dinB:Gene: Difference between revisions

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Fixing Blattner deletion strains in alleles table.
Adding section & Genetic interactions table to Gene pages.
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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:22, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dinB

Mnemonic

damage inducible Damage inducible

Synonyms

ECK0232, b0231, JW0221, dinP[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.41 minutes 

MG1655: 250898..251953
<gbrowseImage> name=NC_000913:250898..251953 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 255461..256516
<gbrowseImage> name=NC_012967:255461..256516 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 250897..251952
<gbrowseImage> name=NC_012759:250897..251952 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 250898..251953
<gbrowseImage> name=AC_000091:250898..251953 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 225002..226057
<gbrowseImage> name=NC_010473:225002..226057 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdinB (Keio:JW0221)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8456[3]

dinP::Tn5KAN-I-SceI (FB20062)

Insertion at nt 89 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20062

does not contain pKD46

dinP::Tn5KAN-I-SceI (FB20063)

Insertion at nt 89 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20063

contains pKD46

dinBA310S

A310S

(in strain: ECOR 57B2, ECOR 59B2, ECOR 60B2 and ECOR 62B2)

Strain variation; seeded from UniProt:Q47155

dinBD321N

D321N

(in strain: ECOR 35D)

Strain variation; seeded from UniProt:Q47155

dinBD8A,H

D8A,H

Loss of function

seeded from UniProt:Q47155

dinBR49A,F

R49A,F

Loss of function

seeded from UniProt:Q47155

dinBD103A,N

D103A,N

Loss of function

seeded from UniProt:Q47155

dinBE104A

E104A

Loss of function

seeded from UniProt:Q47155

dinBM210I

M210I

(in strain: ECOR 37UG, ECOR 45B1, ECOR 51B2, ECOR 52B2, ECOR 58B1 and ECOR 70B1)

Strain variation; seeded from UniProt:Q47155

dinBM210T

M210T

(in strain: ECOR 35D, ECOR 46D, ECOR 49D, ECOR 50D, ECOR 57B2, ECOR 59B2, ECOR 60B2 and ECOR 62B2)

Strain variation; seeded from UniProt:Q47155

dinBR225C

R225C

(in strain: ECOR 59B2 and ECOR 60B2)

Strain variation; seeded from UniProt:Q47155

dinBERR36ARG

ERR36ARG

(in strain: ECOR 45B1)

Strain variation; seeded from UniProt:Q47155

dinBQ124K

Q124K

(in strain: ECOR 35D)

Strain variation; seeded from UniProt:Q47155

dinBN132S

N132S

(in strain: ECOR 34B1 and ECOR 37UG)

Strain variation; seeded from UniProt:Q47155

dinBQ135H

Q135H

(in strain: ECOR 70B1)

Strain variation; seeded from UniProt:Q47155

dinBP170S

P170S

(in strain: ECOR 37UG)

Strain variation; seeded from UniProt:Q47155

dinBA171T

A171T

(in strain: ECOR 45B1, ECOR 46D, ECOR 49D and ECOR 50D)

Strain variation; seeded from UniProt:Q47155

dinBL176F

L176F

(in strain: ECOR 37UG)

Strain variation; seeded from UniProt:Q47155

dinBG201S

G201S

(in strain: ECOR 59B2)

Strain variation; seeded from UniProt:Q47155

dinB10

Other

xx

PMID:| SMR

we like this mutation a lot

F172A

PMID:18158902[5]

defective in UMuD interaction

ΔdinB749::kan

PMID:16738554[2]

CGSC:100600


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0221

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCCGTAAAATCATTCATGTGGA

Primer 2:CCTAATCCCAGCACCAGTTGTCT

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[3]

est. P1 cotransduction: 50% [7]
Synonyms:zae-502::Tn10

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[3]

est. P1 cotransduction: 74% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6115

EchoBASE (EcoliWiki Page)

EchoBASE:EB2935

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0231

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0379

EcoGene (EcoliWiki Page)

EcoGene:EG13141

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DINP

ASAP (EcoliWikiPage)

ASAP:ABE-0000789


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Godoy, VG et al. (2007) UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB. Mol. Cell 28 1058-70 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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