alaS:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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<protect>

Standard Name

alaS

Gene Synonym(s)

ECK2692, b2697, JW2667, act, ala-act, lovB, ala-a[1]

Product Desc.

alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3];

Component of alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3]

Alanine--tRNA ligase[4]

Product Synonyms(s)

alanyl-tRNA synthetase[1], Act[2][1], B2697[2][1], LovB[2][1], AlaS[2][1], ala-act[2][1] , act, ala-act, ECK2692, JW2667, lovB, b2697

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): alaS[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

alaS

Mnemonic

Alanine

Synonyms

ECK2692, b2697, JW2667, act, ala-act, lovB, ala-a[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

60.72 minutes 

MG1655: 2820033..2817403
<gbrowseImage> name=NC_000913:2817403..2820033 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2716870..2714240
<gbrowseImage> name=NC_012967:2714240..2716870 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2703215..2705845
<gbrowseImage> name=NC_012759:2703215..2705845 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2820667..2818037
<gbrowseImage> name=NC_007779:2818037..2820667 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2912575..2909945
<gbrowseImage> name=NC_010473:2909945..2912575 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2817406

Edman degradation

PMID:7005898


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔalaS (Keio:JW2667)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen CGSC10104[5]

Strain contains duplication detected by PCR PMID:18652654

alaSE184Q

E184Q

Auxotrophies

No inactivation

seeded from UniProt:P00957

alaSD188N

D188N

Auxotrophies

No inactivation

seeded from UniProt:P00957

alaSH189Q

H189Q

Auxotrophies

Inactivates the enzyme

seeded from UniProt:P00957

alaSD191N

D191N

Auxotrophies

No inactivation

seeded from UniProt:P00957

alaSH192Q

H192Q

Auxotrophies

Inactivates the enzyme

seeded from UniProt:P00957

alaSC179S

C179S

Auxotrophies

Inactivates the enzyme

seeded from UniProt:P00957

alaSC182S

C182S

Auxotrophies

No inactivation

seeded from UniProt:P00957

alaS5(ts)

Growth Phenotype

temperature sensitive

CGSC:9418

alaS4(ts)

Growth Phenotype

temperature sensitive

CGSC:9420

alaS6(ts)

Growth Phenotype

temperature sensitive

CGSC:9435

alaS3(ts)

missense mutation

Growth Phenotype

temperature sensitive - no immediate cessation of protein synthesis but residual protein increases for long time, inclusion of 1% NaCl in medium permits colony formation above non-permissive temperature

PMID:796671

CGSC:27060

single-site mutation in structural gene of alanyl-tRNA synthetase

ΔalaS772::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:99960

alaS21

Resistant to

Resistant to B lactam Mecillinam

PMID:1563353

Strain: GC3702

See table 2

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Notes

The Keio collection[6] lists a deletion of alaS. The insertion in this strain is a duplication of the alaS region.[7]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2667

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCAAGAGCACCGCTGAGAT

Primer 2:CCTTGCAATTTCGCGCTGACCCA

23B7

Kohara Phage

Genobase

PMID:3038334

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[5]

est. P1 cotransduction: % [8]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: 76% [8]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10034

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10034

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000031

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947175

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0033

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008869

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AlaS

Synonyms

alanyl-tRNA synthetase[1], Act[2][1], B2697[2][1], LovB[2][1], AlaS[2][1], ala-act[2][1] , act, ala-act, ECK2692, JW2667, lovB, b2697

Product description

alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3];

Component of alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3]

Alanine--tRNA ligase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000049

tRNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0820

F

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002318

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018163

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003156

F

Seeded from EcoCyc (v14.0)

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

GO:0004813

alanine-tRNA ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00036

F

Seeded from EcoCyc (v14.0)

complete

GO:0004813

alanine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

F

Seeded from EcoCyc (v14.0)

complete

GO:0004813

alanine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

F

Seeded from EcoCyc (v14.0)

complete

GO:0004813

alanine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

F

Seeded from EcoCyc (v14.0)

complete

GO:0004813

alanine-tRNA ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.1.1.7

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00036

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012947

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00036

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002318

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012947

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012947

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006419

alanyl-tRNA aminoacylation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00036

P

Seeded from EcoCyc (v14.0)

complete

GO:0006419

alanyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018162

P

Seeded from EcoCyc (v14.0)

complete

GO:0006419

alanyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018164

P

Seeded from EcoCyc (v14.0)

complete

GO:0006419

alanyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018165

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of alanyl-tRNA synthetase

could be indirect

Protein

ydfR

PMID:15690043

Experiment(s):EBI-883462

Protein

dnaK

PMID:15690043

Experiment(s):EBI-883462, EBI-883577

Protein

rpsB

PMID:15690043

Experiment(s):EBI-883462

Protein

tufA

PMID:15690043

Experiment(s):EBI-883462

Protein

rspA

PMID:16606699

Experiment(s):EBI-1143684

Protein

yfaA

PMID:16606699

Experiment(s):EBI-1143684

Protein

bcsB

PMID:16606699

Experiment(s):EBI-1143684

Protein

gltX

PMID:19402753

MALDI(Z-score):26.003989

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSKSTAEIRQ AFLDFFHSKG HQVVASSSLV PHNDPTLLFT NAGMNQFKDV FLGLDKRNYS
RATTSQRCVR AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKHDAIQF AWELLTSEKW
FALPKERLWV TVYESDDEAY EIWEKEVGIP RERIIRIGDN KGAPYASDNF WQMGDTGPCG
PCTEIFYDHG DHIWGGPPGS PEEDGDRYIE IWNIVFMQFN RQADGTMEPL PKPSVDTGMG
LERIAAVLQH VNSNYDIDLF RTLIQAVAKV TGATDLSNKS LRVIADHIRS CAFLIADGVM
PSNENRGYVL RRIIRRAVRH GNMLGAKETF FYKLVGPLID VMGSAGEDLK RQQAQVEQVL
KTEEEQFART LERGLALLDE ELAKLSGDTL DGETAFRLYD TYGFPVDLTA DVCRERNIKV
DEAGFEAAME EQRRRAREAS GFGADYNAMI RVDSASEFKG YDHLELNGKV TALFVDGKAV
DAINAGQEAV VVLDQTPFYA ESGGQVGDKG ELKGANFSFA VEDTQKYGQA IGHIGKLAAG
SLKVGDAVQA DVDEARRARI RLNHSATHLM HAALRQVLGT HVSQKGSLVN DKVLRFDFSH
NEAMKPEEIR AVEDLVNTQI RRNLPIETNI MDLEAAKAKG AMALFGEKYD ERVRVLSMGD
FSTELCGGTH ASRTGDIGLF RIISESGTAA GVRRIEAVTG EGAIATVHAD SDRLSEVAHL
LKGDSNNLAD KVRSVLERTR QLEKELQQLK EQAAAQESAN LSSKAIDVNG VKLLVSELSG
VEPKMLRTMV DDLKNQLGST IIVLATVVEG KVSLIAGVSK DVTDRVKAGE LIGMVAQQVG
GKGGGRPDMA QAGGTDAAAL PAALASVKGW VSAKLQ
Length

876

Mol. Wt

96.032 kDa

pI

5.7 (calculated)

Extinction coefficient

70,820 - 71,570 (calc based on 18 Y, 8 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

799..869

PF02272 DHHA1 domain

PMID:19920124

Domain

7..556

PF01411 tRNA synthetases class II (A)

PMID:19920124

Domain

653..696

PF07973 Threonyl and Alanyl tRNA synthetase second additional domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=alaS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130604

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947175

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008869

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00957

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10034

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10034

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947175

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000031

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0033

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.83E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

86.147+/-0.477

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.243536122

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

8493

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1795

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

5773

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

alaS

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

alaS regulates it's own transcription [9]

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2820013..2820053 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2697 (EcoliWiki Page)

NCBI GEO profiles for alaS

microarray

GenExpDB:b2697 (EcoliWiki Page)

Summary of data for alaS from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2819936..2820163) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:B11[10]

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Notes

Accessions Related to alaS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10034

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0033

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2697

EcoGene

EcoGene:EG10034

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000031

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008869

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013174 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000003987 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G22800 (score: 1.000; bootstrap: 89%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000026393 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000018232 (score: 0.129)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00033089 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00000197 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000029841 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000002926 (score: 0.166)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000015190 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030131-3663 (score: 1.000; bootstrap: 100%)
  • ZDB-GENE-050913-159 (score: 0.320)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0214894 (score: 1.000; bootstrap: 49%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0027094 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA12250-PA (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000003043 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000016505 (score: 0.233)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000261772 (score: 1.000; bootstrap: 100%)
  • ENSP00000244571 (score: 0.177)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000018898 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000018141 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000023615 (score: 0.163)

From Inparanoid:20070104

Mus musculus

  • MGI:2384560 (score: 1.000; bootstrap: 100%)
  • MGI:2681839 (score: 0.179)

From Inparanoid:20070104

Oryza gramene

  • Q94GG8 (score: 1.000; bootstrap: 74%)
  • Q7XGA3 (score: 1.000; bootstrap: 77%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000014170 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000031143 (score: 0.176)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000025051 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000037568 (score: 0.174)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR335C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC23C119 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000160311 (score: 1.000; bootstrap: 74%)
  • NEWSINFRUP00000144283 (score: 0.092)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00006546001 (score: 1.000; bootstrap: 100%)
  • GSTENP00008928001 (score: 0.052)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000016680 (score: 1.000; bootstrap: 55%)

From Inparanoid:20070104

Shigella flexneri

ALAS

From SHIGELLACYC

E. coli O157

ALAS

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02272 DHHA1 domain

Panther (EcoliWiki Page)

PTHR11777:SF6

Superfamily (EcoliWiki Page)

SUPERFAMILY:55186

Superfamily (EcoliWiki Page)

SUPERFAMILY:55681

Superfamily (EcoliWiki Page)

SUPERFAMILY:101353

Pfam (EcoliWiki Page)

PF01411 tRNA synthetases class II (A)

Pfam (EcoliWiki Page)

PF07973 Threonyl and Alanyl tRNA synthetase second additional domain

EcoCyc

EcoCyc:EG10034

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10034

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000031

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0033

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008869

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. ↑ Putney, SD & Schimmel, P (1981) An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature 291 632-5 PubMed
  10. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

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