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Expression|

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Standard Name	ampC
Gene Synonym(s)	ECK4146, b4150, JW4111, ampA^[1], ampA
Product Desc.	β-lactamase; penicillin resistance^[2]^[3] Intrinsic weak beta-lactamase activity; penicillin resistance; affects morphology; penicillin-binding protein^[4]
Product Synonyms(s)	beta-lactamase/D-alanine carboxypeptidase^[1], B4150^[2]^[1], AmpA^[2]^[1], AmpC^[2]^[1] , ampA, ECK4146, JW4111, b4150
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ampC^[2]
Regulation/Activity
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Notes

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False positive lipoprotein prediction. Verified SPI substrate.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ampC
Mnemonic	Ampicillin
Synonyms	ECK4146, b4150, JW4111, ampA^[1], ampA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.31 minutes, 94.31 minutes	MG1655: 4376967..4375834 <gbrowseImage> name=NC_000913:4375834..4376967 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4357655..4356522 <gbrowseImage> name=NC_012967:4356522..4357655 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4314569..4315702 <gbrowseImage> name=NC_012759:4314569..4315702 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4383624..4382491 <gbrowseImage> name=NC_007779:4382491..4383624 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4477329..4476196 <gbrowseImage> name=NC_010473:4476196..4477329 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4375837	Edman degradation	PMID:6795623
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ampCS80D,E,G		S80D,E,G		Loss of activity			seeded from UniProt:P00811
ampCK83Q,T		K83Q,T		Lowers activity more than 1000- fold and increases protein stability			seeded from UniProt:P00811
ampCK331A		K331A		Lowers activity more than 1000- fold			seeded from UniProt:P00811
ampCN168D,H		N168D,H		Lowers activity more than 1000- fold			seeded from UniProt:P00811
ampCY166E		Y166E		Lowers activity more than 1000- fold			seeded from UniProt:P00811
ampC(del) (Keio:JW4111)	deletion	deletion			PMID:16738554	Shigen CGSC10960^[5]
ampC12						CGSC:10039
ampC11						CGSC:11804
ampC3						CGSC:11809
ampC777(del)::kan					PMID:16738554	CGSC:99998
ampC			Resistant to	ampicillin resistance	PMID:4945191
ampC	Gene duplication in the ampC gene region		Resistant to	Ampicillin-hyperresistant mutants of Ecoli K12 with multiple gene duplications in the ampC gene region overproduced at least six proteins during anaerobic growth. All but two of the proteins were also overproduced during aerobic growth.	PMID:391567
ampA1			Resistant to	Resistant to Penicillins	PMID:391567		Referenced At: PMID:334718 & PMID:4904235
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4111	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTCAAAACGACGCTCTGCGC Primer 2:CCtTGTAGAGCGTTAAGAATCTG
1D4	Kohara Phage	Genobase	PMID:3038334
3H6	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 50% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 10% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10040	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10040	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000036	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948669	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0038	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013592	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AmpC
Synonyms	beta-lactamase/D-alanine carboxypeptidase^[1], B4150^[2]^[1], AmpA^[2]^[1], AmpC^[2]^[1] , ampA, ECK4146, JW4111, b4150
Product description	β-lactamase; penicillin resistance^[2]^[3] Intrinsic weak beta-lactamase activity; penicillin resistance; affects morphology; penicillin-binding protein^[4]
EC number (for enzymes)	3.5.2.6^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008800	beta-lactamase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001586	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008800	beta-lactamase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.5.2.6	F	Seeded from EcoCyc (v14.0)	complete
	GO:0017001	antibiotic catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001586	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008800	beta-lactamase activity	PMID:9324260	IDA: Inferred from Direct Assay		F		complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001586	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1147567
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm		PMID:6795623	EchoLocation:ampC
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFKTTLCALL ITASCSTFAA PQQINDIVHR TITPLIEQQK IPGMAVAVIY QGKPYYFTWG YADIAKKQPV TQQTLFELGS VSKTFTGVLG GDAIARGEIK LSDPTTKYWP ELTAKQWNGI TLLHLATYTA GGLPLQVPDE VKSSSDLLRF YQNWQPAWAP GTQRLYANSS IGLFGALAVK PSGLSFEQAM QTRVFQPLKL NHTWINVPPA EEKNYAWGYR EGKAVHVSPG ALDAEAYGVK STIEDMARWV QSNLKPLDIN EKTLQQGIQL AQSRYWQTGD MYQGLGWEML DWPVNPDSII NGSDNKIALA ARPVKAITPP TPAVRASWVH KTGATGGFGS YVAFIPEKEL GIVMLANKNY PNPARVDAAW QILNALQ
Length	377
Mol. Wt	41.556 kDa
pI	9.1 (calculated)
Extinction coefficient	93,850 - 94,100 (calc based on 15 Y, 13 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-19	UniProt Manual:Signal Peptides	UniProt:P00811
Modification Site	80	phosphorylation site at S80	probability greater than 75%	PMID:17938405
Domain	25..377	PF00144 Beta-lactamase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ampC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131975	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948669	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013592	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00811	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10040	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10040	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000036	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0038	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.47E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	587	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	132	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	177	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ampC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4376947..4376987 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4150 (EcoliWiki Page)	NCBI GEO profiles for ampC
microarray	GenExpDB:b4150 (EcoliWiki Page)	Summary of data for ampC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ampC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10040	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0038	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4150
EcoGene	EcoGene:EG10040	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000036	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013592	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	AMPC	From SHIGELLACYC
E. coli O157	AMPC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00144 Beta-lactamase
Panther (EcoliWiki Page)	PTHR22935:SF9
Superfamily (EcoliWiki Page)	SUPERFAMILY:56601
EcoCyc	EcoCyc:EG10040	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10040	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000036	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0038	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013592	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ampC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ampC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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