PMID:9987119
| Citation |
Reshetnyak, E, d'Alençon, E, Kern, R, Taghbalout, A, Guillaud, P and Kohiyama, M (1999) Hemi-methylated oriC DNA binding activity found in non-specific acid phosphatase. Mol. Microbiol. 31:167-75 |
|---|---|
| Abstract |
The lacZ-hobH fusion clone, containing an Escherichia coli DNA segment located at 92 min on the chromosomal map, was screened as a producer of E. coli oriC hemi-methylated binding activity. We have purified the protein encoded by this locus to near homogeneity. The protein corresponds to the monomeric form of a non-specific acid phosphatase (NAP) whose gene has been designated aphA. oriC DNA footprinting experiments showed protection of hemi-methylated probe by partially purified NAP, but not by purified preparations. Yet, gel retardation experiments with an oriC oligonucleotide demonstrated DNA binding activity of purified NAP in the presence of Mg2+. This experiment also showed an increased affinity of the protein for the hemi-methylated probe compared with the fully or unmethylated form. Indirect immunofluorescene microscopy revealed the existence of discrete NAP foci at mid-cell in cells with two nucleoids, but at cell poles in those with one nucleoid. |
| Links | |
| Keywords |
Acid Phosphatase/metabolism; Cell Membrane/metabolism; DNA Footprinting; DNA Methylation; DNA-Binding Proteins/metabolism; Escherichia coli/enzymology; Replication Origin; Transformation, Bacterial |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
