acpP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

acpP

Gene Synonym(s)

ECK1080, b1094, JW1080[1], JW1080

Product Desc.

lipoyl-ACP[2][3]

Product Synonyms(s)

acyl carrier protein (ACP)[1], B1094[2][1] , ECK1080, JW1080, b1094

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): fabG-acpP[2], fabDG-acpP[2], acpP-fabF[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

acpP

Mnemonic

Acyl carrier protein

Synonyms

ECK1080, b1094, JW1080[1], JW1080

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

BW2952

NC_012759: 1054763..1054999
<gbrowseImage> name=NC_012759:1054763..1054999 source=BW2952 preset=GeneLocation </gbrowseImage>

MG1655

24.8 minutes 

MG1655: 1150838..1151074
<gbrowseImage> name=NC_000913:1150838..1151074 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1166184..1166420
<gbrowseImage> name=NC_012967:1166184..1166420 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1153192..1153428
<gbrowseImage> name=NC_007779:1153192..1153428 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1206283..1206519
<gbrowseImage> name=NC_010473:1206283..1206519 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1150841

Edman degradation

PMID:2062368
PMID:3549687
PMID:4882207
PMID:9119048


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

acpPS37A,T

S37A,T

Loss of phosphopantetheinylation, and inhibition of cell growth

seeded from UniProt:P0A6A8

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1080

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCACTATCGAAGAACGCGT

Primer 2:CCgGCCTGGTGGCCGTTGATGTA

14C1

Kohara Phage

Genobase

PMID:3038334

E9G1

Kohara Phage

Genobase

PMID:3038334

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[4]

est. P1 cotransduction: % [5]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[4]

est. P1 cotransduction: 86% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50003

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50003

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002562

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944805

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4297

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003703

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AcpP

Synonyms

acyl carrier protein (ACP)[1], B1094[2][1] , ECK1080, JW1080, b1094

Product description

lipoyl-ACP[2][3]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000036

acyl carrier activity

PMID:15459190

IDA: Inferred from Direct Assay

F

complete

GO:0006633

fatty acid biosynthetic process

PMID:16352845

IMP: Inferred from Mutant Phenotype

P

complete

GO:0008610

lipid biosynthetic process

PMID:16352845

IMP: Inferred from Mutant Phenotype

P

complete

GO:0031177

phosphopantetheine binding

PMID:16352845

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005737

cytoplasm

PMID:15459190

IDA: Inferred from Direct Assay

C

complete

GO:0042493

response to drug

PMID:15459190

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0042493

response to drug

PMID:15459190

IMP: Inferred from Mutant Phenotype

P

N-pentylpantothenamide and N-heptylpantothenamide

complete

GO:0000035

acyl binding

PMID:15459190

IDA: Inferred from Direct Assay

F

complete

GO:0009245

lipid A biosynthetic process

PMID:16352845

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

mukB

PMID:15690043

Experiment(s):EBI-878935, EBI-894594

Protein

plsB

PMID:15690043

Experiment(s):EBI-878935

Protein

ribF

PMID:15690043

Experiment(s):EBI-878935

Protein

rpsG

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

secA

PMID:15690043

Experiment(s):EBI-878935

Protein

spoT

PMID:15690043

Experiment(s):EBI-878935, EBI-886668

Protein

ybgC

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

ybgJ

PMID:15690043

Experiment(s):EBI-878935

Protein

yiiD

PMID:15690043

Experiment(s):EBI-878935, EBI-882138, EBI-887888

Protein

aas

PMID:15690043

Experiment(s):EBI-878935

Protein

aceE

PMID:15690043

Experiment(s):EBI-878935

Protein

aceF

PMID:15690043

Experiment(s):EBI-878935

Protein

acpS

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

aidB

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

yccX

PMID:15690043

Experiment(s):EBI-878935

Protein

insO2

PMID:15690043

Experiment(s):EBI-878935

Protein

fabB

PMID:15690043

Experiment(s):EBI-878935

Protein

fabF

PMID:15690043

Experiment(s):EBI-878935, EBI-882138, EBI-893240

Protein

fabG

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

fabH

PMID:15690043

Experiment(s):EBI-878935

Protein

fabZ

PMID:15690043

Experiment(s):EBI-878935

Protein

glmU

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

lpdA

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

lpxD

PMID:15690043

Experiment(s):EBI-878935, EBI-882138

Protein

malT

PMID:15690043

Experiment(s):EBI-878935

Protein

accA

PMID:15690043

Experiment(s):EBI-882138

Protein

hupB

PMID:15690043

Experiment(s):EBI-882138

Protein

rplO

PMID:15690043

Experiment(s):EBI-882138

Protein

rplV

PMID:15690043

Experiment(s):EBI-882138

Protein

rpmB

PMID:15690043

Experiment(s):EBI-882138

Protein

rpsF

PMID:15690043

Experiment(s):EBI-882138

Protein

rpsH

PMID:15690043

Experiment(s):EBI-882138

Protein

rpsM

PMID:15690043

Experiment(s):EBI-882138

Protein

yhbY

PMID:15690043

Experiment(s):EBI-882138

Protein

rpsM

PMID:19402753

LCMS(ID Probability):99.6

Protein

accA

PMID:19402753

LCMS(ID Probability):99.6

Protein

hupB

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.6

Protein

lpxD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):37.890659

Protein

rplO

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsH

PMID:19402753

LCMS(ID Probability):99.6

Protein

plsB

PMID:19402753

MALDI(Z-score):28.115910

Protein

aidB

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):28.483346

Protein

yiiD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):39.085879

Protein

ybgC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):25.238576

Protein

fabB

PMID:19402753

MALDI(Z-score):47.576368

Protein

fabF

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):38.711597

Protein

fabG

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

ribF

PMID:19402753

MALDI(Z-score):36.845456

Protein

acpS

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):21.026827

Protein

aas

PMID:19402753

MALDI(Z-score):28.197629

Protein

glmU

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):39.768280

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSTIEERVKK IIGEQLGVKQ EEVTNNASFV EDLGADSLDT VELVMALEEE FDTEIPDEEA
EKITTVQAAI DYINGHQA
Length

78

Mol. Wt

8.638 kDa

pI

3.8 (calculated)

Extinction coefficient

1,490 (calc based on 1 Y, 0 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6A8

Domain

6..73

PF00550 Phosphopantetheine attachment site

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=acpP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129057

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944805

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003703

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6A8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG50003

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50003

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944805

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002562

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4297

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.02E+04

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

357865

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

85961

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

214887

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fabG-acpP

fabDG-acpP

acpP-fabF

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1150818..1150858 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1094 (EcoliWiki Page)

NCBI GEO profiles for acpP

microarray

GenExpDB:b1094 (EcoliWiki Page)

Summary of data for acpP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1150546..1150884) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ07; Well:F2[6]

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Notes

Accessions Related to acpP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50003

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4297

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1094

EcoGene

EcoGene:EG50003

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002562

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003703

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000026688 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000019113 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G65290 (score: 1.000; bootstrap: 85%)
  • AT2G44620 (score: 0.289)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000008382 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00034091 (score: 1.000; bootstrap: 100%)
  • WBGene00030157 (score: 0.189)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00013237 (score: 1.000; bootstrap: 100%)
  • WBGene00017508 (score: 0.284)
  • WBGene00018151 (score: 0.191)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000026059 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000025950 (score: 0.588)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040801-1 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040801-1 (score: 1.000; bootstrap: 100%)
  • ZDB-GENE-030131-4437 (score: 0.395)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0184099 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0011361 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21583-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000009787 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000007516 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000027028 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000020437 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1917566 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6AUP3 (score: 1.000; bootstrap: 87%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000024471 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000043027 (score: 0.142)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YKL192C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC4H39 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000143607 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00021872001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ACPP

From SHIGELLACYC

E. coli O157

ACPP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:47336

Pfam (EcoliWiki Page)

PF00550 Phosphopantetheine attachment site

EcoCyc

EcoCyc:EG50003

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50003

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002562

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4297

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003703

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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