PMID:2536666
Citation |
Matsuyama, A, Yamamoto, H and Nakano, E (1989) Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene. J. Bacteriol. 171:577-80 |
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Abstract |
The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome. |
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Keywords |
Acetate Kinase/biosynthesis; Acetate Kinase/genetics; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular; Codon/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Bacterial; Molecular Sequence Data; Phosphotransferases/genetics; Plasmids |
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