aspA:Gene: Difference between revisions

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Revision as of 20:20, 26 July 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aspA

Mnemonic

Aspartate

Synonyms

ECK4133, b4139, JW4099[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.08 minutes, 94.08 minutes 

MG1655: 4366350..4364914
<gbrowseImage> name=NC_000913:4364914..4366350 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4347048..4345612
<gbrowseImage> name=NC_012967:4345612..4347048 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4303649..4305085
<gbrowseImage> name=NC_012759:4303649..4305085 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4373007..4371571
<gbrowseImage> name=AC_000091:4371571..4373007 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4466712..4465276
<gbrowseImage> name=NC_010473:4465276..4466712 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4364917

Edman degradation

PMID:2987841[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

aspAK327R

K327R

Reduces activity by 99.7%. Increases KM for aspartic acid 5-fold

seeded from UniProt:P0AC38

aspAR29A

R29A

No effect on Kcat. Increases KM for aspartic acid 40-fold

seeded from UniProt:P0AC38

aspAK55R

K55R

Loss of activity

seeded from UniProt:P0AC38

aspAH124L

H124L

Reduces activity by 30%

seeded from UniProt:P0AC38

aspAS143G

S143G

Reduces Kcat by 90%. Increases KM for aspartic acid 4-fold

seeded from UniProt:P0AC38

aspAS143T

S143T

Reduces Kcat by 98.5%. Increases KM for aspartic acid 2-fold

seeded from UniProt:P0AC38

aspAD10A

D10A

Loss of activity

seeded from UniProt:P0AC38

aspAD10N

D10N

Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold

seeded from UniProt:P0AC38

aspAR15A

R15A

No effect on Kcat. Increases KM for aspartic acid 2.5-fold

seeded from UniProt:P0AC38

aspAH26N

H26N

No effect on Kcat. Increases KM for aspartic acid 3-fold

seeded from UniProt:P0AC38

ΔaspA (Keio:JW4099)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8324[4]

aspA22

CGSC:5112

aspA23

CGSC:5113

ΔaspA765::kan

PMID:16738554[3]

CGSC:100110


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4099

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCAAACAACATTCGTATCGA

Primer 2:CCtTGTTCGCTTTCATCAGTATA

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[4]

est. P1 cotransduction: 76% [6]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[4]

est. P1 cotransduction: 4% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10095

EchoBASE (EcoliWiki Page)

EchoBASE:EB0093

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4139

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5222

EcoGene (EcoliWiki Page)

EcoGene:EG10095

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ASPA

CGSC (EcoliWiki Page)

CGSC:991

ASAP (EcoliWikiPage)

ASAP:ABE-0013551


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Takagi, JS et al. (1985) Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W. Nucleic Acids Res. 13 2063-74 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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