PMID:2987841
| Citation |
Takagi, JS, Ida, N, Tokushige, M, Sakamoto, H and Shimura, Y (1985) Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W. Nucleic Acids Res. 13:2063-74 |
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| Abstract |
The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-terminal regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence. |
| Links | |
| Keywords |
Amino Acid Sequence; Ammonia-Lyases/genetics; Aspartate Ammonia-Lyase/genetics; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes/diagnostic use; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Genes, Regulator; Genetic Linkage; Promoter Regions, Genetic |
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