acpS:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
acpS |
---|---|
Gene Synonym(s) | |
Product Desc. |
Component of [[:Category:Complex:holo-[acyl-carrier-protein] synthase|holo-[acyl-carrier-protein] synthase]][2][3] ACP-CoA phosphopantetheinyltransferase; Holo-ACP synthase[4] |
Product Synonyms(s) |
holo-[acyl-carrier-protein] synthase 1[1], B2563[2][1], Dpj[2][1], AcpS[2][1] , dpj, ECK2561, JW2547, b2563 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression |
transcription unit(s): pdxJ-acpS[2], rnc-era-recO-pdxJ-acpS[2] |
Regulation/Activity | |
Quick Links | |
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Notes
acpS(dpj) was demonstrated to be an essential gene (Takiff, 1992), consistent with the results of Baba (2006).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
acpS |
---|---|
Mnemonic |
Acyl carrier protein |
Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
58.16 minutes, 58.16 minutes |
MG1655: 2699020..2698640 |
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NC_012967: 2622490..2622110 |
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NC_012759: 2584445..2584825 |
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W3110 |
|
W3110: 2699654..2699274 |
||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
2698643 |
Edman degradation |
PMID:7559576 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
acpSG5D |
G5D |
5-fold reduction in catalytic activity |
seeded from UniProt:P24224 | ||||
acpS1 |
G4D substitution: G to A transition in the fifth codon (underlined), GCAATATTTAGATXx |
G4D |
Auxotrophies |
G4D mutation causes a 5 fold reduction in catalytic efficiency, Fig. 7,8,9. |
PMID:10625633 |
Mutant K-12 strain used was e. coli MP4. | |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW2547 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGCAATATTAGGTTTAGGCAC Primer 2:CCACTTTCAATAATTACCGTGGC | |
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 24% [6] | ||
Linked marker |
est. P1 cotransduction: 69% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000241 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0243 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008433 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
AcpS |
---|---|
Synonyms |
holo-[acyl-carrier-protein] synthase 1[1], B2563[2][1], Dpj[2][1], AcpS[2][1] , dpj, ECK2561, JW2547, b2563 |
Product description |
Component of [[:Category:Complex:holo-[acyl-carrier-protein] synthase|holo-[acyl-carrier-protein] synthase]][2][3] ACP-CoA phosphopantetheinyltransferase; Holo-ACP synthase[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000287 |
magnesium ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002582 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000287 |
magnesium ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004568 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000287 |
magnesium ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008278 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000287 |
magnesium ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0460 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00101 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006633 |
fatty acid biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00101 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006633 |
fatty acid biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002582 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006633 |
fatty acid biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004568 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006633 |
fatty acid biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0275 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008610 |
lipid biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0444 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00101 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002582 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004568 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008278 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.7.8.7 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009059 |
macromolecule biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008278 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0046872 |
metal ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0479 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016740 |
transferase activity |
PMID:7559576 |
TAS: Traceable Author Statement |
F |
complete | |||
GO:0006633 |
fatty acid biosynthetic process |
PMID:7559576 |
NAS: Non-traceable Author Statement |
P |
complete | |||
GO:0008897 |
holo-(acyl-carrier-protein) synthase activity |
PMID:7559576 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0005625 |
soluble fraction |
PMID:7559576 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0042803 |
protein homodimerization activity |
PMID:7559576 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0018070 |
peptidyl-serine phosphopantetheinylation |
PMID:14294075 |
IDA: Inferred from Direct Assay |
P |
complete | |||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of [[:Category:Complex:holo-[acyl-carrier-protein] synthase|holo-[acyl-carrier-protein] synthase]] |
could be indirect |
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Protein |
ybiB |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
rplD |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
groL |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
rplF |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
rplB |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
oxyR |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
rpsG |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
yfeK |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
yphE |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
rpsC |
PMID:16606699 |
Experiment(s):EBI-1143271 | |
Protein |
tufB |
PMID:19402753 |
MALDI(Z-score):18.215873 | |
Protein |
murC |
PMID:19402753 |
MALDI(Z-score):24.922879 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL AKRFAVKEAA AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE KLGVANMHVT LADERHYACA TVIIES |
Length |
126 |
Mol. Wt |
14.052 kDa |
pI |
9.6 (calculated) |
Extinction coefficient |
17,990 - 18,115 (calc based on 1 Y, 3 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0008433 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000241 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0243 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
11.815+/-0.083 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.064473684 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
372 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
142 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
99a |
molecules/cell/generation |
|
Ribosome Profiling |
Low confidence in the sequencing data set. |
PMID: 24766808 |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2699000..2699040
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for acpS | |
microarray |
Summary of data for acpS from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to acpS Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0243 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000241 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008433 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Shigella flexneri |
ACPS |
From SHIGELLACYC |
E. coli O157 |
ACPS |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10247 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000241 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0243 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008433 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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