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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10235 ECOCYC:EG10235]
EcoCyc:EG10235
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10235 EcoGene:EG10235]
EcoGene:EG10235
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[RegulonDB]]
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RegulonDB:ECK120000229
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB0231
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0012103
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:32, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaA

Mnemonic

DNA

Synonyms

ECK3694, b3702, JW3679[1], JW3679

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.63 minutes 

MG1655: 3881752..3880349
<gbrowseImage> name=NC_000913:3880349..3881752 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3844090..3842687
<gbrowseImage> name=NC_012967:3842687..3844090 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3768682..3770085
<gbrowseImage> name=NC_012759:3768682..3770085 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3756686..3758089
<gbrowseImage> name=AC_000091:3756686..3758089 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

P18S

Nonsynonomous mutation

PMID:18245285[2]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

dnaA177(ts)

temperature sensitive

CGSC:5231

dnaA46

A184V and H252Y

Growth Phenotype

Temperature-sensitive for initiation of DNA replication.

PMID:1495477[3]

dnaA508(ts)

temperature sensitive

PMID:6094968[4]

CGSC:5272

dnaA5(ts)

temperature sensitive

PMID:321430[5] PMID:6094968[4]

CGSC:7325

dnaA46(ts)

783 C->T (where +1 is the txn'l start site)

Ala->Val

Growth Phenotype

temperature sensitive

PMID:6094968[4]

CGSC:73497

Sequence of the mutant allele is from Ohmori et al.[6]

dnaAcos

dnaA1(Cs) 699 A->T dnaA46(Ts) 783 C->T dnaA2(Cs) 986 C->T dnaA3(Cs) 1043 T->C (Where +1 is the txn'l start site)

Gln->Leu Ala->Val His->Tyr Tyr->His

Growth Phenotype

over initiation of DNA replication at 30°C

PMID:9379896[7]

dnaAcos was isolated as a temperature-resistant revertant of dnaA46(Ts). [8]

dnaA W6A

W6A

PMID:15878847[9]

W6 is needed for oligomerization

dnaA311(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354[10]

suppressed in supF, supE, supD, and supP strains

dnaA366(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354[10]

  • ts in supF
  • suppressed in supE and supD strains

dnaA91(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354[10]

suppressed in supF, supD, and supP strains

dnaA204(Ts)

Resistant to

blocks resistance to camphor in all mbr mutants

PMID:2176633[11] PMID:6094968[4]

dnaA167

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA211

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA606

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA601

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA604

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA603

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA205

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA602

Growth Phenotype

temperature sensitive

PMID:6094968[4]

dnaA203

Growth Phenotype

temperature sensitive

PMID:6094968[4]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3679

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCTCACTTTCGCTTTGGCAGCA

Primer 2:CCaGATGACAATGTTCTGATTAA

3F11

Kohara Phage

Genobase

PMID:3038334[13]

3C10

Kohara Phage

Genobase

PMID:3038334[13]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[14]

est. P1 cotransduction: 81% [15]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[14]

est. P1 cotransduction: 10% [15]
Synonyms:zie-296::Tn10

tna::Tn10

Linked marker

WP72[10]

  • tryptophanase minus
  • tetracycline resistant
  • est. P1 cotransduction: 85%
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10235

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10235

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000229

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0231

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012103

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  3. ↑ Hansen, FG et al. (1992) Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli. Mol. Gen. Genet. 234 14-21 PubMed
  4. ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 Hansen, EB et al. (1984) Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol. Gen. Genet. 196 387-96 PubMed
  5. ↑ Zyskind, JW & Smith, DW (1977) NOVEL Escherichia coli dnaB mutant: direct involvement of the dnaB252 gene product in the synthesis of an origin-ribonucleic acid species during initiaion of a round of deoxyribonucleic acid replication. J. Bacteriol. 129 1476-86 PubMed
  6. ↑ Ohmori, H et al. (1984) Structural analysis of the dnaA and dnaN genes of Escherichia coli. Gene 28 159-70 PubMed
  7. ↑ Katayama, T et al. (1997) Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product. Mol. Microbiol. 25 661-70 PubMed
  8. ↑ Kellenberger-Gujer, G et al. (1978) A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature. Mol. Gen. Genet. 162 9-16 PubMed
  9. ↑ Felczak, MM et al. (2005) An essential tryptophan of Escherichia coli DnaA protein functions in oligomerization at the E. coli replication origin. J. Biol. Chem. 280 24627-33 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 Schaus, N et al. (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145 904-13 PubMed
  11. ↑ Trun, NJ & Gottesman, S (1990) On the bacterial cell cycle: Escherichia coli mutants with altered ploidy. Genes Dev. 4 2036-47 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 CGSC: The Coli Genetics Stock Center
  15. ↑ 15.0 15.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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