acpT:Gene: Difference between revisions

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Auxotrophies
Auxotrophies
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acpT can functionally replace acpS only if over-expressed. Fig. 8, plate 2. Fig. 9, lane 3.  
acpT can functionally replace [[acpS:Quickview|acpS]] only if over-expressed. Fig. 8, plate 2. Fig. 9, lane 3.  
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PMID:10625633
PMID:10625633

Revision as of 21:40, 8 July 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

acpT

Mnemonic

Acyl carrier protein

Synonyms

ECK3459, b3475, JW3440, yhhU[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

77.83 minutes 

MG1655: 3610992..3611579
<gbrowseImage> name=NC_000913:3610992..3611579 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3543657..3544244
<gbrowseImage> name=NC_012967:3543657..3544244 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3499486..3500073
<gbrowseImage> name=NC_012759:3499486..3500073 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4027446..4026859
<gbrowseImage> name=AC_000091:4026859..4027446 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3708737..3709324
<gbrowseImage> name=NC_010473:3708737..3709324 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔacpT (Keio:JW3440)

deletion

deletion

PMID:16738554[2]

Shigen

acpT over-expression

Auxotrophies

acpT can functionally replace acpS only if over-expressed. Fig. 8, plate 2. Fig. 9, lane 3.

PMID:10625633[3]



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3440

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTATCGGATAGTTCTGGGGAA

Primer 2:CCGTTAACTGAATCGATCCATTG

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[5]

est. P1 cotransduction: 49% [6]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[5]

est. P1 cotransduction: % [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12221

EchoBASE (EcoliWiki Page)

EchoBASE:EB2135

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3475

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4268

EcoGene (EcoliWiki Page)

EcoGene:EG12221

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z4867

ASAP (EcoliWikiPage)

ASAP:ABE-0011351



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Flugel, RS et al. (2000) Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli. J. Biol. Chem. 275 959-68 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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