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Gene |

Product(s) |

Expression|

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Standard Name	aceF
Gene Synonym(s)	ECK0114, b0115, JW0111^[1], JW0111
Product Desc.	AceF-lipoate^[2]^[3], AceF-S-acetyldihydrolipoate^[2]^[3] Pyruvate dehydrogenase, dihydrolipoamide acetyltransferase E2; acetate requirement^[4]
Product Synonyms(s)	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2^[1], B0115^[2]^[1] , ECK0114, JW0111, b0115
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aceEF^[2], pdhR-aceEF-lpdA^[2]
Regulation/Activity
Quick Links
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Notes

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Holoenzyme is AceE(24)+AceF(24)+Lpd(12). HT_Cmplx3_Cyt: AceF+Lpd.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aceF
Mnemonic	Acetate
Synonyms	ECK0114, b0115, JW0111^[1], JW0111
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
BW2952		NC_012759: 125694..127586 <gbrowseImage> name=NC_012759:125694..127586 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
MG1655	2.71 minutes	MG1655: 125695..127587 <gbrowseImage> name=NC_000913:125695..127587 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 128499..130391 <gbrowseImage> name=NC_012967:128499..130391 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 125695..127587 <gbrowseImage> name=NC_007779:125695..127587 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 99799..101691 <gbrowseImage> name=NC_010473:99799..101691 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	125698	Edman degradation	PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
aceF(del) (Keio:JW0111)	deletion	deletion	Auxotrophies		PMID:16738554	Shigen CGSC8393^[5]
aceFH603C		H603C	Auxotrophies	Abolishes catalytic activity			seeded from UniProt:P06959
aceF(del)::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
aceF(del)::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
aceF10			Auxotrophies			CGSC:5708
aceF300::Tn10			Auxotrophies			CGSC:70083
aceF733(del)::kan	deletion	deletion	Auxotrophies		PMID:16738554	CGSC:99888
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0111	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTATCGAAATCAAAGTACC Primer 2:CCCATCACCAGACGGCGAATGTC
4D12	Kohara Phage	Genobase	PMID:3038334
4E11	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 16% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 43% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10025	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10025	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000022	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944794	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0024	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000400	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AceF
Synonyms	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2^[1], B0115^[2]^[1] , ECK0114, JW0111, b0115
Product description	AceF-lipoate^[2]^[3], AceF-S-acetyldihydrolipoate^[2]^[3] Pyruvate dehydrogenase, dihydrolipoamide acetyltransferase E2; acetate requirement^[4]
EC number (for enzymes)	2.3.1.12^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004742	dihydrolipoyllysine-residue acetyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006256	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004742	dihydrolipoyllysine-residue acetyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.1.12	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004167	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006256	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0324	P	Seeded from EcoCyc (v14.0)	complete
	GO:0031405	lipoic acid binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0450	F	Seeded from EcoCyc (v14.0)	complete
	GO:0031405	lipoic acid binding	PMID:12651118^[7]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0045254	pyruvate dehydrogenase complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006256	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:16858726^[8]	IDA: Inferred from Direct Assay		C		complete
	GO:0045254	pyruvate dehydrogenase complex	PMID:3903169^[9]	IDA: Inferred from Direct Assay		C		complete
	GO:0045254	pyruvate dehydrogenase complex	PMID:327021^[10]	IGI: Inferred from Genetic Interaction	EcoliWiki:lpd EcoliWiki:acE	C		complete
	GO:0004742	dihydrolipoyllysine-residue acetyltransferase activity	PMID:12651118^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0031405	lipoic acid binding	PMID:12651118^[7]	IDA: Inferred from Direct Assay		F	Lysine at aa 425 is lipoylated	complete
Contributes to	GO:0004738	pyruvate dehydrogenase activity	PMID:3903169^[9]	IDA: Inferred from Direct Assay		F		complete
	GO:0006090	pyruvate metabolic process	PMID:349114^[11]	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0006086	acetyl-CoA biosynthetic process from pyruvate	PMID:349114^[11]	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dcm	PMID:15690043^[12]	Experiment(s):EBI-889144, EBI-894157
Protein	rpmF	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	sdhA	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	lpdA	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	rplV	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	acnB	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	aceE	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	rplY	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	rpmH	PMID:16606699^[13]	Experiment(s):EBI-1135600
Protein	mukB	PMID:16606699^[13]	Experiment(s):EBI-1135600
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Cytoplasm		PMID:9298646	EchoLocation:aceF
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAIEIKVPDI GADEVEITEI LVKVGDKVEA EQSLITVEGD KASMEVPSPQ AGIVKEIKVS VGDKTQTGAL IMIFDSADGA ADAAPAQAEE KKEAAPAAAP AAAAAKDVNV PDIGSDEVEV TEILVKVGDK VEAEQSLITV EGDKASMEVP APFAGTVKEI KVNVGDKVST GSLIMVFEVA GEAGAAAPAA KQEAAPAAAP APAAGVKEVN VPDIGGDEVE VTEVMVKVGD KVAAEQSLIT VEGDKASMEV PAPFAGVVKE LKVNVGDKVK TGSLIMIFEV EGAAPAAAPA KQEAAAPAPA AKAEAPAAAP AAKAEGKSEF AENDAYVHAT PLIRRLAREF GVNLAKVKGT GRKGRILRED VQAYVKEAIK RAEAAPAATG GGIPGMLPWP KVDFSKFGEI EEVELGRIQK ISGANLSRNW VMIPHVTHFD KTDITELEAF RKQQNEEAAK RKLDVKITPV VFIMKAVAAA LEQMPRFNSS LSEDGQRLTL KKYINIGVAV DTPNGLVVPV FKDVNKKGII ELSRELMTIS KKARDGKLTA GEMQGGCFTI SSIGGLGTTH FAPIVNAPEV AILGVSKSAM EPVWNGKEFV PRLMLPISLS FDHRVIDGAD GARFITIINN TLSDIRRLVM
Length	630
Mol. Wt	66.095 kDa
pI	5.0 (calculated)
Extinction coefficient	20,970 - 21,095 (calc based on 3 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P06959
Domain	397..630	PF00198 2-oxoacid dehydrogenases acyltransferase (catalytic domain)		PMID:19920124^[14]
Domain	3..74	PF00364 Biotin-requiring enzyme		PMID:19920124^[14]
Domain	106..177	PF00364 Biotin-requiring enzyme		PMID:19920124^[14]
Domain	207..278	PF00364 Biotin-requiring enzyme		PMID:19920124^[14]
Domain	325..363	PF02817 e3 binding domain		PMID:19920124^[14]
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=aceF taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128108	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944794	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000400	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P06959	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10025	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10025	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944794	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000022	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0024	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	8.02E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	850.657+/-6.457	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.2289+/-0.02836	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.906976744	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	60155	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5226	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	28493	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aceEF
pdhR-aceEF-lpdA
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:125675..125715 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0115 (EcoliWiki Page)	NCBI GEO profiles for aceF
microarray	GenExpDB:b0115 (EcoliWiki Page)	Summary of data for aceF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aceF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10025	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0024	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0115
EcoGene	EcoGene:EG10025	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000022	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000400	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000023896 (score: 1.000; bootstrap: 85%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000032195 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00026169 (score: 1.000; bootstrap: 80%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00014054 (score: 1.000; bootstrap: 82%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000003451 (score: 1.000; bootstrap: 55%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030131-2921 (score: 1.000; bootstrap: 64%) ZDB-CDNA-040425-2474 (score: 0.126) ZDB-CDNA-040425-2473 (score: 0.123) ZDB-GENE-040426-1539 (score: 0.123)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230195 (score: 1.000; bootstrap: 96%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0030612 (score: 1.000; bootstrap: 59%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA18998-PA (score: 1.000; bootstrap: 89%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000010123 (score: 1.000; bootstrap: 63%)	From Inparanoid:20070104
Mus musculus	MGI:2385311 (score: 1.000; bootstrap: 64%) MGI:1351627 (score: 0.119)	From Inparanoid:20070104
Oryza gramene	Q655Q2 (score: 1.000; bootstrap: 51%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000032890 (score: 1.000; bootstrap: 62%)	From Inparanoid:20070104
Shigella flexneri	ACEF	From SHIGELLACYC
E. coli O157	ACEF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02817 e3 binding domain
Pfam (EcoliWiki Page)	PF00364 Biotin-requiring enzyme
Pfam (EcoliWiki Page)	PF00198 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Pfam (EcoliWiki Page)	PF00364 Biotin-requiring enzyme
Pfam (EcoliWiki Page)	PF00364 Biotin-requiring enzyme
Superfamily (EcoliWiki Page)	SUPERFAMILY:47005
Superfamily (EcoliWiki Page)	SUPERFAMILY:51230
Superfamily (EcoliWiki Page)	SUPERFAMILY:51230
Superfamily (EcoliWiki Page)	SUPERFAMILY:51230
Panther (EcoliWiki Page)	PTHR23151:SF19
Superfamily (EcoliWiki Page)	SUPERFAMILY:52777
EcoCyc	EcoCyc:EG10025	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10025	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000022	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0024	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000400	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 Wei, W et al. (2003) Expression and purification of the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase subunits of the Escherichia coli pyruvate dehydrogenase multienzyme complex: a mass spectrometric assay for reductive acetylation of dihydrolipoamide acetyltransferase. Protein Expr. Purif. 28 140-50 PubMed
↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
↑ ^9.0 ^9.1 Guest, JR et al. (1985) Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. J. Mol. Biol. 185 743-54 PubMed
↑ Langley, D & Guest, JR (1977) Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. J. Gen. Microbiol. 99 263-76 PubMed
↑ ^11.0 ^11.1 Langley, D & Guest, JR (1978) Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants. J. Gen. Microbiol. 106 103-17 PubMed
↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
↑ ^13.0 ^13.1 ^13.2 ^13.3 ^13.4 ^13.5 ^13.6 ^13.7 ^13.8 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ ^14.0 ^14.1 ^14.2 ^14.3 ^14.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

aceF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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