degP:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

degP

Mnemonic

Degradative protease

Synonyms

ECK0160, b0161, JW0157, htrA, ptd[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

3.9 minutes 

MG1655: 180884..182308
<gbrowseImage> name=NC_000913:180884..182308 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 183726..185150
<gbrowseImage> name=NC_012967:183726..185150 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 180883..182307
<gbrowseImage> name=NC_012759:180883..182307 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 180884..182308
<gbrowseImage> name=AC_000091:180884..182308 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 154988..156412
<gbrowseImage> name=NC_010473:154988..156412 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

180962

Edman degradation

PMID:2180903[2]
PMID:9600841[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdegP (Keio:JW0157)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC8424[5]

ΔhtrA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[6]

degP41(Δ(PstI))::kan

CGSC:92707

ΔdegP775::kan

PMID:16738554[4]

CGSC:100570



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0157

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAAAAAACCACATTAGCACT

Primer 2:CCCTGCATTAACAGGTAGATGGT

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 28% [8]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 9% [8]
Synonyms:zae-502::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10463

EchoBASE (EcoliWiki Page)

EchoBASE:EB0458

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0161

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0197

EcoGene (EcoliWiki Page)

EcoGene:EG10463

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HTRA

CGSC (EcoliWiki Page)

CGSC:30554

ASAP (EcoliWikiPage)

ASAP:ABE-0000551



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Lipinska, B et al. (1990) The HtrA (DegP) protein, essential for Escherichia coli survival at high temperatures, is an endopeptidase. J. Bacteriol. 172 1791-7 PubMed
  3. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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