dnaX:Gene: Difference between revisions

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Revision as of 20:36, 26 July 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaX

Mnemonic

DNA

Synonyms

ECK0464, b0470, JW0459, dnaZ[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

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MG1655: 491316..493247
<gbrowseImage> name=NC_000913:491316..493247 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 464159..466090
<gbrowseImage> name=NC_012967:464159..466090 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 394075..396006
<gbrowseImage> name=NC_012759:394075..396006 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 491316..493247
<gbrowseImage> name=AC_000091:491316..493247 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 430647..432578
<gbrowseImage> name=NC_010473:430647..432578 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

491319

Edman degradation

PMID:3283125[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dnaX36

E601K

Growth Phenotype

ts growth. Fast stop DNA replication arrest at 44°

PMID:16430690[3] PMID:8376347[4]

Affects Tau but not Gamma

dnaX36

Other

Weak mutator in mismatch repair deficient strain

PMID:16430690[3]

dnaX0

CGSC:10600

dnaX2016

G118D

Growth Phenotype

Ts for replication and growth

PMID:8376347[4]

CGSC:10601

dnaX36(ts)

temperature sensitive

CGSC:10704

dnaXE145A

E145A

failure to suppress parE10 when overexpressed

PMID:12535532[5]

dnaX(Ts)

G118D

Growth Phenotype

temperature sensitive for replication and growth

PMID:12775696[6]

phenotype suppressed by mutaions in DnaA

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Notes

The dnaX locus was identified by the J, Walker lab[7] as the site of one of several ts mutations in strain SG133, which was originally thought to contain a single mutation in dnaZ.[8]

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0459

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCAGTTATCAGGTCTTAGCCCG

Primer 2:CCAATGGGGCGGATACTTTCTTC

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[10]

est. P1 cotransduction: 28% [11]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[10]

est. P1 cotransduction: 72% [11]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10245

EchoBASE (EcoliWiki Page)

EchoBASE:EB0241

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0470

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0589

EcoGene (EcoliWiki Page)

EcoGene:EG10245

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DNAX

CGSC (EcoliWiki Page)

CGSC:838

ASAP (EcoliWikiPage)

ASAP:ABE-0001633


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Maki, S & Kornberg, A (1988) DNA polymerase III holoenzyme of Escherichia coli. I. Purification and distinctive functions of subunits tau and gamma, the dnaZX gene products. J. Biol. Chem. 263 6547-54 PubMed
  3. ↑ 3.0 3.1 Pham, PT et al. (2006) Mutator mutants of Escherichia coli carrying a defect in the DNA polymerase III tau subunit. Mol. Microbiol. 59 1149-61 PubMed
  4. ↑ 4.0 4.1 Blinkova, A et al. (1993) The Escherichia coli DNA polymerase III holoenzyme contains both products of the dnaX gene, tau and gamma, but only tau is essential. J. Bacteriol. 175 6018-27 PubMed
  5. ↑ Espeli, O et al. (2003) Temporal regulation of topoisomerase IV activity in E. coli. Mol. Cell 11 189-201 PubMed
  6. ↑ Blinkova, A et al. (2003) Suppression of temperature-sensitive chromosome replication of an Escherichia coli dnaX(Ts) mutant by reduction of initiation efficiency. J. Bacteriol. 185 3583-95 PubMed
  7. ↑ Henson, JM et al. (1979) Isolation and characterization of dnaX and dnaY temperature-sensitive mutants of Escherichia coli. Genetics 92 1041-59 PubMed
  8. ↑ Sevastopoulos, CG et al. (1977) Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication. Proc. Natl. Acad. Sci. U.S.A. 74 3485-9 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 CGSC: The Coli Genetics Stock Center
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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