ompC:Gene

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ompC

Mnemonic

Outer membrane protein

Synonyms

ECK2207, b2215, JW2203, meoA, par[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

49.78 minutes, 49.78 minutes 

MG1655: 2310771..2309668
gbrowse_img.php?coord=2309668..2310771&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2262685..2261750
gbrowse_img.php?coord=2261750..2262685&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2195473..2196576
gbrowse_img.php?coord=2195473..2196576&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2316083..2314980
gbrowse_img.php?coord=2314980..2316083&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2401759..2400656
gbrowse_img.php?coord=2400656..2401759&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2309671

Edman degradation

PMID:6297988[2]
PMID:9298646[3]
PMID:9629924[4]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔompC (Keio:JW2203)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC9781[6]

ompC

PMID:12519969[7]

ASAP

At position 469 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20710

ompC

PMID:12519969[7]

ASAP

At position 469 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20711

ompC171

CGSC:6820

ompC161

CGSC:8752

ompC263

CGSC:9903

ompC264

CGSC:9913

ompC262

CGSC:9918

ompC172

CGSC:14635

ΔompC768::kan

PMID:16738554[5]

CGSC:102176



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2203

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAAGTTAAAGTACTGTCCCT

Primer 2:CCGAACTGGTAAACCAGACCCAG

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[6]

est. P1 cotransduction: 75% [9]
Synonyms:zeg-722, zei-722::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[6]

est. P1 cotransduction: 71% [9]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10670

EchoBASE (EcoliWiki Page)

EchoBASE:EB0664

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2215

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2758

EcoGene (EcoliWiki Page)

EcoGene:EG10670

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:OMPC

CGSC (EcoliWiki Page)

CGSC:436

ASAP (EcoliWikiPage)

ASAP:ABE-0007321



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Mizuno, T et al. (1983) DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro-OmpC protein of Escherichia coli. FEBS Lett. 151 159-64 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Molloy, MP et al. (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 837-44 PubMed
  5. ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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