abgT:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

<protect>

Standard Name

abgT

Gene Synonym(s)

ECK1332, b1336, JW5822, ydaH[1], ydaH

Product Desc.

AbgT Transporter[2][3]

p-Aminobenzoyl-glutamate transporter; membrane protein[4]

Product Synonyms(s)

predicted cryptic aminobenzoyl-glutamate transporter[1], B1336[2][1], YdaH[2][1], AbgT[2][1] , ECK1332, JW5822, ydaH, b1336

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ogt[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]


Artificial expression of abgT allows the uptake of p-aminobenzoyl-glutamate (Hussein, 1998; Carter, 2007). abgAB expression catalyzes the p-aminobenzoyl-glutamate assimilation to feed p-aminobenzoate auxotrophs (Carter, 2007). The abg operon is silent and an AbgR inducer has not been found, suggesting another natural substrate (Hussein, 1998; Carter, 2007). IT (Ion Transporter) superfamily (Prakash, 2003). ST(3) structural class based on hydropathy profile (Lolkema, 2003).[4]



Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

abgT

Mnemonic

Aminobenzoyl-glutamate

Synonyms

ECK1332, b1336, JW5822, ydaH[1], ydaH

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

30.14 minutes 

MG1655: 1399797..1398271
<gbrowseImage> name=NC_000913:1398271..1399797 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1398943..1397417
<gbrowseImage> name=NC_012967:1397417..1398943 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1290330..1291856
<gbrowseImage> name=NC_012759:1290330..1291856 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1403487..1401961
<gbrowseImage> name=NC_007779:1401961..1403487 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1489193..1487667
<gbrowseImage> name=NC_010473:1487667..1489193 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔabgT (Keio:JW5822)

deletion

deletion

PMID:16738554

Shigen

ydaH::Tn5KAN-I-SceI (FB20297)

Insertion at nt 305 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20297

contains pKD46

ΔydaH::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5822

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTATGTCATCCATACCGTC

Primer 2:CCAGACAAACGTGGGTAAATACC

6B4

Kohara Phage

Genobase

PMID:3038334

3G3

Kohara Phage

Genobase

PMID:3038334

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[5]

est. P1 cotransduction: 32% [6]
Synonyms:zci-233::Tn10

recT3061::Tn10

Linked marker

CAG12081 = CGSC7375[5]

est. P1 cotransduction: 66% [6]
Synonyms:zcj-3061::Tn10, zda-3061::Tn10

pMJH128

Plasmid Clone

plasmid carrying abgT restores the p-aminobenzoyl-glutamate untilization phenotype of the abg::kan strain [7]

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12853

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12853

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002401

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945912

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2697

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004488

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AbgT

Synonyms

predicted cryptic aminobenzoyl-glutamate transporter[1], B1336[2][1], YdaH[2][1], AbgT[2][1] , ECK1332, JW5822, ydaH, b1336

Product description

AbgT Transporter[2][3]

p-Aminobenzoyl-glutamate transporter; membrane protein[4]

EC number (for enzymes)


<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006865

amino acid transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0029

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MPMSMSSIPS SSQSGKLYGW VERIGNKVPH PFLLFIYLII VLMVTTAILS AFGVSAKNPT
DGTPVVVKNL LSVEGLHWFL PNVIKNFSGF APLGAILALV LGAGLAERVG LLPALMVKMA
SHVNARYASY MVLFIAFFSH ISSDAALVIM PPMGALIFLA VGRHPVAGLL AAIAGVGCGF
TANLLIVTTD VLLSGISTEA AAAFNPQMHV SVIDNWYFMA SSVVVLTIVG GLITDKIIEP
RLGQWQGNSD EKLQTLTESQ RFGLRIAGVV SLLFIAAIAL MVIPQNGILR DPINHTVMPS
PFIKGIVPLI ILFFFVVSLA YGIATRTIRR QADLPHLMIE PMKEMAGFIV MVFPLAQFVA
MFNWSNMGKF IAVGLTDILE SSGLSGIPAF VGLALLSSFL CMFIASGSAI WSILAPIFVP
MFMLLGFHPA FAQILFRIAD SSVLPLAPVS PFVPLFLGFL QRYKPDAKLG TYYSLVLPYP
LIFLVVWLLM LLAWYLVGLP IGPGIYPRLS
Length

510

Mol. Wt

55.116 kDa

pI

9.5 (calculated)

Extinction coefficient

61,880 - 62,130 (calc based on 12 Y, 8 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

14..507

PF03806 AbgT putative transporter family

PMID:19920124

<motif_map/>

tmhmm.php?gene=abgT&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=abgT taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111251

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945912

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004488

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P46133

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12853

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12853

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945912

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002401

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2697

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]




Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

3

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ogt

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<protect><gbrowseImage> name=NC_000913:1399777..1399817 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1336 (EcoliWiki Page)

NCBI GEO profiles for abgT

microarray

GenExpDB:b1336 (EcoliWiki Page)

Summary of data for abgT from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

<protect></protect>

Notes

Accessions Related to abgT Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12853

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2697

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1336

EcoGene

EcoGene:EG12853

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002401

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004488

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]


<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

</protect>


Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03806 AbgT putative transporter family

EcoCyc

EcoCyc:EG12853

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12853

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002401

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2697

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004488

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Hussein, MJ et al. (1998) Characterization of mutations that allow p-aminobenzoyl-glutamate utilization by Escherichia coli. J. Bacteriol. 180 6260-8 PubMed

Categories

[back to top]