aphA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aphA

Mnemonic

Acid phosphatase

Synonyms

ECK4047, b4055, JW4015, yjbP, hobH[1], hobH, napA

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.98 minutes 

MG1655: 4267437..4268150
<gbrowseImage> name=NC_000913:4267437..4268150 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4248346..4249059
<gbrowseImage> name=NC_012967:4248346..4249059 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4206282..4206995
<gbrowseImage> name=NC_012759:4206282..4206995 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4273004..4273717
<gbrowseImage> name=NC_007779:4273004..4273717 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4367133..4367846
<gbrowseImage> name=NC_010473:4367133..4367846 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4267512

Edman degradation

PMID:9011040[2]
PMID:9298646[3]
PMID:9987119[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔaphA (Keio:JW4015)

deletion

deletion

PMID:16738554[5]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4015

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCCGCAAGATCACACAGGCAAT

Primer 2:CCGTATTCTGAATTGACGATCAC

1E4

Kohara Phage

Genobase

PMID:3038334[7]

12D1

Kohara Phage

Genobase

PMID:3038334[7]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[8]

est. P1 cotransduction: 36% [9]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[8]

est. P1 cotransduction: 20% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11934

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11934

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001862

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948562

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1878

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013283

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Thaller, MC et al. (1997) Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product. FEMS Microbiol. Lett. 146 191-8 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Reshetnyak, E et al. (1999) Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase. Mol. Microbiol. 31 167-75 PubMed
  5. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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