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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	acpS
Gene Synonym(s)	ECK2561, b2563, JW2547, dpj, G220^[1]^[2]
Product Desc.	AcpS^[2]^[3]; Component of [[:Category:Complex:holo-[acyl-carrier-protein] synthase\|holo-[acyl-carrier-protein] synthase]]^[2]^[3] ACP-CoA phosphopantetheinyltransferase; Holo-ACP synthase^[4]
Product Synonyms(s)	holo-[acyl-carrier-protein] synthase 1^[1], B2563^[2]^[1], Dpj^[2]^[1], AcpS^[2]^[1] , dpj, ECK2561, JW2547, b2563
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pdxJ-acpS^[2], rnc-era-recO-pdxJ-acpS^[2]
Regulation/Activity
Quick Links
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Notes

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acpS(dpj) was demonstrated to be an essential gene (Takiff, 1992), consistent with the results of Baba (2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	acpS
Mnemonic	Acyl carrier protein
Synonyms	ECK2561, b2563, JW2547, dpj, G220^[1]^[2]
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	58.16 minutes, 58.16 minutes	MG1655: 2699020..2698640 <gbrowseImage> name=NC_000913:2698640..2699020 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2622490..2622110 <gbrowseImage> name=NC_012967:2622110..2622490 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2584445..2584825 <gbrowseImage> name=NC_012759:2584445..2584825 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2699654..2699274 <gbrowseImage> name=NC_007779:2699274..2699654 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2698643	Edman degradation	PMID:7559576
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Comments
acpSG5D		G5D		5-fold reduction in catalytic activity		seeded from UniProt:P24224
acpS1	G4D substitution: G to A transition in the fifth codon (underlined), GCAATATTTAGATX_x	G4D	Auxotrophies	G4D mutation causes a 5 fold reduction in catalytic efficiency, Fig. 7,8,9.	PMID:10625633	Mutant K-12 strain used was e. coli MP4.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2547	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAATATTAGGTTTAGGCAC Primer 2:CCACTTTCAATAATTACCGTGGC
7G4	Kohara Phage	Genobase	PMID:3038334
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 24% ^[6] Synonyms:zff-208::Tn10
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 69% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10247	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10247	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000241	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947037	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0243	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008433	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AcpS
Synonyms	holo-[acyl-carrier-protein] synthase 1^[1], B2563^[2]^[1], Dpj^[2]^[1], AcpS^[2]^[1] , dpj, ECK2561, JW2547, b2563
Product description	AcpS^[2]^[3]; Component of [[:Category:Complex:holo-[acyl-carrier-protein] synthase\|holo-[acyl-carrier-protein] synthase]]^[2]^[3] ACP-CoA phosphopantetheinyltransferase; Holo-ACP synthase^[4]
EC number (for enzymes)	2.7.8.7^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002582	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004568	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00101	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00101	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002582	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004568	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0275	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008610	lipid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0444	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00101	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002582	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004568	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.7.8.7	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009059	macromolecule biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	PMID:7559576^[7]	TAS: Traceable Author Statement		F		complete
	GO:0006633	fatty acid biosynthetic process	PMID:7559576^[7]	NAS: Non-traceable Author Statement		P		complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	PMID:7559576^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0005625	soluble fraction	PMID:7559576^[7]	IDA: Inferred from Direct Assay		C		complete
	GO:0042803	protein homodimerization activity	PMID:7559576^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0018070	peptidyl-serine phosphopantetheinylation	PMID:14294075^[8]	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of [[:Category:Complex:holo-[acyl-carrier-protein] synthase\|holo-[acyl-carrier-protein] synthase]]	could be indirect
Protein	ybiB		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	rplD		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	groL		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	rplF		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	rplB		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	oxyR		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	rpsG		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	yfeK		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	yphE		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	rpsC		PMID:16606699^[9]	Experiment(s):EBI-1143271
Protein	tufB		PMID:19402753^[10]	MALDI(Z-score):18.215873
Protein	murC		PMID:19402753^[10]	MALDI(Z-score):24.922879
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL AKRFAVKEAA AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE KLGVANMHVT LADERHYACA TVIIES
Length	126
Mol. Wt	14.052 kDa
pI	9.6 (calculated)
Extinction coefficient	17,990 - 18,115 (calc based on 1 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P24224
Domain	6..73	PF01648 4'-phosphopantetheinyl transferase superfamily		PMID:19920124^[11]
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<motif_map/>

Structure
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Structures

<beststructure> gene=acpS taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130488	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947037	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008433	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P24224	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10247	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10247	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947037	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000241	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0243	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	11.815+/-0.083	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.064473684	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	372	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	142	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	99^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pdxJ-acpS
rnc-era-recO-pdxJ-acpS
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2699000..2699040 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2563 (EcoliWiki Page)	NCBI GEO profiles for acpS
microarray	GenExpDB:b2563 (EcoliWiki Page)	Summary of data for acpS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to acpS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10247	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0243	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2563
EcoGene	EcoGene:EG10247	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000241	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008433	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Dictyostelium discoideum	DDB0217726 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACPS	From SHIGELLACYC
E. coli O157	ACPS	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01648 4'-phosphopantetheinyl transferase superfamily
Superfamily (EcoliWiki Page)	SUPERFAMILY:56214
EcoCyc	EcoCyc:EG10247	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10247	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000241	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0243	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008433	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 Lambalot, RH & Walsh, CT (1995) Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. J. Biol. Chem. 270 24658-61 PubMed
↑ MAJERUS, PW et al. (1965) ACYL CARRIER PROTEIN. IV. THE IDENTIFICATION OF 4'-PHOSPHOPANTETHEINE AS THE PROSTHETIC GROUP OF THE ACYL CARRIER PROTEIN. Proc. Natl. Acad. Sci. U.S.A. 53 410-7 PubMed
↑ ^9.00 ^9.01 ^9.02 ^9.03 ^9.04 ^9.05 ^9.06 ^9.07 ^9.08 ^9.09 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ ^10.0 ^10.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

acpS:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acpS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

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