iscS:Gene: Difference between revisions

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Revision as of 20:06, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

iscS

Mnemonic

Iron-sulfur cluster

Synonyms

ECK2527, b2530, JW2514, csdB, yfhO, nifS, yzzO, nuvC?[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.3 minutes 

MG1655: 2659553..2658339
<gbrowseImage> name=NC_000913:2658339..2659553 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2582230..2581016
<gbrowseImage> name=NC_012967:2581016..2582230 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2544144..2545358
<gbrowseImage> name=NC_012759:2544144..2545358 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2660187..2658973
<gbrowseImage> name=AC_000091:2658973..2660187 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2751318..2750104
<gbrowseImage> name=NC_010473:2750104..2751318 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2658339

Edman degradation

PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔiscS (Keio:JW2514)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10002[5]

iscSC328A

C328A

Loss of cysteine desulfurization

seeded from UniProt:P0A6B7

ΔiscS776::kan

PMID:16738554[4]

CGSC:101588


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2514

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAATTACCGATTTATCTCGA

Primer 2:CCATGATGAGCCCATTCGATGCT

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 3% [7]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 85% [7]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7325

EchoBASE (EcoliWiki Page)

EchoBASE:EB2542

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2530

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3056

EcoGene (EcoliWiki Page)

EcoGene:EG12677

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YFHO

ASAP (EcoliWikiPage)

ASAP:ABE-0008324


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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