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!align=left {{table_heading_style}} |Standard name
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''adhE''  
''adhE''  
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!align=left {{table_heading_style}} |Mnemonic
!align=left |Mnemonic
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!align=left {{table_heading_style}} |Synonyms
!align=left |Synonyms
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''ECK1235, b1241, JW1228, adhC, ana''<ref name='LIB:Riley_2006'/>
''ECK1235, b1241, JW1228, adhC, ana''<ref name='LIB:Riley_2006'/>


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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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{{GBrowseFastaLink|ecoli|NC_000913|1294669|1297344|FASTA Dump}}
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*[http://biocyc.org/ECOLI/sequence?object=EG10031 EcoCyc Sequence Dump]  
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[[REL606]]
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NC_012967: 1294239..1296914<br />{{GBrowseFigureByCoord|1294239|1296914|REL606|NC_012967}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|1329560|1342935|FASTA Dump}}  
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[[REL606]]
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NC_012967: 1294239..1296914<br />{{GBrowseFigureByCoord|1294239|1296914|REL606|NC_012967}}
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Synonyms:zch-506::Tn10, zci-506::Tn10  
Synonyms:zch-506::Tn10, zci-506::Tn10  


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Revision as of 23:54, 8 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

adhE

Mnemonic
Synonyms

ECK1235, b1241, JW1228, adhC, ana[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.9 minutes 

MG1655: 1297344..1294669
gbrowse_img.php?coord=1294669..1297344&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1294239..1296914
gbrowse_img.php?coord=1294239..1296914&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1299698..1297023
gbrowse_img.php?coord=1297023..1299698&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1337585..1334910
gbrowse_img.php?coord=1334910..1337585&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔadhE (Keio:JW1228)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9113[3]

adhE

PMID:12519969[4]

ASAP

At position 1923 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20272

ana-1

CGSC:5096

adhE80

PMID:6986356[5]

CGSC:7028

adhE1

PMID:6752127[6]

CGSC:7285

adhE16(ts)

temperature sensitive

CGSC:7289

adhE2

PMID:6752127[6]

CGSC:7457

ana-2

adhE

CGSC:14693

adhE36

PMID:3550385[7]

CGSC:92529

adhE38

PMID:3550385[7]

CGSC:92530

ΔadhE748::kan

PMID:16738554[2]

CGSC:99914


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1228

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGCTGTTACTAATGTCGCTGA

Primer 2:CCAGCGGATTTTTTCGCTTTTTT

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 4% [9]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[3]

est. P1 cotransduction: 73% [9]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10031

EchoBASE (EcoliWiki Page)

EchoBASE:EB0030

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1241

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1705

EcoGene (EcoliWiki Page)

EcoGene:EG10031

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ADHE

CGSC (EcoliWiki Page)

CGSC:1041

ASAP (EcoliWikiPage)

ASAP:ABE-0004164


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Clark, D & Cronan, JE Jr (1980) Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J. Bacteriol. 141 177-83 PubMed
  6. ↑ 6.0 6.1 Lorowitz, W & Clark, D (1982) Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase. J. Bacteriol. 152 935-8 PubMed
  7. ↑ 7.0 7.1 Cunningham, PR & Clark, DP (1986) The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol. Gen. Genet. 205 487-93 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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