PMID:6297988

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Citation

Mizuno, T, Chou, MY and Inouye, M (1983) DNA sequence of the promoter region of the ompC gene and the amino acid sequence of the signal peptide of pro-OmpC protein of Escherichia coli. FEBS Lett. 151:159-64

Abstract

The osmoregulated ompC gene of Escherichia coli was cloned and the DNA sequence of a fragment encompassing the promoter region and a portion of the coding region was determined. There were no obvious homologies in the DNA sequences of the promoter regions of the ompC and ompF genes, in contrast to those of the coding regions of the two genes, both of which code for the matrix porins (major outer membrane proteins) and form passive diffusion pores. The amino acid sequence of the signal peptide of pro-OmpC protein was also deduced from the DNA sequence.

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Keywords

Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacteriophage lambda/genetics; Bacteriophages/genetics; Cloning, Molecular; DNA Restriction Enzymes; DNA, Bacterial; DNA, Viral; Escherichia coli/genetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Nucleic Acid Hybridization; Operon; Peptides; Protein Sorting Signals; Repetitive Sequences, Nucleic Acid

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