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Expression|

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Standard Name	amn
Gene Synonym(s)	ECK1977, b1982, JW1963^[1], JW1963
Product Desc.	Amn^[2]^[3]; Component of AMP nucleosidase^[2]^[3] AMP nucleosidase^[4]
Product Synonyms(s)	AMP nucleosidase^[1], B1982^[2]^[1], Amn^[2]^[1] , ECK1977, JW1963, b1982
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): amn^[2]
Regulation/Activity
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Notes

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PhoB regulon (Baek, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	amn
Mnemonic	AMP nucleosidase
Synonyms	ECK1977, b1982, JW1963^[1], JW1963
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
BW2952		NC_012759: 1945568..1947022 <gbrowseImage> name=NC_012759:1945568..1947022 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
MG1655	44.25 minutes	MG1655: 2053085..2054539 <gbrowseImage> name=NC_000913:2053085..2054539 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1993060..1994514 <gbrowseImage> name=NC_012967:1993060..1994514 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 2057198..2058652 <gbrowseImage> name=NC_007779:2057198..2058652 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2144093..2145547 <gbrowseImage> name=NC_010473:2144093..2145547 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2053085	Edman degradation	PMID:2690948
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δamn (Keio:JW1963)	deletion	deletion	PMID:16738554	Shigen CGSC9628^[5]
amn::Tn5KAN-2 (FB20556)	Insertion at nt 239 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20556	contains pKD46
Δamn-756::kan			PMID:16738554	CGSC:99996
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1963	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAATAAGGGCTCCGGTCT Primer 2:CCTCGGAACGGCGGCTCATTAAA
7D4	Kohara Phage	Genobase	PMID:3038334
9B2	Kohara Phage	Genobase	PMID:3038334
zed-3069::Tn10	Linked marker	CAG18451 = CGSC7395^[5]	est. P1 cotransduction: 56% ^[6]
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 31% ^[6] Synonyms:zee-3129::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000035	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946508	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0037	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Amn
Synonyms	AMP nucleosidase^[1], B1982^[2]^[1], Amn^[2]^[1] , ECK1977, JW1963, b1982
Product description	Amn^[2]^[3]; Component of AMP nucleosidase^[2]^[3] AMP nucleosidase^[4]
EC number (for enzymes)	3.2.2.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000845	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008714	AMP nucleosidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.2.2.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009116	nucleoside metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000845	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of AMP nucleosidase	could be indirect
Protein	livJ		PMID:16606699	Experiment(s):EBI-1141551
Protein	secA		PMID:16606699	Experiment(s):EBI-1141551
Protein	priA		PMID:16606699	Experiment(s):EBI-1141551
Protein	hrpB		PMID:16606699	Experiment(s):EBI-1141551
Protein	groL		PMID:16606699	Experiment(s):EBI-1141551
Protein	lsrA		PMID:16606699	Experiment(s):EBI-1141551
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNNKGSGLTP AQALDKLDAL YEQSVVALRN AIGNYITSGE LPDENARKQG LFVYPSLTVT WDGSTTNPPK TRAFGRFTHA GSYTTTITRP TLFRSYLNEQ LTLLYQDYGA HISVQPSQHE IPYPYVIDGS ELTLDRSMSA GLTRYFPTTE LAQIGDETAD GIYHPTEFSP LSHFDARRVD FSLARLRHYT GTPVEHFQPF VLFTNYTRYV DEFVRWGCSQ ILDPDSPYIA LSCAGGNWIT AETEAPEEAI SDLAWKKHQM PAWHLITADG QGITLVNIGV GPSNAKTICD HLAVLRPDVW LMIGHCGGLR ESQAIGDYVL AHAYLRDDHV LDAVLPPDIP IPSIAEVQRA LYDATKLVSG RPGEEVKQRL RTGTVVTTDD RNWELRYSAS ALRFNLSRAV AIDMESATIA AQGYRFRVPY GTLLCVSDKP LHGEIKLPGQ ANRFYEGAIS EHLQIGIRAI DLLRAEGDRL HSRKLRTFNE PPFR
Length	484
Mol. Wt	53.995 kDa
pI	6.3 (calculated)
Extinction coefficient	71,280 - 71,905 (calc based on 22 Y, 7 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	229..452	PF01048 Phosphorylase superfamily	PMID:19920124
Domain	11..172	PF10423 Bacterial AMP nucleoside phosphorylase N-terminus	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=amn taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129926	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946508	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006579	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AE12	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10039	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946508	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000035	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0037	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.47E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	521	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1104	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	541	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
amn
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2053065..2053105 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1982 (EcoliWiki Page)	NCBI GEO profiles for amn
microarray	GenExpDB:b1982 (EcoliWiki Page)	Summary of data for amn from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2052906..2053134) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ05; Well:A9^[7]
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Notes

Accessions Related to amn Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10039	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0037	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1982
EcoGene	EcoGene:EG10039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000035	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000014197 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AMN	From SHIGELLACYC
E. coli O157	AMN	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR21234:SF11
Superfamily (EcoliWiki Page)	SUPERFAMILY:53167
Pfam (EcoliWiki Page)	PF01048 Phosphorylase superfamily
Pfam (EcoliWiki Page)	PF10423 Bacterial AMP nucleoside phosphorylase N-terminus
EcoCyc	EcoCyc:EG10039	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10039	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000035	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0037	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

amn:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to amn Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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