acpT:Gene: Difference between revisions

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Primer 2:CCGTTAACTGAATCGATCCATTG  
Primer 2:CCGTTAACTGAATCGATCCATTG  
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zhg-50::Tn10
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Linked marker
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CAG18450 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37863 CGSC7450]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 49% <ref name='LIB:Nichols_linked_Tn'/>
Synonyms:zhf-50::Tn10, zhf-5::Tn10
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zic-4901::Tn10
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Linked marker
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CAG18492 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38944 CGSC7457]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>
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Revision as of 23:23, 28 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

acpT

Mnemonic
Synonyms

ECK3459, b3475, JW3440, yhhU[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

77.83 minutes 

MG1655: 3610992..3611579
gbrowse_img.php?coord=3609992..3612579&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 4027446..4026859
gbrowse_img.php?coord=4025859..4028446&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability

ΔacpT (Keio:JW3440)

deletion

deletion

PMID:16738554[2]

Shigen

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW3440

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCTATCGGATAGTTCTGGGGAA

Primer 2:CCGTTAACTGAATCGATCCATTG

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: 49% [5]

Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: % [5]

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12221

EchoBASE (EcoliWiki Page)

EchoBASE:EB2135

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3475

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4268

EcoGene (EcoliWiki Page)

EcoGene:EG12221

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z4867

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).