PMID:2536666
| Citation |
Matsuyama, A, Yamamoto, H and Nakano, E (1989) Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene. J. Bacteriol. 171:577-80 |
|---|---|
| Abstract |
The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome. |
| Links | |
| Keywords |
Acetate Kinase/biosynthesis; Acetate Kinase/genetics; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular; Codon/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Bacterial; Molecular Sequence Data; Phosphotransferases/genetics; Plasmids |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
