lexA:Gene: Difference between revisions

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Table edited by Azweifel via TableEdit
Table edited by Azweifel via TableEdit
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''lexA269'' (Silent ''SalI'' site )
''lexA251'' (Silent SalI site )
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GTC->TTC
TCG->TTG
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V115F
S119L
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Formic acid was used as to mutagenize the strain.  
This allele was produced spontaneously and using hydroxylamine.  
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''lexA263'' (Silent SalI site )
''lexA269'' (Silent ''SalI'' site )
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GGG->GAG
GTC->TTC
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G117E
V115F
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Both formic acid and hydroxylamine were used as to mutagenize the strain.  
Formic acid was used as to mutagenize the strain.  
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|-  
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''lexA251'' (Silent SalI site )
''lexA280'' (Silent ''SalI'' site )
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TCG->TTG
ATG->TTG
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S119L
M120L
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This allele was produced spontaneously and using hydroxylamine.  
Formic acid was used as to mutagenize the strain.  
|-  
|-  
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''lexA280'' (Silent ''SalI'' site )
''lexA263'' (Silent SalI site )
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ATG->TTG
GGG->GAG
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|
M120L
G117E
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Formic acid was used as to mutagenize the strain.  
Both formic acid and hydroxylamine were used as to mutagenize the strain.  
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|-  
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Formic acid was used as to mutagenize the strain.  
Formic acid was used as to mutagenize the strain.  
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''lexA271-281''
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K156N/Q184H
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Ind- phenotype
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PMID:2834329
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Formic acid was used as to mutagenize the strain.
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''lexA271'' (Silent ''SalI'' and ''BglI'' site)
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AAG->AAT
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K156N
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Ind- phenotype
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PMID:2834329
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''lexA281'' (Silent ''SalI'' and ''BstI'' site)
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CAG->CAT
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Q184H
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Ind- phenotype
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PMID:2834329
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''lexA273-282''
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K156T/E184V
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Ind- phenotype
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PMID:2834329
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Formic acid was used as to mutagenize the strain.
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''lexA273'' (Silent ''SalI and BglI'' sites)
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AAG->ACG
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K156T
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Ind- phenotype
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PMID:2834329
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''lexA282'' (Silent ''SalI and BglI'' sites)
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GAA->GTA
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E170V
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Ind- phenotype
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PMID:2834329
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Revision as of 20:50, 8 July 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lexA

Mnemonic

Lambda excision

Synonyms

ECK4035, b4043, JW4003, exrA, tsl, umuA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.71 minutes 

MG1655: 4255138..4255746
gbrowse_img.php?coord=4255138..4255746&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4236047..4236655
gbrowse_img.php?coord=4236047..4236655&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 4193983..4194591
gbrowse_img.php?coord=4193983..4194591&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 4260705..4261313
gbrowse_img.php?coord=4260705..4261313&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4354834..4355442
gbrowse_img.php?coord=4354834..4355442&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

A description of the lexA gene may be found in Markham et al. (1981)[2].

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lexAG85D

G85D

(in lexA3, resistant to cleavage)

Strain variation; seeded from UniProt:P0A7C2

lexA107

CGSC:6267

lexA1(Ind-)

non-inducible SOS response

CGSC:9237

lexA41(ts)

temperature sensitive

CGSC:9601

lexA3(Ind-)

non-inducible SOS response

CGSC:9602

lexA51

CGSC:10280

lexA101

CGSC:11964

lexA102

CGSC:11979

lexA109

CGSC:11985

lexA103

CGSC:14425

lexA104

CGSC:14427

lexA105

CGSC:14429

lexA106

CGSC:14431

lexA108

CGSC:14439

lexA71::Tn5

CGSC:30813

lexA2

C461T

T154I

Sensitivity to

hypocleavable repressor leads to a sensitivity to DNA damage

PMID:3127383[3]

spr

PMID:2834329[4] PMID:7038393[5]

uninducible, not cleaved during SOS response

lexA264 (Silent SalI site )

GGT->GTT

G80V

Ind- phenotype

PMID:2834329[4]

Formic acid was used as the mutagen

lexA261 (Silent SalI site )

GGT->GAT

G80D

Ind- phenotype

PMID:2834329[4]

Hydroxylamine was used as the mutagen

lexA266 (Silent SalI site )

GTG->GAG

V82E

Ind- phenotype

PMID:2834329[4]

Formic acid was used as the mutagen

lexA265 (Silent SalI site )

GTG->ATG

V82M

Ind- phenotype

PMID:2834329[4]

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA262 (Silent SalI site )

GCT->GTT

A83V

Ind- phenotype

PMID:2834329[4]

Hydroxylamine were used as to mutagenize the strain.

lexA267 (Silent SalI site )

GCT->ACT

A83T

Ind- phenotype

PMID:2834329[4]

Both hydroxylamine and hydrazine were used as to mutagenize the strain.

lexA252 (Silent SalI site )

GCT->--

A83Δ

Ind- phenotype

PMID:2834329[4]

Spontaneous mutation

lexA260 (Silent SalI site )

GCC->ACC

A84T

Ind- phenotype

PMID:2834329[4]

Hydroxylamine was used as to mutagenize the strain.

lexA268 (Silent SalI site )

GCC->GAC

A84D

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA3 (Silent SalI site )

GGT->GAT

G85D

Ind- phenotype

PMID:2834329[4]

Hydrazine was used as to mutagenize the strain.

lexA252 (Silent SalI site )

GAA->AAA

A95K

Ind- phenotype

PMID:2834329[4]

Spontaneous mutation

lexA274 (Silent SalI site )

GGC->CCC

R114P

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA251 (Silent SalI site )

TCG->TTG

S119L

Ind- phenotype

PMID:2834329[4]

This allele was produced spontaneously and using hydroxylamine.

lexA269 (Silent SalI site )

GTC->TTC

V115F

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA280 (Silent SalI site )

ATG->TTG

M120L

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA263 (Silent SalI site )

GGG->GAG

G117E

Ind- phenotype

PMID:2834329[4]

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA259 (Silent SalI site )

ACC->ATC

T154I

Ind- phenotype

PMID:2834329[4]

Both hydrazine and hydroxylamine were used as to mutagenize the strain.

lexA270 (Silent SalI site )

AAG->AGG

K156R

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA272 (Silent SalI site )

AAG->ATG

K156M

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA271-281

K156N/Q184H

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA271 (Silent SalI and BglI site)

AAG->AAT

K156N

Ind- phenotype

PMID:2834329[4]

lexA281 (Silent SalI and BstI site)

CAG->CAT

Q184H

Ind- phenotype

PMID:2834329[4]

lexA273-282

K156T/E184V

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA273 (Silent SalI and BglI sites)

AAG->ACG

K156T

Ind- phenotype

PMID:2834329[4]

lexA282 (Silent SalI and BglI sites)

GAA->GTA

E170V

Ind- phenotype

PMID:2834329[4]


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4003

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAGCGTTAACGGCCAGGCA

Primer 2:CCaAGCCAGTCGCCGTTGCGAAT

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[7]

est. P1 cotransduction: 60% [8]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[7]

est. P1 cotransduction: 9% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10533

EchoBASE (EcoliWiki Page)

EchoBASE:EB0528

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4043

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5014

EcoGene (EcoliWiki Page)

EcoGene:EG10533

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:LEXA

CGSC (EcoliWiki Page)

CGSC:558

ASAP (EcoliWikiPage)

ASAP:ABE-0013241


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Markham, BE et al. (1981) Nucleotide sequence of the lexA gene of Escherichia coli K-12. Nucleic Acids Res. 9 4149-61 PubMed
  3. ↑ Peterson, KR et al. (1988) The Escherichia coli K-12 lexA2 gene encodes a hypocleavable repressor. J. Bacteriol. 170 1975-7 PubMed
  4. ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 4.24 4.25 Lin, LL & Little, JW (1988) Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170 2163-73 PubMed
  5. ↑ Froehlich, B (1981) Weigle reactivation of the single-stranded DNA phage f1. Mol. Gen. Genet. 184 416-20 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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