PMID:9011040
| Citation |
Thaller, MC, Schippa, S, Bonci, A, Cresti, S and Rossolini, GM (1997) Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product. FEMS Microbiol. Lett. 146:191-8 |
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| Abstract |
An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties were analyzed. The enzyme is a 100-kDa homotetrameric protein which apparently requires a metal co-factor for activity. Similarly to other bacterial class B acid phosphatases, it is able to dephosphorylate several organic phosphomonoesters as well as to catalyze the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds. |
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| Keywords |
Acid Phosphatase/genetics; Acid Phosphatase/isolation & purification; Acid Phosphatase/metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial/physiology; Gene Expression Regulation, Enzymologic/physiology; Genes, Bacterial/genetics; Molecular Sequence Data; Recombinant Proteins |
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