dnaK:Gene: Difference between revisions

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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=101 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=101 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:6H3|6H3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=102 Genobase]
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PMID:3038334  
PMID:3038334  
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est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:car-96::Tn10  
Synonyms:car-96::Tn10  
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[[:Category:Kohara_Phage:6H3|6H3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=102 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10241 EcoCyc:EG10241]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0237 EchoBASE:EB0237]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0014 EcoliGenExpDB:b0014]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C0019 ECOL199310CYC:C0019]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10241 EcoGene:EG10241]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=DNAK ECOO157CYC:DNAK]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/844 CGSC:844]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0000052&formSubmitReturn=1 ASAP:ABE-0000052]
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Revision as of 19:55, 21 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaK

Mnemonic

DNA

Synonyms

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.26 minutes 

MG1655: 12163..14079
<gbrowseImage> name=NC_000913:12163..14079 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 12161..14077
<gbrowseImage> name=NC_012967:12161..14077 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 12163..14079
<gbrowseImage> name=NC_012759:12163..14079 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 12163..14079
<gbrowseImage> name=AC_000091:12163..14079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 12163..14079
<gbrowseImage> name=NC_010473:12163..14079 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

12166

Edman degradation

PMID:1396676[3]
PMID:7783627[4]
PMID:9298646[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdnaK (Keio:JW0013)

deletion

deletion

PMID:16738554[6]

Shigen
CGSC8342[7]

dnaKG32D

G32D

In SEG-1 and dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKV436I

V436I

In mutant SEG-2; confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG455D

G455D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG468D

G468D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaK756(ts)

temperature sensitive

CGSC:6707

ΔdnaK734::kan

deletion

deletion

PMID:16738554[6]

CGSC:100628


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0013

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCGGTAAAATAATTGGTATCGA

Primer 2:CCTTTTTTGTCTTTGACTTCTTC

9E4

Kohara Phage

Genobase

PMID:3038334[9]

6H3

Kohara Phage

Genobase

PMID:3038334[9]

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[7]

est. P1 cotransduction: 66% [10]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[7]

est. P1 cotransduction: 48% [10]
Synonyms:car-96::Tn10

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Notes

Sugimoto et al [11] constructed a strain carrying a dnaK deletion and the lambda DE3 prophage, which expresses T7 RNA polymerase under lac control.

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Schmid, D et al. (1992) Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat-shock protein DnaK. Eur. J. Biochem. 208 699-704 PubMed
  4. ↑ Freestone, P et al. (1995) Identification of phosphoproteins in Escherichia coli. Mol. Microbiol. 15 573-80 PubMed
  5. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. ↑ 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  11. ↑ Sugimoto, S et al. (2008) Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins. Protein Expr. Purif. 60 31-6 PubMed

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