atpA:Gene: Difference between revisions

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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
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[[MG1655]]
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3916339
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Edman degradation
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PMID:9298646<br />
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Revision as of 19:22, 11 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

atpA

Mnemonic

ATP

Synonyms

ECK3727, b3734, JW3712, papA, uncA, unc[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.41 minutes 

MG1655: 3917880..3916339
gbrowse_img.php?coord=3916339..3917880&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3880223..3878682
gbrowse_img.php?coord=3878682..3880223&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 3804672..3806213
gbrowse_img.php?coord=3804672..3806213&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 3716824..3718365
gbrowse_img.php?coord=3716824..3718365&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4015464..4013923
gbrowse_img.php?coord=4013923..4015464&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3916339

Edman degradation

PMID:9298646[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

atpAK175E

K175E

Reduced activity and reduced ATP- binding

seeded from UniProt:P0ABB0

atpAK175I

K175I

Reduced activity and loss of ATP- binding

seeded from UniProt:P0ABB0

ΔatpA (Keio:JW3712)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10713[4]

atpA401

CGSC:9579

ΔatpA763::kan

PMID:16738554[3]

CGSC:100134



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3712

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCAACTGAATTCCACCGAAAT

Primer 2:CCCCAGGATTGGGTTGCTTTGAA

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[4]

est. P1 cotransduction: 16% [6]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[4]

est. P1 cotransduction: 62% [6]
Synonyms:zie-296::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10098

EchoBASE (EcoliWiki Page)

EchoBASE:EB0096

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3734

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4660

EcoGene (EcoliWiki Page)

EcoGene:EG10098

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ATPA

CGSC (EcoliWiki Page)

CGSC:33

ASAP (EcoliWikiPage)

ASAP:ABE-0012213



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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