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Gene |

Product(s) |

Expression|

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Standard Name	ackA
Gene Synonym(s)	ECK2290, b2296, JW2293^[1], JW2293
Product Desc.	Acetate kinase, produces acetyl phosphate; mutants are fluoroacetate resistant; creBC regulon; binds Zn(II)^[2]
Product Synonyms(s)	acetate kinase A and propionate kinase 2^[1], B2296^[3]^[1], AckA^[3]^[1], propionate kinase 2^[3]^[1], acetate kinase A^[3]^[1] , ECK2290, JW2293, b2296
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ackA-pta^[3], ackA^[3]
Regulation/Activity
Quick Links
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Notes

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N-terminus is blocked (Fox, 86).^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ackA
Mnemonic	Acetate kinase
Synonyms	ECK2290, b2296, JW2293^[1], JW2293
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	51.98 minutes	MG1655: 2411492..2412694 <gbrowseImage> name=NC_000913:2411492..2412694 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2357062..2358264 <gbrowseImage> name=NC_012967:2357062..2358264 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2297297..2298499 <gbrowseImage> name=NC_012759:2297297..2298499 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2418916..2420118 <gbrowseImage> name=NC_007779:2418916..2420118 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2503257..2504459 <gbrowseImage> name=NC_010473:2503257..2504459 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔackA (Keio:JW2293)	deletion	deletion	PMID:16738554	Shigen CGSC9843^[4]
ackA202				CGSC:25451
ackA51::kan				CGSC:82727
ΔackA778::kan			PMID:16738554	CGSC:99892
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2293	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGAGTAAGTTAGTACTGGT Primer 2:CCGGCAGTCAGGCGGCTCGCGTC
9C2	Kohara Phage	Genobase	PMID:3038334
E9C9	Kohara Phage	Genobase	PMID:3038334
zfb-223::Tn10	Linked marker	CAG18484 = CGSC7406^[4]	est. P1 cotransduction: 44% ^[5] Synonyms:zej-223::Tn10
zfd-1::Tn10	Linked marker	CAG18467 = CGSC7408^[4]	est. P1 cotransduction: % ^[5] Synonyms:zfb-1::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10027	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10027	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000024	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946775	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0026	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AckA
Synonyms	acetate kinase A and propionate kinase 2^[1], B2296^[3]^[1], AckA^[3]^[1], propionate kinase 2^[3]^[1], acetate kinase A^[3]^[1] , ECK2290, JW2293, b2296
Product description	Acetate kinase, produces acetyl phosphate; mutants are fluoroacetate resistant; creBC regulon; binds Zn(II)^[2]
EC number (for enzymes)	2.7.2.-^[1] 2.7.2.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006082	organic acid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004372	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008776	acetate kinase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00020	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008776	acetate kinase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.7.2.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0016310	phosphorylation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00020	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016310	phosphorylation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000890	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019542	propionate biosynthetic process	PMID:9484901	IGI: Inferred from Genetic Interaction	EcoliWiki:tdcD	P		complete
	GO:0019413	acetate biosynthetic process	PMID:9484901	IGI: Inferred from Genetic Interaction	EcoliWiki:tdcD	P		complete
	GO:0008776	acetate kinase activity	PMID:2536666	IDA: Inferred from Direct Assay		F		complete
	GO:0008270	zinc ion binding	PMID:11985624	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	hemX	PMID:16606699	Experiment(s):EBI-1142466
Protein	ybgA	PMID:16606699	Experiment(s):EBI-1142466
Protein	def	PMID:16606699	Experiment(s):EBI-1142466
Protein	hyfE	PMID:16606699	Experiment(s):EBI-1142466
Protein	fur	PMID:16606699	Experiment(s):EBI-1142466
Protein	kdsD	PMID:16606699	Experiment(s):EBI-1142466
Protein	greB	PMID:16606699	Experiment(s):EBI-1142466
Protein	glyA	PMID:16606699	Experiment(s):EBI-1142466
Protein	moaC	PMID:16606699	Experiment(s):EBI-1142466
Protein	rpmB	PMID:16606699	Experiment(s):EBI-1142466
Protein	ahpC	PMID:16606699	Experiment(s):EBI-1142466
Protein	greA	PMID:16606699	Experiment(s):EBI-1142466
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSSKLVLVLN CGSSSLKFAI IDAVNGEEYL SGLAECFHLP EARIKWKMDG NKQEAALGAG AAHSEALNFI VNTILAQKPE LSAQLTAIGH RIVHGGEKYT SSVVIDESVI QGIKDAASFA PLHNPAHLIG IEEALKSFPQ LKDKNVAVFD TAFHQTMPEE SYLYALPYNL YKEHGIRRYG AHGTSHFYVT QEAAKMLNKP VEELNIITCH LGNGGSVSAI RNGKCVDTSM GLTPLEGLVM GTRSGDIDPA IIFHLHDTLG MSVDAINKLL TKESGLLGLT EVTSDCRYVE DNYATKEDAK RAMDVYCHRL AKYIGAYTAL MDGRLDAVVF TGGIGENAAM VRELSLGKLG VLGFEVDHER NLAARFGKSG FINKEGTRPA VVIPTNEELV IAQDASRLTA
Length	400
Mol. Wt	43.29 kDa
pI	6.2 (calculated)
Extinction coefficient	24,870 - 25,620 (calc based on 13 Y, 1 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..393	PF00871 Acetokinase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ackA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130231	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946775	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007579	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6A3	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10027	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10027	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946775	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000024	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0026	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.09E+04	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	201.673+/-0.875	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.111064892	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	35607	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1197	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	15918	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ackA-pta
ackA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2411472..2411512 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2296 (EcoliWiki Page)	NCBI GEO profiles for ackA
microarray	GenExpDB:b2296 (EcoliWiki Page)	Summary of data for ackA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2411075..2411541) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ03; Well:F5^[6]
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Notes

Accessions Related to ackA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10027	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0026	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2296
EcoGene	EcoGene:EG10027	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000024	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000002466 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000023519 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACKA	From SHIGELLACYC
E. coli O157	ACKA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00871 Acetokinase family
Panther (EcoliWiki Page)	PTHR21060:SF14
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
EcoCyc	EcoCyc:EG10027	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10027	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000024	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0026	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007579	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 ^3.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

ackA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ackA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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