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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	amyA
Gene Synonym(s)	ECK1926, b1927, JW1912, yedC^[1], yedC
Product Desc.	ALPHA-AMYL-CYTO^[2]^[3] alpha-Amylase, cytoplasmic^[4]
Product Synonyms(s)	cytoplasmic alpha-amylase^[1], B1927^[2]^[1], YedC^[2]^[1], AmyA^[2]^[1] , ECK1926, JW1912, yedC, b1927
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): amyA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	amyA
Mnemonic	Amylase
Synonyms	ECK1926, b1927, JW1912, yedC^[1], yedC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	43.2 minutes	MG1655: 2004180..2005667 <gbrowseImage> name=NC_000913:2004180..2005667 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1896663..1898150 <gbrowseImage> name=NC_012759:1896663..1898150 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2008293..2009780 <gbrowseImage> name=NC_007779:2008293..2009780 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2095188..2096675 <gbrowseImage> name=NC_010473:2095188..2096675 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔamyA (Keio:JW1912)	deletion	deletion	PMID:16738554	Shigen CGSC9590^[5]
amyA::Tn5KAN-2 (FB20533)	Insertion at nt 654 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20533	contains pKD46
amyA::Tn5KAN-2 (FB20534)	Insertion at nt 654 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20534	does not contain pKD46
ΔamyA773::kan			PMID:16738554	CGSC:100008
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1912	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGTAATCCCACGCTGTTACA Primer 2:CCAATCACCTCTTCGATAACCCA
4B9	Kohara Phage	Genobase	PMID:3038334
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[5]	est. P1 cotransduction: 62% ^[6]
zed-3069::Tn10	Linked marker	CAG18451 = CGSC7395^[5]	est. P1 cotransduction: 27% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001354	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946434	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1360	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006414	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AmyA
Synonyms	cytoplasmic alpha-amylase^[1], B1927^[2]^[1], YedC^[2]^[1], AmyA^[2]^[1] , ECK1926, JW1912, yedC, b1927
Product description	ALPHA-AMYL-CYTO^[2]^[3] alpha-Amylase, cytoplasmic^[4]
EC number (for enzymes)	3.2.1.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016052	carbohydrate catabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000272	polysaccharide catabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006047	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006589	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013781	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013776	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004556	alpha-amylase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.2.1.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005509	calcium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013776	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005509	calcium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0106	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006047	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006589	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013776	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013781	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0119	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016798	hydrolase activity, acting on glycosyl bonds	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	F	Seeded from EcoCyc (v14.0)	complete
	GO:0043169	cation binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006047	F	Seeded from EcoCyc (v14.0)	complete
	GO:0043169	cation binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006589	F	Seeded from EcoCyc (v14.0)	complete
	GO:0043169	cation binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013781	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	pflA	PMID:16606699	Experiment(s):EBI-1141384
Protein	guaA	PMID:16606699	Experiment(s):EBI-1141384
Protein	phoB	PMID:16606699	Experiment(s):EBI-1141384
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG GYSVGYDSYD LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD VVVNHKMGAD EKEAIRVQRV NADDRTQIDE EIIECEGWTR YTFPARAGQY SQFIWDFKCF SGIDHIENPD EDGIFKIVND YTGEGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL ARQRFAHGVQ TLFFDHPNCI AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG ENYGNKTWRD FLGNRQERVV TDENGEATFF CNGGSVSVWV IEEVI
Length	495
Mol. Wt	56.639 kDa
pI	4.4 (calculated)
Extinction coefficient	109,780 - 110,655 (calc based on 22 Y, 14 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	21..376	PF00128 Alpha amylase, catalytic domain		PMID:19920124
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<motif_map/>

Structure
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Structures

<beststructure> gene=amyA taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129874	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946434	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006414	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P26612	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11387	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946434	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001354	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1360	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	16	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	398	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
amyA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2004160..2004200 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1927 (EcoliWiki Page)	NCBI GEO profiles for amyA
microarray	GenExpDB:b1927 (EcoliWiki Page)	Summary of data for amyA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2004025..2004229) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:E7^[7]
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Notes

Accessions Related to amyA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11387	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1360	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1927
EcoGene	EcoGene:EG11387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001354	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006414	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G69830 (score: 1.000; bootstrap: 100%) AT1G76130 (score: 0.196) AT4G25000 (score: 0.153)	From Inparanoid:20070104
Oryza gramene	P27932 (score: 1.000; bootstrap: 100%) P08117 (score: 1.000; bootstrap: 100%) Q67U05 (score: 0.996) Q6ZDD6 (score: 0.758) P27934 (score: 0.758) Q67U03 (score: 0.756) P27937 (score: 0.756) Q41770 (score: 0.749) Q67U02 (score: 0.743) Q6ZDD5 (score: 0.739) P27933 (score: 0.739) P27939 (score: 0.734) Q03651 (score: 0.641) P04063 (score: 0.634) P04750 (score: 0.621) O81699 (score: 0.617) Q6Z317 (score: 0.616) P00693 (score: 0.615) P27935 (score: 0.615) Q40017 (score: 0.614) Q40015 (score: 0.614) P17654 (score: 0.613) Q5Z7T8 (score: 0.613) Q6Z319 (score: 0.609) Q7Y1C3 (score: 0.609) O04965 (score: 0.607) O81700 (score: 0.606) Q40016 (score: 0.602) P27941 (score: 0.596) Q42504 (score: 0.588) Q5QLB1 (score: 0.587) O04964 (score: 0.583) Q40018 (score: 0.550) P04747 (score: 0.542) Q5QLB2 (score: 0.392) Q7XSC8 (score: 0.347) Q6V7G2 (score: 0.279) Q6V7E3 (score: 0.279) Q4U1I8 (score: 0.190) Q4U1I7 (score: 0.189) P04748 (score: 0.179) P04749 (score: 0.150) P27940 (score: 0.101)	From Inparanoid:20070104
Shigella flexneri	AMYA	From SHIGELLACYC
E. coli O157	AMYA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00128 Alpha amylase, catalytic domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:51011
Superfamily (EcoliWiki Page)	SUPERFAMILY:51445
Panther (EcoliWiki Page)	PTHR10357:SF9
EcoCyc	EcoCyc:EG11387	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11387	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001354	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1360	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006414	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

amyA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to amyA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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