5]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10065

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10065

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000061

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948455

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0063

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012964

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArgC

Synonyms

N-acetyl-gamma-glutamylphosphate reductase, NAD(P)-binding[1], B3958[3][1], ArgC[3][1] , Arg2, argH, ECK3949, JW3930, b3958

Product description

N- N-acetyl-gamma-glutamyl-phosphate reductase[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003942

N-acetyl-gamma-glutamyl-phosphate reductase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00150

F

Seeded from EcoCyc (v14.0)

complete

GO:0003942

N-acetyl-gamma-glutamyl-phosphate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000706

F

Seeded from EcoCyc (v14.0)

complete

GO:0003942

N-acetyl-gamma-glutamyl-phosphate reductase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.2.1.38

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00150

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012280

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00150

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000706

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0055

P

Seeded from EcoCyc (v14.0)

complete

GO:0046983

protein dimerization activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012280

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLNTLIVGAS GYAGAELVTY VNRHPHMNIT ALTVSAQSND AGKLISDLHP QLKGIVDLPL
QPMSDISEFS PGVDVVFLAT AHEVSHDLAP QFLEAGCVVF DLSGAFRVND ATFYEKYYGF
THQYPELLEQ AAYGLAEWCG NKLKEANLIA VPGCYPTAAQ LALKPLIDAD LLDLNQWPVI
NATSGVSGAG RKAAISNSFC EVSLQPYGVF THRHQPEIAT HLGADVIFTP HLGNFPRGIL
ETITCRLKSG VTQAQVAQVL QQAYAHKPLV RLYDKGVPAL KNVVGLPFCD IGFAVQGEHL
IIVATEDNLL KGAAAQAVQC ANIRFGYAET QSLI
Length

334

Mol. Wt

35.951 kDa

pI

5.9 (calculated)

Extinction coefficient

28,880 - 29,755 (calc based on 12 Y, 2 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..146

PF01118 Semialdehyde dehydrogenase, NAD binding domain

PMID:19920124

Domain

163..311

PF02774 Semialdehyde dehydrogenase, dimerisation domain

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=argC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131796

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948455

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012964

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P11446

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10065

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10065

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948455

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000061

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0063

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

57

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1076

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

39a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

argCBH

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4153004..4153044 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3958 (EcoliWiki Page)

NCBI GEO profiles for argC

microarray

GenExpDB:b3958 (EcoliWiki Page)

Summary of data for argC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4152351..4152619) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ01; Well:C6[6]

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Notes

Accessions Related to argC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10065

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0063

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3958

EcoGene

EcoGene:EG10065

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000061

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012964

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Apis mellifera

  • ENSAPMP00000020080 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT2G19940 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231462 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6AV34 (score: 1.000; bootstrap: 100%)
  • Q8LNT4 (score: 0.847)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YER069W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC4G99c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ARGC

From SHIGELLACYC

E. coli O157

ARGC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Superfamily (EcoliWiki Page)

SUPERFAMILY:55347

Pfam (EcoliWiki Page)

PF01118 Semialdehyde dehydrogenase, NAD binding domain

Pfam (EcoliWiki Page)

PF02774 Semialdehyde dehydrogenase, dimerisation domain

EcoCyc

EcoCyc:EG10065

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10065

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000061

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0063

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012964

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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