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Product(s) |

Expression|

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Standard Name	ampG
Gene Synonym(s)	ECK0427, b0433, JW0423^[1], JW0423
Product Desc.	AmpG muropeptide MFS transporter^[2]^[3] Muropeptide permease, imports GlcNAc-anhMurNAc-peptides; required for cloned Citrobacter freundii AmpR-regulated beta-lactamase induction and murein recycling^[4]
Product Synonyms(s)	muropeptide transporter^[1], B0433^[2]^[1], AmpG^[2]^[1] , ECK0427, JW0423, b0433
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ampG^[2]
Regulation/Activity
Quick Links
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Notes

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Imported muropeptides must contain the disaccharide GlcNAc-anhMurNAc, which is also efficiently transported without attached peptides. Mutants accumulate GlcNAc-anhMurNAc-tripeptide. Transport inhibited by CCCP. 14 TM helices are [predicted, but fusion studies indicate that predicted hydrophobic helices 5, 6, 11, and 12 are in cytoplasmic loops; AmpG probably has 10 TM helices with N-in, C-in topology. AmpG has been studied using cloned Citrobacter freundii ampR and ampC genes, which are normally on the chromosome of C. freundii. This C. freundii ampC is not the same as the E. coli weak constitutive chromosomal ampC beta-lactamase; E. coli has no ampR gene. Major Facilitator Superfamily (MFS).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ampG
Mnemonic	Ampicillin
Synonyms	ECK0427, b0433, JW0423^[1], JW0423
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	9.73 minutes	MG1655: 452769..451294 <gbrowseImage> name=NC_000913:451294..452769 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 424250..422775 <gbrowseImage> name=NC_012967:422775..424250 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 354053..355528 <gbrowseImage> name=NC_012759:354053..355528 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 452769..451294 <gbrowseImage> name=NC_007779:451294..452769 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 392100..390625 <gbrowseImage> name=NC_010473:390625..392100 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ampGG268D		G268D	(in non-inducible mutant SN0301- 3)			Strain variation; seeded from UniProt:P0AE16
ampGG373D		G373D	(in non-inducible mutant SN0301- 5)			Strain variation; seeded from UniProt:P0AE16
ampGG151D		G151D	(in non-inducible mutant SN0301- 1)			Strain variation; seeded from UniProt:P0AE16
ampG(del) (Keio:JW0423)	deletion	deletion		PMID:16738554	Shigen CGSC8587^[5]
ampG::Tn5KAN-2 (FB20173)	Insertion at nt 490 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20173	does not contain pKD46
ampG790(del)::kan				PMID:16738554	CGSC:100004
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0423	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCCAGTCAATATTTACGTAT Primer 2:CCCGTCAGATGCGTTTTTCGTAG
2H5	Kohara Phage	Genobase	PMID:3038334
3B6	Kohara Phage	Genobase	PMID:3038334
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 49% ^[6]
zaj-3054::Tn10	Linked marker	CAG12017 = CGSC7339^[5]	est. P1 cotransduction: 76% ^[6] Synonyms:zba-3054::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12183	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12183	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002076	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946438	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2100	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001501	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AmpG
Synonyms	muropeptide transporter^[1], B0433^[2]^[1], AmpG^[2]^[1] , ECK0427, JW0423, b0433
Product description	AmpG muropeptide MFS transporter^[2]^[3] Muropeptide permease, imports GlcNAc-anhMurNAc-peptides; required for cloned Citrobacter freundii AmpR-regulated beta-lactamase induction and murein recycling^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011701	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 14 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSSQYLRIFQ QPRSAILLIL GFASGLPLAL TSGTLQAWMT VENIDLKTIG FFSLVGQAYV FKFLWSPLMD RYTPPFFGRR RGWLLATQIL LLVAIAAMGF LEPGTQLRWM AALAVVIAFC SASQDIVFDA WKTDVLPAEE RGAGAAISVL GYRLGMLVSG GLALWLADKW LGWQGMYWLM AALLIPCIIA TLLAPEPTDT IPVPKTLEQA VVAPLRDFFG RNNAWLILLL IVLYKLGDAF AMSLTTTFLI RGVGFDAGEV GVVNKTLGLL ATIVGALYGG ILMQRLSLFR ALLIFGILQG ASNAGYWLLS ITDKHLYSMG AAVFFENLCG GMGTSAFVAL LMTLCNKSFS ATQFALLSAL SAVGRVYVGP VAGWFVEAHG WSTFYLFSVA AAVPGLILLL VCRQTLEYTR VNDNFISRTA YPAGYAFAMW TLAAGVSLLA VWLLLLTMDA LDLTHFSFLP ALLEVGVLVA LSGVVLGGLL DYLALRKTHL T
Length	491
Mol. Wt	53.247 kDa
pI	8.4 (calculated)
Extinction coefficient	104,850 - 105,475 (calc based on 15 Y, 15 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	17..369	PF07690 Major Facilitator Superfamily		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ampG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128418	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946438	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001501	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AE16	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12183	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12183	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946438	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002076	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2100	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	259	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	55	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	75	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ampG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:452749..452789 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0433 (EcoliWiki Page)	NCBI GEO profiles for ampG
microarray	GenExpDB:b0433 (EcoliWiki Page)	Summary of data for ampG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ampG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12183	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2100	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0433
EcoGene	EcoGene:EG12183	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002076	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001501	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000010312 (score: 1.000; bootstrap: 90%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000002415 (score: 1.000; bootstrap: 76%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000015181 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040426-1241 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-2174 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000301327 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000020448 (score: 1.000; bootstrap: 71%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000013750 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1916822 (score: 1.000; bootstrap: 75%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000035427 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000021208 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000183112 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000031159 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Shigella flexneri	AMPG	From SHIGELLACYC
E. coli O157	AMPG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR12778:SF2
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
Pfam (EcoliWiki Page)	PF07690 Major Facilitator Superfamily
EcoCyc	EcoCyc:EG12183	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12183	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002076	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2100	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001501	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

ampG:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ampG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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